Subject(s)
Feces/microbiology , Gastroenteritis/etiology , Virus Diseases/diagnosis , Child , Enzyme-Linked Immunosorbent Assay , Humans , Methods , RotavirusABSTRACT
In a year's survey (December 1976-November 1977) of 190 children admitted to hospital with acute gastroenteritis, an "aetiological" agent was found in stools by electron microscopy in 41% of cases. Rotavirus were found in 34% of patients. We also found Coronavirus-like particles and Astrovirus-like particles in 25 and 5 stools, respectively. Improvements are awaited to demonstrate the responsibility of these virus in gastroenteritis.
Subject(s)
Coronaviridae/isolation & purification , Feces/microbiology , Gastroenteritis/microbiology , Mamastrovirus/isolation & purification , RNA Viruses/isolation & purification , Rotavirus/isolation & purification , Viruses, Unclassified/isolation & purification , Acute Disease , Child , Gastroenteritis/etiology , HumansABSTRACT
A simple detection method of enteroviruses and adenoviruses in drinking water has been performed, using adsorption-elution on to cellulose nitrate membranes. Average of enteroviruses recovering is varying from 23 to 80% when 30 liters of water containing 60 PFU are monitored. Only 10% of adenoviruses are recovered in the same conditions. This method allowed us to detect 12 PFU of enteroviruses in 120 liters of water. Recovering of 1 PFU in 10 liters when examining more than 100 liters is reproducible.