Infecção de sítio cirúrgico em revascularizações do miocárdio: estudo retrospectivo / Surgical site infection in myocardial revascularization: a retrospective study

Objetivo: analisar a incidência de infecção do sítio cirúrgico em revascularizações do miocárdio. Métodos: estudo quantitativo com 314 prontuários de pacientes submetidos às revascularizações no período de 2014 a 2016, do serviço de cirurgia cardíaca. Foram avaliadas as relações de variáveis operatórias com o aparecimento de infecção de sítio cirúrgico para determinar os fatores significativos. Para a coleta dos dados utilizou-se as fichas de circulação extracorpórea e para a análise as definições estabelecidas pela Agência Nacional de Vigilância Sanitária. Resultados: 66,2% eram do sexo masculino; 62,1%, brancos; 47,3%, hipertensos; 22,8%, diabéticos e 91,7%, cirurgias eletivas. Evidenciou-se que febre, edema local em 24 horas, dor, necrose em lesão operatória e (re)abordagem cirúrgica são os principais fatores para incidência de infecções relacionada ao tempo médio de circulação extracorpórea e de cirurgia. Conclusão: a infecção de sítio cirúrgico em revascularizações está relacionada com o tempo de cirurgia e de circulação extracorpórea.

Objective: to analyze the incidence of surgical site infection in myocardial revascularizations.Methods: a quantitative study with 314 medical records of patients submitted to revascularizationin the period from 2014 to 2016, of the cardiac surgery service. The relationships of operativevariables with the appearance of surgical site infection were evaluated to determine the significantfactors. Data collection was done using the cardiopulmonary bypass records and for the analysis thedefinitions established by the National Sanitary Surveillance Agency. Results: 66,2% were male;62,1% white; 47,3% hypertensive and 22,8% diabetic; 91.7% were elective surgeries. It was evidencedthat fever, local edema in 24 hours, pain, necrosis in operative lesion and surgical intervention are the main factors for the incidence of infections related to the mean time of extracorporealcirculation and surgery. Conclusion: the surgical site infection in revascularizations is related to thetime of surgery and extracorporeal circulation.

The surgical management of extra-articular ankylosis in noma patients.

Extraarticular ankylosis occurs frequently in children suffering from noma sequelae. Over 20years of operating on these patients, we observed high recurrence of mouth opening limitation. We therefore progressively changed our surgical strategy. This retrospective study compares the impact of different parameters (types of surgery, noma type, physiotherapy compliance, age and sex) on immediate and long-term mouth opening. It includes a series of 121 patients with extraarticular ankylosis operated on between 1990 and 2015. Soft tissue reconstruction evolved from local and pedicled flaps to large free flaps. Mouth opening was performed by bone-bridge excision, sometimes associated to contralateral coronoidectomy. Mouth opening technique including bilateral coronoidectomy with free flap reconstruction was the only independent factor for significantly better immediate mouth opening with a mean increase of 8.7mm [95% confidence interval (CI) 4.3-13.1, P<0.001) and this effect was maintained in the 3years of follow-up. Another positive factor related to long-term results was excellent physiotherapy, while noma type 4 was a negative factor. Recurrence remains problematic in the management of noma sequelae. If physiotherapy and long-term follow-up cannot be offered, patients should not be operated on, because if limitation of mouth opening recurs, oral feeding may become impossible when a facial defect has been reconstructed.

[Orbitotemporal facial involvement in type 1 neurofibromatosis (NF1)]. / L'atteinte orbito-temporo-faciale dans la neurofibromatose de type 1 (NF1).

Plexiform neurofibromas of the orbit, sometimes extending to the temporal region and the face, are considered to be a rare but devastating and disfiguring complication of neurofibromatosis type 1. The first symptoms appear in infancy and the involvement of the orbit and the face is present in nearly all children after the age of 5. The disease is unilateral in most cases but can exceptionally involve both sides of the face. Progressive deformation of the orbital frame due to the expanding plexiform neurofibroma and buphthalmos occurs in a large proportion of cases. The associated sphenoidal dysplasia, which is thought to be, according to the most recent hypothesis, genetically determined, will inescapably increase the burden to the orbital content, cause pulsating proptosis and will endanger noble structures, finally resulting in loss of vision. Using the Jackson classification, the authors report their personal series of 22 cases (19 operated). Until now, there has been no effective medical treatment for plexiform neurofibroma and surgery remains the standard care for these patients. Controversies remain about the timing of the first operation and today most multidisciplinary teams involving plastic, maxillofacial, ophthalmologic, and neurosurgeons favor early intervention to try to minimize the secondary deformation of the orbital and facial skeleton. A number of cases of plexiform neurofibromas are illustrated within the three Jackson groups and treatment results of the rare elephantiasis neuromatosa cases are presented. Special techniques such as preoperative embolization of heavily vascularized plexiform neurofibroma are also discussed.

