The COVID-19 wave was already here: High seroprevalence of SARS-CoV-2 antibodies among staff and students in a Cameroon University.

Background: Seroprevalence studies, to estimate the proportion of people that has been infected by SARS-CoV-2 are importance in African countries, where incidence is among the lowest in the world. Objective: This study aimed at evaluating the exposure to SARS-CoV-2 within a university setting of Cameroon. Methods: A cross-sectional study performed in December 2020 - December 2021, among students and staffs of the Evangelical University of Cameroon. COVID-19 antigen rapid detection test (RDT) was performed using Standard Q Biosensor, and one year after SARS-CoV-2 antibody-test was performed within the same population using RDT and chemiluminescence immunoassay (CLIA). Results: 106 participants were enrolled (80% students), female sex was the most represented. Positivity to SARS-CoV-2 was 0.0% based on antigen RDTs. The seroprevalence of SARSCoV- 2 antibodies was estimated at 73.6% (95% CI. 64.5-81.0) for IgG and 1.9% (95% CI. 0.2-6.8) for IgM/IgG with RDTs, and 91.9% (95% CI. 84.7-96.4) for anti-nucleocapsid with CLIA. 95.3% (101) reported having developed at least one of the known COVID-19 symptoms (cough and headache being the most common). 90.3% (28) of people who experienced at least one of these symptoms developed IgG antibodies. 40.6% (43) of participants took natural herbs, whereas 55.7% (59) took conventional drugs. The most used herb was Zingiber officinale, while the most used drugs were antibiotics. Conclusion: In this Cameroonian University community, SARS-CoV-2 seroprevalence is high, with a greater detection using advanced serological assays. This indicates a wide viral exposure, and the need to adequate control measures especially for those experiencing any related COVID-19 symptoms.

The edible plant Amaranthus hybridus (Amaranthaceae) prevents the biochemical, histopathological and fertility impairments in colibri®-treated female rats.

Colibri® a commercial formulation of Imidacloprid severely impairs the reproductive function. This study aimed at evaluating the preventive effects of Amaranthus hybridus on the reproductive toxicity of colibri® in female rats. Eighty rats (n = 10/group) were orally treated with colibri® (22.5 mg/kg) and co-administered with either aqueous or methanolic extracts of A. hybridus (55 or 110 mg/kg) within four weeks. Control animals received either distilled water (10 ml/kg), clomiphene citrate or vitamin E. Starting from day 18 of treatment till the end, half of animals in each group (n = 5) was used for the fertility test whereas the remaining rats were kept under treatment until sacrifice. Blood, ovaries, uterus and vagina were collected after sacrifice for measurement of sexual hormones, oxidative stress markers and histological assessment. Exposure of female rats to colibri® was followed by a significant reduction (p < 0.05) in the ovarian and uterine weights, LH, FSH, estradiol and progesterone levels as well as ovarian superoxide dismutase, catalase and peroxidase activities. Moreover, alteration of ovaries, uteri and vagina histology, increase in MDA concentration, decrease in fertility and parturition indices and, pup's viability were recorded. Co-administration of colibri® and plant extracts significantly (p < 0.05-0.001) prevented the above-mentioned damages through biochemical parameter regulations. These results suggest that A. hybridus exerts a preventive effect against colibri®-induced female reproductive toxicity.

Dracaena arborea (Dracaenaceae) Increases Sexual Hormones and Sperm Parameters, Lowers Oxidative Stress, and Ameliorates Testicular Architecture in Rats with 3 Weeks of Experimental Varicocele.

