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1.
Mol Cell ; 83(13): 2240-2257.e6, 2023 Jul 06.
Article in English | MEDLINE | ID: mdl-37329882

ABSTRACT

The RNA-binding ARS2 protein is centrally involved in both early RNA polymerase II (RNAPII) transcription termination and transcript decay. Despite its essential nature, the mechanisms by which ARS2 enacts these functions have remained unclear. Here, we show that a conserved basic domain of ARS2 binds a corresponding acidic-rich, short linear motif (SLiM) in the transcription restriction factor ZC3H4. This interaction recruits ZC3H4 to chromatin to elicit RNAPII termination, independent of other early termination pathways defined by the cleavage and polyadenylation (CPA) and Integrator (INT) complexes. We find that ZC3H4, in turn, forms a direct connection to the nuclear exosome targeting (NEXT) complex, hereby facilitating rapid degradation of the nascent RNA. Hence, ARS2 instructs the coupled transcription termination and degradation of the transcript onto which it is bound. This contrasts with ARS2 function at CPA-instructed termination sites where the protein exclusively partakes in RNA suppression via post-transcriptional decay.


Subject(s)
Nuclear Proteins , Transcription, Genetic , Nuclear Proteins/metabolism , Transcription Factors/metabolism , RNA Polymerase II/genetics , RNA Polymerase II/metabolism , RNA Stability/genetics , RNA-Binding Proteins/genetics , RNA-Binding Proteins/metabolism , RNA
2.
Mol Cell ; 81(3): 514-529.e6, 2021 02 04.
Article in English | MEDLINE | ID: mdl-33385327

ABSTRACT

Termination of RNA polymerase II (RNAPII) transcription in metazoans relies largely on the cleavage and polyadenylation (CPA) and integrator (INT) complexes originally found to act at the ends of protein-coding and small nuclear RNA (snRNA) genes, respectively. Here, we monitor CPA- and INT-dependent termination activities genome-wide, including at thousands of previously unannotated transcription units (TUs), producing unstable RNA. We verify the global activity of CPA occurring at pA sites indiscriminately of their positioning relative to the TU promoter. We also identify a global activity of INT, which is largely sequence-independent and restricted to a ~3-kb promoter-proximal region. Our analyses suggest two functions of genome-wide INT activity: it dampens transcriptional output from weak promoters, and it provides quality control of RNAPII complexes that are unfavorably configured for transcriptional elongation. We suggest that the function of INT in stable snRNA production is an exception from its general cellular role, the attenuation of non-productive transcription.


Subject(s)
Cleavage And Polyadenylation Specificity Factor/metabolism , DNA-Binding Proteins/metabolism , RNA Polymerase II/metabolism , RNA, Small Nuclear/biosynthesis , Transcription Termination, Genetic , Cleavage And Polyadenylation Specificity Factor/genetics , DNA-Binding Proteins/genetics , HeLa Cells , Humans , Polyadenylation , Promoter Regions, Genetic , RNA Polymerase II/genetics , RNA, Small Nuclear/genetics
3.
J Vis Exp ; (135)2018 05 03.
Article in English | MEDLINE | ID: mdl-29782024

ABSTRACT

When steady state RNA levels are compared between two conditions, it is not possible to distinguish whether changes are caused by alterations in production or degradation of RNA. This protocol describes a method for measurement of RNA production, using 5-Bromouridine labelling of RNA followed by immunoprecipitation, which enables investigation of RNA synthesized within a short timeframe (e.g., 1 h). The advantage of 5-Bromouridine-labelling and immunoprecipitation over the use of toxic transcriptional inhibitors, such as α-amanitin and actinomycin D, is that there are no or very low effects on cell viability during short-term use. However, because 5-Bromouridine-immunoprecipitation only captures RNA produced within the short labelling time, slowly produced as well as rapidly degraded RNA can be difficult to measure by this method. The 5-Bromouridine-labelled RNA captured by 5-Bromouridine-immunoprecipitation can be analyzed by reverse transcription, quantitative polymerase chain reaction, and next generation sequencing. All types of RNA can be investigated, and the method is not limited to measuring mRNA as is presented in this example.


Subject(s)
Immunoprecipitation/methods , Polymerase Chain Reaction/methods , RNA/chemical synthesis , Uridine/analogs & derivatives , Bromouracil/analogs & derivatives , Uridine/chemistry
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