Sensing of azo toxic dyes using nanomaterials and its health effects - A review.

Development of science has taken over our lives and made it mandatory to live with science. Synthetic technology takes more than it has given for our welfare. In the process of meeting the demand of the consumers, industries supported synthetic products to meet the same. One such sector that employs synthetic azo dyes for food coloring is the food industry. The result of the process is the production of a variety of colored foods which looks more appealing and palatable. The process not only meets the consumer's demand it also has an impact on customers' health because the consumption of azo-toxic dye-treated foods regularly or in direct contact with synthetic azo dyes can also cause severe human health consequences. Nanotechnology is a rapidly evolving branch of research in which nanosensors are being developed for a variety of applications, including sensing various azo-toxic dyes in food products, which provides a wider scope in the future, with the innovation in designing different nanosensors. The current review focuses on the different types of nanosensors, their key role in sensing, and the sensing of azo toxic dyes using nanosensors, their advantages over other sensors, applications of nanomaterials, and the health impacts of azo dyes on humans, appropriate parameters for maximum permissible limits, and an Acceptable Daily Intake (ADI) of azo toxic dye to be followed. The regulations followed on the application of colorants to the food are also elaborated. The review also focuses on the application of enzyme-based biosensors in detecting azo dyes in food products.

Carbon nanomaterials and its applications in pharmaceuticals: A brief review.

Nanotechnology for the past decade has made tremendous improvement and diverse applications in various sector. Among the nanomaterials synthesized, carbon allotropes are advantageous due to its easy functionalization, conductivity, surface area and electrical activity. Hence, they are termed as "Wonder materials". Allotropes such as carbon nanotubes, graphene, graphene oxide, fullerens, and carbon dots has paved its importance in the pharmaceuticals. They are coated in the biomedical devices, applied in the therapeutics and diagnosis. These are also used in the treatment of cancer and they possess anti-microbial and antiviral activity. Carbon nanomaterials possess several applications from biosensors to remediation of pollutants. Detection of hazardous compounds in food, pharmaceutical products, gene and drug delivery. They are also used in tissue regeneration and gene therapy. Application of carbon allotropes in the current scenario provides a wide scope in future with improvisations in building electrochemical biosensors. Its selectivity, sensitivity and cost-effectiveness prove it to be better alternative compared to other nanomaterials. The review focuses on the carbon allotropes used in pharmaceuticals, biosensors, pollutants detection and treatment were discussed in detail.

Production, purification and application of Cutinase in enzymatic scouring of cotton fabric isolated from Acinetobacter baumannii AU10.

Conventional cotton scouring in the textile industry using alkali results in huge environmental impact which can be overcome by using enzymes. Pectinase along with cutinase gives enhanced bioscouring results. Cutin was extracted from tomato peels and was used as substrate in the microbial media. The strain isolated from tomato peel was identified as Acinetobacter baumannii AU10 by 16S rDNA sequencing. The cutinase production was optimized by Placket-Burman and Response Surface Methodology (RSM) and the maximum production of 82.75 U/mL obtained at sucrose 6.68% (w/v), gelatin 2.74 g/L at a temperature of 35.93 °C. Cutinase was purified by ammonium sulfate precipitation, hydrophobic interaction chromatography and ion exchange chromatography with a recovery of 25.6% and specific activity of 38030 U/mg. The confirmation test for the purity of cutinase was analyzed by RP-HPLC. The molecular mass of cutinase was determined as 28.9 kDa by SDS-PAGE technique. Scanning electron microscopic analysis showed a rough and open primary wall surface on the cutinase bioscoured fabric which confirmed its activity on cutin present in the cotton fabric. Additionally, the cutinase-bioscoured samples showed better absorbency than the untreated samples. Therefore, enzymatic scouring increases wetting capacity of scoured cotton and also helps to reduce environmental pollution.

Emodin and Its Role in Chronic Diseases.

Diseases, such as heart disease, stroke, cancer, respiratory diseases, and diabetes, are by far the leading cause of mortality in the world, representing 60 % of all deaths. Although substantial medical advances have been made and many therapeutic approaches proposed yet traditional medicine and medicinal plants find an important place in therapy. They have been providing invaluable solutions to the various health problems. Emodin (1,3,8-trihydroxy-6-methylanthraquinone) is a natural anthraquinone derivative found in various Chinese medicinal herbs. Traditionally, it has been used as an active constituent of many herbal laxatives. However, in the last few years, significant progress has been made in studying the biological effects of emodin at cellular and molecular levels and it is emerging as an important therapeutic agent. This review provides an overview of the modulatory effects of emodin in various diseases and cell signaling pathways, which may have important implications in its future clinical use.

Accessory left testicular artery in association with double renal vessels: a rare anomaly.

A rare association of accessory testicular artery along with double renal arteries and accessory renal vein was observed unilaterally on the left side. In addition, the left inferior phrenic artery was arising from the left gastric branch of the coeliac trunk. This was observed in the dissection of an adult male cadaver during a routine undergraduate teaching programme. In this case, the accessory left testicular artery originated superior to the normal testicular artery from the descending abdominal aorta immediately below the origin of the normal left renal artery. In addition to this artery, a variant renal artery was noted with three segmental branches before entering the hilum. The accessory renal vein emerged from the lower pole after the receiving testicular vein joined the main renal vein. The left inferior phrenic artery arose from the left gastric branch of the coeliac trunk. An anatomical description of this uncommon variation is presented in this case report, highlighting its clinical implications.