ABSTRACT
Colonization of sorghum and wheat after seed inoculation with Gluconacetobacter diazotrophicus strains PAL 5 and UAP 5541/pRGS561 (containing the marker gene gusA) was studied by colony counting and microscopic observation of plant tissues. Inoculum levels as low as 10(2) CFU per seed were enough for root colonization and further spreading in aerial tissues. Rhizoplane colonization was around 7 log CFU g(-1) (fresh weight). G. diazotrophicus was found inside sorghum and wheat roots with populations higher than 5 log CFU g(-1) (fresh weight). Stem colonization remained stable for 30 days post inoculation with endophyte concentrations from 4 to 5 log CFU g(-1) (fresh weight) (in both plants). Population in leaves decreased continuously being undetectable after 17 days post inoculation.
Subject(s)
Gluconacetobacter/physiology , Sorghum/growth & development , Sorghum/microbiology , Triticum/growth & development , Triticum/microbiology , Seeds/microbiology , Stem CellsABSTRACT
Between March and July 1996, a focalized epidemic outbreak of cutaneous leishmaniasis in General Vedia, province of Chaco, associated to the gallery forest of the Oro river was verified. The incidence rate in the area, which was 0-2/000 cases in preceding years, reached 8/000 cases in 1996. The risk of symptomatic infection was similar between sexes, but was different when analyzing the different age groups by sex, suggesting a greater relative importance of the peridomestic transmission for the females and of the transmission in the subtropical forest for the males. Specimens of Lutzomyia intermedia, a species already incriminated as a vector of Leishmaniasis in other provinces of northern Argentina, were captured and identified in the focus locality in May 1996. The possible causes of the outbreak related to the climatic variables and the vector abundance are analyzed and the results in the framework of possible preventive and control activities are discussed.
Subject(s)
Disease Outbreaks , Leishmaniasis, Cutaneous/epidemiology , Adolescent , Adult , Aged , Animals , Argentina/epidemiology , Child , Child, Preschool , Female , Humans , Incidence , Infant , Infant, Newborn , Insect Vectors , Male , Middle Aged , SeasonsABSTRACT
The response to recombinant 10-kD heat shock protein (HSP) of Mycobacterium leprae (rML10) was evaluated by indirect ELISA in sera from leprosy patients, household contacts, tuberculosis patients and healthy controls in a leprosy-endemic area in the North East of Argentina. Some technical parameters were analyzed: within-assay and between-assay variability, dose-response curves and detectability indexes (specificity and sensitivity) of ELISA applied to measure anti-10 kDa antibodies. High levels of these antibodies have already been reported in positive bacilloscopy patients; herein we have also demonstrated that tuberculosis patients sera cross-react with this M. leprae antigen. This test seems to have a low sensitivity and specificity for leprosy detection; it confirms that antibodies against highly conserved HSP antigens are important in the polyclonal response against mycobacterial epitopes in leprosy as well as in tuberculosis.
Subject(s)
Antibodies, Bacterial/immunology , Antigens, Bacterial/immunology , Chaperonin 10/immunology , Leprosy/immunology , Mycobacterium leprae/immunology , Tuberculosis/immunology , Adult , Aged , Aged, 80 and over , Cross Reactions , Humans , Middle Aged , Sensitivity and SpecificityABSTRACT
En el presente trabajo hemos controlado 30 pacientes con paracoccidioidomicosis, 17 de ellos fueron diagnosticados en el ultimo ano, todos varones, edad promedio de 44 anos al inicio de su enfermedad, con una demora promedio a la consulta de 8 meses.Predominan las formas clinicas mixtas (cutaneo-mucosa-pulmonar; cutaneo-mucosa y mucopulmonar) en un 84% de los casos; la mayoria provienen de areas rurales o suburbanas. El 85% fueron tratados con ketoconazol (derivado imidazolico con accion antifungica de amplio espectro) con muy buenos resultados. Se realizan controles clinicos, laboratoriales y serologicos periodicos para evaluar la mejoria, la regresion clinica de las lesiones precede a la negativizacion serologica y no hemos encontrado alterado el hepatograma de estos pacientes
Subject(s)
Adult , Middle Aged , Humans , Male , ParacoccidioidomycosisABSTRACT
En el presente trabajo hemos controlado 30 pacientes con paracoccidioidomicosis, 17 de ellos fueron diagnosticados en el ultimo ano, todos varones, edad promedio de 44 anos al inicio de su enfermedad, con una demora promedio a la consulta de 8 meses.Predominan las formas clinicas mixtas (cutaneo-mucosa-pulmonar; cutaneo-mucosa y mucopulmonar) en un 84% de los casos; la mayoria provienen de areas rurales o suburbanas. El 85% fueron tratados con ketoconazol (derivado imidazolico con accion antifungica de amplio espectro) con muy buenos resultados. Se realizan controles clinicos, laboratoriales y serologicos periodicos para evaluar la mejoria, la regresion clinica de las lesiones precede a la negativizacion serologica y no hemos encontrado alterado el hepatograma de estos pacientes
