ABSTRACT
The antibiotic anisomycin inhibits protein synthesis, which much research has suggested is required for the formation of long-term memory. The present work studied the effects of acute subcutaneous administration of anisomycin on the consolidation of memory in an inhibitory avoidance task in CD1 mice of both sexes. The animals were separated by sex and randomly distributed into three groups: two groups were injected with 150 mg/kg anisomycin, one immediately after the training phase and the other 24 h later, while the control group received saline. The interval between training and test was four days. Anisomycin administrated immediately after training produced statistically significant impairment of memory, which was not observed when the drug was administered 24 h after training. No sex differences were observed in the effects of anisomycin. These results extend to female mice the memory impairing effects of anisomycin previously observed in males and endorse the hypothesis that the establishment of long-term memory depends on protein synthesis shortly after training.
Subject(s)
Anisomycin/pharmacology , Avoidance Learning/drug effects , Neural Inhibition/drug effects , Protein Synthesis Inhibitors/pharmacology , Animals , Anisomycin/administration & dosage , Female , Male , Memory/drug effects , Mice , Protein Synthesis Inhibitors/administration & dosageABSTRACT
The antibiotic anisomycin inhibits protein synthesis, which much research has suggested is required for the formation of long-term memory. The present work studied the effects of acute subcutaneous administration of anisomycin on the consolidation of memory in an inhibitory avoidance task in CD1 mice of both sexes. The animals were separated by sex and randomly distributed into three groups: two groups were injected with 150 mg/kg anisomycin, one immediately after the training phase and the other 24 h later, while the control group received saline. The interval between training and test was four days. Anisomycin administrated immediately after training produced statistically significant impairment of memory, which was not observed when the drug was administered 24 h after training. No sex differences were observed in the effects of anisomycin. These results extend to female mice the memory impairing effects of anisomycin previously observed in males and endorse the hypothesis that the establishment of long-term memory depends on protein synthesis shortly after training (AU)
El antibiótico anisomicina inhibe la síntesis de proteínas, la cual muchos estudios indican que es necesaria para la formación de la memoria a largo plazo. En el presente trabajo se estudiaron los efectos de la administración aguda subcutánea de anisomicina sobre la consolidación de la memoria en una tarea de evitación inhibitoria en ratones CD1 de ambos sexos. Los animales fueron separados por sexo y distribuidos al azar en tres grupos: dos grupos fueron inyectados con 150 mg/kg de anisomicina, uno inmediatamente después de la fase de entrenamiento y el otro 24 h después, mientras que el grupo control recibió suero salino. El intervalo entre el entrenamiento y el test fue de cuatro días. La anisomicina administrada inmediatamente después del entrenamiento produjo un deterioro de memoria estadísticamente significativo, deterioro que no fue observado cuando el fármaco fue administrado 24 h después del entrenamiento. No se observaron diferencias de sexo en los efectos de la anisomicina. Estos resultados extienden a las hembras los efectos deteriorantes de la anisomicina sobre la memoria previamente observados en machos y respaldan la hipótesis de que el establecimiento de la memoria a largo plazo depende de la síntesis de proteínas poco después del entrenamiento (AU)