Varicocele is a disease characterized by an abnormal dilation of the pampiniform plexus that drains the testis. The main objective of this work was to evaluate the curative effects of aqueous and ethanolic extracts of Dracaena arborea on some reproductive and antioxidant markers in rats with experimental varicocele. Following varicocele induction, rats (5 per group) were randomly partitioned into untreated varicocele, vitamin E-treated (150 mg/kg), aqueous extract-treated (500 mg/kg), and ethanolic extract-treated (100 mg/kg) animals. Two other groups served as normal and sham-operated. After 2 or 4 weeks of treatments, body and sex organ weights, spermatozoa characteristics, antioxidant status, NO level, sex hormones, and testis histology were measured. Animals with 3 weeks of varicocele showed a significant (p < 0.05-0.001) decrease in body and sex organ weights, total proteins, sperm characteristics, testosterone concentration, SOD, catalase, and total peroxidase activities. An increase in the plasmatic FSH, LH, and testicular MDA and NO concentrations was also recorded. Moreover, marked disorganization of the testicular architecture was observed. Treatment with D. arborea significantly reversed these impairments due to varicocele. For instance, after 4 weeks, treatment with aqueous extract of D. arborea significantly (p < 0.05-0.001) increased testes and epididymis weights, sperm viability (89.12 ± 1.09 vs 68.22 ± 1.42), sperm density (148.50 ± 2.59 vs 110.25 ± 2.51), and sperm motility (68.16 ± 2.39 vs 55.88 ± 3.20) in the left side, compared with varicocele-untreated rats. The extract also significantly (p < 0.05-0.001) decreased malondialdehyde level (2.19 ± 0.04 vs 3.50 ± 0.13) but elevated catalase (0.97 ± 0.03 vs 0.55 ± 0.03), SOD (0.5 ± 0.03 vs 0.15 ± 0.03), and peroxidase (65.80 ± 2.9 vs 40.95 ± 2.44) activities. Present results showed that D. arborea extracts possess antioxidant effects and improve sperm quality in male rats with an existing varicocele.

Uterotonic Effects of Aqueous and Methanolic Extracts of Lannea acida in Wistar Rats: An In Vitro Study.

Lannea acida (Anacardiaceae), commonly called Kikié in the Noun division (West-Cameroon), is a tree whose bark is used locally to facilitate delivery. This study was aimed at evaluating the in vitro uterotonic effects of aqueous and methanol extracts of L. acida in Wistar rats. Uterine strips isolated from rats pretreated with 5 µg estradiol (48 h) were mounted in a single-organ bath containing aerated and thermostated De Jalon solution (37 °C). After equilibration, non-cumulative effects of L. acida extracts were recorded after application. The effect of the methanol extract (the most active extract) was monitored in the presence of atosiban (a competitive antagonist of oxytocin receptors), atropine (a specific type 3 muscarinic receptor antagonist), nifedipine (an L-type calcium channel antagonist), and 2-Aminoethoxydiphenyl borate (2-ADB, a specific antagonist of inositol 1,4,5-triphosphate receptors type 1), and in calcium-free medium containing EGTA to elucidate its mechanism of action. L. acida induced uterine contraction in a concentration-dependent manner with the methanol extract (1.506 ± 0.032 gf) being the most effective. Administration of atosiban (2 µmol/L) and atropine (1 µmol/L) reduced the contractile effect of L. acida. Complete inhibition was observed with nifedipine, 2-APB, and calcium-free medium containing EGTA. These results suggest that L. acida possesses uterotonic effects mediated through oxytocin receptors with mobilization of extracellular calcium.

Rubus apetalus (Rosaceae) improves spermatozoa characteristics, antioxidant enzymes and fertility potential in unilateral cryptorchid rats.

BACKGROUND: Cryptorchidism (CPT) is an important cause of male infertility. Rubus apetalus is a medicinal plant with a powerful antioxidant potential. We investigated the effects of aqueous and methanolic extracts of R. apetalus on spermatozoa parameters, antioxidant enzymes and fertility potential of rats with experimental unilateral CPT. METHOD: Normal (n = 15), sham-operated (n = 15) and cryptorchid rats (n = 80; distributed into 16 groups of 5 rats/group) were treated for 2, 4 or 8 weeks with either distilled water (10 ml/kg/day), vitamin E (75 mg/kg/day), aqueous or methanolic extract of R. apetalus (12 and 60 mg/kg). Sex organ weights, spermatozoa parameters, testicular proteins, sex hormones, fertility potential, morphometric characteristics of testis and oxidative stress markers were measured. RESULTS: CPT significantly (p < 0.05-0.001) decreased testicular and epididymal weights, spermatozoa density, spermatozoa motility, spermatozoa normality, testicular proteins, LH, FSH and testosterone concentrations. In cryptorchid rats, peri-vascular fibrosis significantly increased (p < 0.001), while diameter of the seminiferous tube, germ cell thickness, gestation index and fertility index decreased when compared to control. Additionally, CPT induced oxidative stress by increasing lipid peroxidation and by reducing superoxide dismutase and catalase activities. These alterations were corrected by R. apetalus. For instance, a significantly increase (p < 0.05-0.001) in spermatozoa motility, normality, viability and density after 2, 4 and 8 weeks of treatment was noticed. R. apetalus also increased (p < 0.05-0.001) testicular proteins, gestation index (90-100%) and fertility index (90-100%), compared to the untreated cryptorchid rats. CONCLUSION: R. apetalus boosts fertility potential in cryptorchid rats and could be considered as a promising alternative agent for the management of infertility associated with CPT.