[About 10 cases of Launois-Bensaude disease]. / La maladie de Launois-Bensaude : description, prise en charge. A propos de dix patients opérés.

Launois-Bensaude syndrome is a rare pathology. The presence of multiple, symmetric, non encapsulated lipomatous masses in the face, neck, upper arms and upper trunk is typical. Men are especially affected between age of 35 and 50. The disease is frequently associated with alcoholism, hepatopathy, glucose intolerance, hyperuricemia, and malignant tumors of the upper airways, requiring thorough clinical evaluation of all patients. Cosmetic deformity and compression lead patients to seek treatment. Therapy is difficult. Dietary treatment and weight loss are of limited value in the management of this pathology. Surgery is the only effective treatment. The surgeon must make sure that the patient has ceased his alcohol abuse before performing this treatment. Conventional surgery or liposuction are used to restore a normal social life to these patients, deformed by their disease. This surgical management has a severe tendency to haemorrhage, dissection is complicated by infiltrations of neighbouring tissue and is frequently followed by recurrence, mainly after liposuction. Nevertheless, based on a review of ten cases, good results of surgical removal must prompt to operate them on.

[Humanitarian plastic surgery in question]. / La chirurgie plastique humanitaire en question.

Humanitarian plastic surgery has become very fashionable and more and more surgeons are attracted by this type of commitment. The authors remind here of the necessary conditions and limitations of these actions. The communicative action according to J. Habermas, which means a true partnership with the local health care specialists should be the only valid engagement.

[GESNOMA (Geneva Study Group on Noma): state-of-the-art medical research for humanitarian purposes]. / GESNOMA (Geneva Study group on Noma): une recherche médicale de pointe à but humanitaire.

Noma is a devastating gangrenous disease that leads to severe tissue destructions in the face. It is seen almost exclusively in children living in less developed countries. The exact prevalence of the disease is unknown and the cause remains unknown too. Risk factors are: malnutrition, a compromised immune system, poor oral hygiene and a lesion of the gingival mucosal barrier, as well as an unidentified bacterial factor. Herpes viruses might also contribute. Studies of the buccal flora in acute phases of noma and comparison with control children do not exist. Our study takes place in Niger. For each child (cases and controls) we take samples of gingival fluid, saliva, blood and mouth mucosal swabs. The samples are analysed in Geneva in different laboratories. We control the serologies for Herpes viruses and measles. We also perform a nutritional assessment and the mucosal swabs are cultivated for the presence of viruses. The gingival flora is investigated by microarrays. These microarrays are instrumental to test for the presence of thousands of different bacteria in each clinical sample. This method allows a qualitative and quantitative description of the oral flora in noma-children and control cases. This is the first large scale study on the etiology of noma which uses new technical approaches for humanitarian purposes.

[Lip reconstruction in noma sequelae]. / Reconstruction labiale dans les séquelles de noma.

Noma is a grangenous stomatitis which might extend to other facial structures leading to extensive soft tissue and bony defects. Reconstruction of noma sequellae should take into account a few principles in relation to the particular aspect of this disease: (1) correction of the mandibular constriction; (2) removal of scar tissue in order to recreate the initial defect; (3) reconstruction of the missing bony framework; (4) reconstruction of the maxilla and upper lip before building the nose in case it is destroyed; (5) anticipation of the facial growth. Reconstruction of the lips, in case of extensive defects often necessitates a distant skin flap. As free flaps, the forearm fascio-cutaneous flap or the serratus musculo-cutaneous flap were used according to the size of the defect. The mucosal part of the lip is usually reconstructed with a local flap from the adjacent lip or an heterolabial flap (Estlander flap).

[Labial expansion]. / Expansion labiale.

Lip expansion is an old procedure used by some ethnic groups to follow traditions of their ancestry. Medically speaking since the beginning of the XIXth century, various devices have been designed for distending the angles of the mouth in order to recreate the normal width of the mouth aperture. In the recent years, thanks to the development and refinements of new flaps, expanding the lips is rarely necessary. In proper indications however, specially in the vermillon border, lip expansion remains a good indication.

Barium sulphate and soft-tissue radiology: allying the old and the new for the investigation of animal cutaneous microcirculation.

The study of microcirculation using angiography is essential to the advancement of flap and angiogenesis research in plastic surgery. Until the mid-1980s, barium sulphate was the most commonly used contrast material, although it did not provide optimal visualisation of the vascular tree. In 1986, a new technique using lead oxide was proposed, which permitted very high-quality imaging and rapidly became the technique of choice, despite its high toxicity. We reconsider the former technique of barium-sulphate injection and combine it with soft-tissue radiology using mammographic film to achieve a radiological definition similar to that obtained with lead oxide, and discuss the advantages and disadvantages of the two methods. We conclude that barium sulphate and the use of mammographic film is an accurate, simple and non-toxic method of analysing the cutaneous circulation in small animals.

