ABSTRACT
Appendix-associated hernias are extremely rare. They have been described sporadically in the literature, mostly as inguinal hernias. Appendix-associated incisional hernias are even more unusual. High clinical awareness is needed as complications can arise if misdiagnosis or delay occurs. We present an 80-year-old man with acute appendicitis in an incisional hernia. After successful surgery, the patient made a full recovery.
Subject(s)
Appendectomy , Appendicitis/diagnosis , Herniorrhaphy/adverse effects , Incisional Hernia/diagnosis , Rare Diseases/diagnosis , Abdominal Pain/etiology , Abdominal Wall/surgery , Aged, 80 and over , Appendicitis/etiology , Appendicitis/surgery , Appendix/diagnostic imaging , Appendix/surgery , Hernia, Inguinal/surgery , Humans , Incisional Hernia/etiology , Incisional Hernia/surgery , Male , Nausea/etiology , Rare Diseases/etiology , Rare Diseases/surgery , Tomography, X-Ray Computed , Treatment Outcome , Vomiting/etiologyABSTRACT
Currently, nanomaterials had been used for several applications; one of them is as bio-markers. These nanomaterials contain fluorescent compounds as effective indicators for imaging and other applications in Biotechnology. In previous studies, we proposed a functionalized nanomaterial-based biomarker from silica and Eysenhardtia Polystachia, a medicinal tree known in Mexico as "palo azul" (Kidneywood). Our previous results showed the feasibility of the nanomaterial obtained as bio-marker. In this article, our purpose is to evaluate the effects of extraction solvents on fluorescence of that biomarker. The photoluminescence (PL) effect was evaluated at different pH (4, 7.4 and 8); four extraction solvents, ethanol, methanol, methanol-ethanol and methanol-ethanol-water were evaluated. A molecular dynamics simulation was performed to recognize molecular interaction between the compounds of the extracts with solvent molecules and to investigate the solvent molecules effect on photoluminescence spectra. The results were also compared with rhodamine 6G and we found that, at physiological pH (7.4), the fluorescent-coated silica nanoparticles obtained were also stable. We found that extraction solvents could be used for obtaining different nanomaterials for specific applications, and also found the best extraction solvent for obtaining EP nanomaterials for health care applications, specifically for imaging techniques.
Subject(s)
Biomarkers/analysis , Fabaceae/chemistry , Plant Extracts/chemistry , Solvents/chemistry , Ethanol/chemistry , Fabaceae/metabolism , Hydrogen-Ion Concentration , Methanol/chemistry , Nanoparticles/chemistry , Quantum Theory , Rhodamines/chemistry , Silicon Dioxide/chemistry , Spectrophotometry, Ultraviolet , Spectrum Analysis, Raman , Static Electricity , Water/chemistryABSTRACT
Despite the significant advances in the meniscus tissue engineering field, it is difficult to recreate the complex structure and organization of the collagenous matrix of the meniscus. In this work, we developed a meniscus prototype to be used as substitute or scaffold for the regeneration of the meniscal matrix, recreating the differential morphology of the meniscus by electrospinning. Synthetic biocompatible polymers were combined with the extracellular matrix component, collagen and used to replicate the meniscus. We studied the correlation between mechanical and structural properties of the polymer blend as a function of collagen concentration. Fibers were collected on a surface of a rapidly rotating precast mold, to accurately replicate each sectional morphology of the meniscus; different electro-tissues were produced. Detailed XRD analyses exhibited structural changes developed by electrospinning. We achieved to integrate all these electro-tissues to form a complete synthetic meniscus. Vascularization tests were performed to assess the potential use of our novel polymeric blend for promising meniscus regeneration.