CONTEXTE ET JUSTIFICATIF: La cryptorchidie (CPT) est. une importante cause de l'infertilité masculine dans le monde. Rubus apetalus est. une plante médicinale au potentiel antioxydant avéré. L'objectif de ce travail était d'évaluer les effets des extraits aqueux et méthanolique de R. apetalus sur les paramètres spermatiques, les enzymes antioxydantes et le potentiel de fertilité des rats à CPT unilatérale. MÉTHODE: Les rats normaux (n = 15), simulés (n = 15) et cryptorchides (n = 80; répartis en 16 groupes de 5 rats/groupe) ont été traités pendant 2, 4 ou 8 semaines avec de l'eau distillée (10 ml/kg/jour), la vitamine E (75 mg/kg/jour) ou l'extrait aqueux ou méthanolique de R. apetalus (12 et 60 mg/kg). La masse des organes sexuels, les caractéristiques spermatiques, les marqueurs du stress oxydatif, les hormones sexuelles, l'histomorphométrie testiculaire et la fertilité ont été mesurés au terme des traitements. RÉSULTATS: La CPT a significativement diminué (p < 0,05-0,001) la masse testiculaire et épididymaire du côté cryptorchide. Le même constat était fait par rapport à la densité, à la motilité, à la normalité spermatique ainsi que les protéines testiculaires et les hormones sexuelles (LH, FSH et testostérone plasmatiques). Chez les rats cryptorchides, la fibrose péri-vasculaire a augmenté de manière significative (p < 0,001) tandis que le diamètre des tubes séminifères, l'épaisseur des cellules germinales, l'indice de gestation et l'indice de fertilité ont diminué par rapport au témoin. De plus, le CPT a induit un stress oxydatif caractérisé par la peroxydation lipidique et la réduction de l'activité des enzymes antioxydantes (SOD et catalase). Ces altérations ont été corrigées par R. apetalus. Une augmentation significative (p < 0,05-0,001) des paramètres spermatiques, des protéines testiculaires et des hormones sexuelles a été notée après 2, 4 et 8 semaines de traitement aux extraits de plante. R. apetalus a par ailleurs normalisé l'activité des enzymes antioxydantes et augmenté l'indice de gestation (90­100%) et l'indice de fertilité (90­100%) par rapport aux rats cryptorchides non traités. CONCLUSION: R. apetalus augmente le potentiel de fertilité chez les rats cryptorchides et pourrait être une alternative prometteuse pour la prise en charge de l'infertilité masculine due à la cryptorchidie.

Prevalence and factors associated with dysmenorrhea in women at child bearing age in the Dschang Health District, West-Cameroon.

INTRODUCTION: dysmenorrhea is a painful phenomenon at the pelvis region preceding or following menstruation. Dysmenorrhea accounts among the most frequent problem of women at child bearing age and affects 45% to 95% of them. According to the WHO, 16.8 to 81% of women are affected by dysmenorrhea. The present study was carried out at the Dschang Health District in order to determine the prevalence of dysmenorrhea and associated factors among women at child bearing age. METHODS: a transversal community-based study was carried out from March to June 2018. Information regarding socio-demographic features, prevalence, factors associated with the dysmenorrhea and the effect of dysmenorrhea on daily activities were collected using structured questionnaire and data were analyzed using Epi Info version 7.1.3.3 Software. RESULTS: a total of 637 women aged 12 to 50 years were interviewed in the present study. The mean body mass index was 25.94 with an average weight of 66.41 kilogram. Fifty six point twenty percent (56.20%) of participants had dysmenorrhea. From all risks factors fund only the normal body mass index (OR = 3.08, P-value = 0.01) having a significant association with the occurrence of dysmenorrhea. Daily activities were affected in 73.25% of participants dysmenorrheic and those who had some episodes of dysmenorrhea. CONCLUSION: the present study showed that more than a half of respondents were dysmenorrheic and several factors were associated with this pathology. This study also suggests that dysmenorrhea have a negative impact on the daily activities of women at child bearing age.