[Correction of cervical contractures after burns with pedicled latissimus dorsi musculocutaneous flaps]. / Correction des rétractions cervicales après brûlures par lambeau musculocutané pédiculé du grand dorsal.

In selected cases of cervical retractions after burns, the authors show that pedicled latissimus dorsi musculocutaneous flaps may be of great help if properly performed. The main advantages of this method are its simplicity and the lack of need for a postoperative cervical splint.

Clinical experience in the treatment of noma sequelae.

We report a cohort of 148 African children operated on between 1985 and 2000 for noma sequelae. A total of 440 operations were performed. Lesions included mouth constriction in 70 of 148 cases, and severe bone destruction in 69 of 148 cases. A large proportion of children was transferred to Switzerland for surgical treatment, whereas the others were operated on in local hospitals in Africa. Vascularized calvarium flap was mostly used for bone reconstruction (n = 36). Pedicled latissimus dorsi myocutaneous flap was the preferred strategy for cheek reconstruction (n = 40). Expanded frontal flap was used for nasal reconstruction (n = 18), and pedicled heterolabial flap for lip construction (n = 37). Given the elevated level of recurrence of mouth constriction (extraarticular ankylosis), a minimum of 2 years' follow-up after surgical treatment was considered mandatory. Follow-up was conducted by field nurses from the humanitarian organizations, and a third of the patients were seen directly by our surgical team during special missions to Africa.

Delivery of FGF-2 but not VEGF by encapsulated genetically engineered myoblasts improves survival and vascularization in a model of acute skin flap ischemia.

Stimulating angiogenesis by gene transfer approaches offers the hope of treating tissue ischemia which is untreatable by currently practiced techniques of vessel grafting and bypass surgery. Vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (FGF-2) are potent angiogenic molecules, making them ideal candidates for novel gene transfer protocols designed to promote new blood vessel growth. In this study, an ex vivo gene therapy approach utilizing cell encapsulation was employed to deliver VEGF and FGF-2 in a continuous and localized manner. C(2)C(12) myoblasts were genetically engineered to secrete VEGF(121), VEGF(165) and FGF-2. These cell lines were encapsulated in hollow microporous polymer membranes for transplantation in vivo. Therapeutic efficacy was evaluated in a model of acute skin flap ischemia. Capsules were positioned under the distal, ischemic region of the flap. Control flaps showed 50% necrosis at 1 week. Capsules releasing either form of VEGF had no effect on flap survival, but induced a modest increase in distal vascular supply. Delivery of FGF-2 significantly improved flap survival, reducing necrosis to 34.2% (P < 0.001). Flap vascularization was significantly increased by FGF-2 (P < 0.01), with numerous vessels, many of which had a large lumen diameter, growing in the proximity of the implanted capsules. These results demonstrate that FGF-2, delivered from encapsulated cells, is more efficacious than either VEGF(121) or VEGF(165) in treating acute skin ischemia and improving skin flap survival. Furthermore, these data attest to the applicability of cell encapsulation for the delivery of angiogenic factors for the treatment and prevention of tissue ischemia.

[Humanitarian plastic surgery. Personal experience and reflections]. / Chirurgie plastique humanitaire. Expérience personnelle et réflexions.

After analysing their concept of humanitarian plastic surgery, the authors present their personal experience which started in the 1970s, concerning two aspects: in the field (Burkina-Faso, Mali, Niger) and in Geneva, Switzerland, where the more difficult cases are operated. They illustrate their approach by a clinical case of sequelae of noma. They analyse the problems and/or questions raised by humanitarian plastic surgery: sufficient training, choice of surgical techniques, postoperative follow-up, assessment of the results obtained, possible innovations.

[Falciform anemia and Plasmodium falciparum malaria: a threat to flap survival?]. / L'anémie falciforme et la malaria à Plasmodium falciparum: une menace pour la survie des lambeaux?

Plasmodium falciparum malaria, a parasitic disease, and sickle cell anemia, a hereditary disease, are two diseases affecting erythrocyte cycle, occurring with a high prevalence in tropical Africa. They may induce microthrombosis inducing vaso-occlusion, organ dysfunction and flap necrosis. During the acute phase of Plasmodium falciparum malaria, destruction of parasitized and healthy erythrocytes, release of parasite and erythrocyte material into the circulation, and secondary host reaction occur. Plasmodium falciparum infected erythrocytes also sequester in the microcirculation of vital organs and may interfere with microcirculatory flow in the flap during the postoperative period. The lower legs of homozygous sickle cell anemia patients are areas of marginal vascularity where minor abrasions become foci of inflammation. Inflammation results in decreased local oxygen tension, sickling of erythrocytes, increased blood viscosity and thrombosis with consequent ischemia, tissue breakdown and leg ulcer. Tissue transfer has become the procedure of choice for reconstruction of the lower third of the leg although flaps may become necrotic. The aim of this study is to analyse circumstances predisposing to surgical complications and to define preventive and therapeutic measures. A review of the literature will describe the current research and the new perspectives to treat sickle cell anemia, for example hydroxyurea and vasoactive substances (pentoxifylline, naftidrofuryl, buflomedil).

Craniofacial reconstruction of a giant cystlike temporal encephalocele.

A 4-year-old girl underwent craniofacial reconstruction for giant cystlike encephalocele deriving from the temporo-maxillary region and giving impression of the duplicated head. The case of temporal encephalocele in this report is especially unusual in the extent of encephalocele, the degree to which it had expanded the zygomatic arch, mandible, cranial vault, and the radiologically undetectable bony defect. The use of craniofacial principles in the resection and reconstruction of the temporal encephalocecle are described.

Nasal reconstruction in children: a review of 29 patients.

Acquired large nasal defects are much more common in adulthood than in childhood because of the frequency of skin tumors after a certain age. However, from their experience in treating a number of children with sequelae of noma and burns, the authors have collected a series of 17 total and 12 partial nasal reconstructions in children aged 1 to 15 years. After reviewing the various methods used for recreating the lining, the support, and the skin cover in the whole series, three cases are reported in detail. A 1-year-old patient received a tempororetroauricular flap after total amputation of the nose and was observed for 17 years. Another patient, who was burned as a baby, underwent reconstruction at age 10 with a deltopectoral flap and was observed for 7 years. The third patient underwent total nose reconstruction at age 12 with an Indian forehead flap. From their experience, the authors conclude that, for psychosocial reasons, nasal reconstruction should be started early, despite possible reoperation at a later age. The best results are certainly obtained at the end of growth or at least after the age of 12. Adjacent bone or soft tissue defects further enhance the difficult challenge of restoring a satisfactory aesthetic appearance in these children.

Gigantic neurofibromatosis of the orbit.

A case of gigantic orbital neurofibromatosis (2,500 gm) is presented. It is believed to be the largest such tumor reported since the famous elephantiasis neurofibromatosis pictured in von Bruns' Chirurgischer Atlas in 1857.

Covering by a flap induces apoptosis of granulation tissue myofibroblasts and vascular cells.

It has recently been shown that during the healing of an open wound, apoptosis mediates the decrease in cellularity during the transition between granulation tissue and scar. Because reduced contraction and a decrease in the number of fibroblastic cells have been described in wounds covered with a successful skin graft, we hypothesized that apoptosis could be responsible for these phenomena. Using in situ labeling of fragmented DNA, immunohistochemistry for alpha-smooth muscle actin, and electron microscopy, we have studied in rats the evolution of 10-day-old wound tissue covered with a total skin flap (containing epidermis, dermis, and the cutaneous muscle). In 10-day-old wound tissue, few apoptotic vascular cells and rare apoptotic myofibroblasts were present; the number of apoptotic cells increased slightly 72 hours later. In wounds covered with total skin flaps, the number of apoptotic vascular and myofibroblastic cells increased drastically 6 hours after flap application with a maximum at 24 and 48 hours, respectively. A decrease of apoptotic cell number was noted at 72 hours; at this time, the size of the granulation tissue was greatly reduced and showed extracellular matrix remodeling. Total flaps were more efficient in the induction of granulation tissue cell apoptosis compared with dermo-epidermal flaps. Moreover, the control application of full-thickness skin autografts, which were not viable 7 days later, did not induce apoptosis 24 hours after implantation. Our results indicate that covering granulation tissue with a skin flap results in a massive apoptotic process, possibly by means of a (some) locally released substance(s).

The sandwich epicranial flaps.

Sandwich epicranial flaps are prefabricated composite flaps that have multiple uses in reconstructing the middle and the lower facial region. Sandwich epicranial flaps always comprise the galea and a full-thickness skin graft; they may, in addition, include the temporal muscle and a fragment of parietal bone. According to their composition, four types of sandwich epicranial flaps are described. Experience with a use of 36 sandwich epicranial flaps (13 with vascularized bone) is reported. A few examples demonstrate the use of sandwich epicranial flaps in dramatic facial mutilation following noma in children. The sandwich epicranial flap can be performed at any age, does not require microsurgical anastomoses, and has a low complication rate. It is particularly useful for major reconstructive procedures in children.