ABSTRACT
The genus Sabethes (Diptera: Culicidae) comprises species of great epidemiological relevance, particularly involved in transmission cycles of the Yellow fever virus in South America. Given the unavailability of information related to aspects of evolutionary biology and molecular taxonomy of species of this genus of mosquitoes, we report here the first sequencing of the mitochondrial genomes of Sabethes bipartipes, Sabethes cyaneus, Sabethes tarsopus, and Sabethes quasicyaneus. The sequences obtained showed an average length of 14,920 bp, comprising 37 functional genes (13 PCGs, 22 tRNA, and 02 rRNA). The phylogenies reconstructed by Maximum likelihood and Bayesian inference methods, based on the concatenated sequences of all 13 PCGs, produced similar topologies and strongly supported the monophyletic relationship between the Sabethes subgenera, corroborating the known taxonomic classification based on aspects of the external morphology of the taxa assessed. The data and information produced from the Sabethes species evaluated here may be useful for future taxonomic and evolutionary studies of the genus, as well as the Culicidae family.
Subject(s)
Culicidae , Genome, Mitochondrial , Animals , Bayes Theorem , Culicidae/genetics , Phylogeny , South AmericaABSTRACT
The genus Aedes (Diptera: Culicidae) includes species of great epidemiological relevance, particularly involved in transmission cycles of leading arboviruses in the Brazilian Amazon region, such as the Zika virus (ZIKV), Dengue virus (DENV), Yellow fever virus (YFV), and Chikungunya virus (CHIKV). We report here the first putatively complete sequencing of the mitochondrial genomes of Brazilian populations of the species Aedes albopictus, Aedes scapularis and Aedes serratus. The sequences obtained showed an average length of 14,947 bp, comprising 37 functional subunits, typical in animal mitochondria (13 PCGs, 22 tRNA, and 2 rRNA). The phylogeny reconstructed by Maximum likelihood method, based on the concatenated sequences of all 13 PCGs produced at least two non-directly related groupings, composed of representatives of the subgenus Ochlerotatus and Stegomyia of the genus Aedes. The data and information produced here may be useful for future taxonomic and evolutionary studies of the genus Aedes, as well as the Culicidae family.
Subject(s)
Aedes , Culicidae , Genome, Mitochondrial , Zika Virus Infection , Zika Virus , Animals , Culicidae/genetics , Genome, Mitochondrial/genetics , Mosquito Vectors/genetics , Phylogeny , Zika Virus/geneticsABSTRACT
Amazon has been under intense human pressure, especially in the so-called "Arc of Deforestation" in the Eastern and Southern regions. Changes in biodiversity due to landscape disturbance in municipalities at the Arc of Deforestation are likely to impact mosquito species leading to the potential for emergence of arboviruses. Our aim was to describe the composition and structure of culicids in Sinop, a municipality located in the Southern Amazon at the State of Mato Grosso, as well as their presence in urban habitats, analyzing climate variables and potential risk of arbovirus transmission. Mosquitoes were collected in four habitats located in urban environments for 12 months. We recovered 5,210 adult mosquitoes from mosquito traps. Among the 33 species identified, 19 are associated with arbovirus transmission. Higher abundance, richness, diversity, and equitability of mosquito species were observed in urban forest parks and in new neighborhoods located in the outskirts of the city, nearby forest fragments and agricultural areas. As the environment consolidates as urban, both abundance and richness decrease. Highly urbanized neighborhoods had higher dominance of Culex quinquefasciatus, a mosquito associated with different arboviruses, including West Nile and Saint Louis encephalitis virus. The medically important species Cx. declarator, Cx. (Melanoconion) ssp., Aedeomyia squamipennis and Aedes scapularis were found in the four habitats. It is possible that these mosquitoes are adapting to different habitats and as a consequence, they are reaching urbanized areas. Mosquito abundance was higher in the days of high temperature and low precipitation in urban forest parks and in highly urbanized neighborhoods. These results suggest that Sinop region is a key area for surveillance of arbovirus vectors.
Subject(s)
Arboviruses/physiology , Culicidae/virology , Mosquito Vectors/virology , Animals , Biodiversity , Brazil , Cities , Climate , Culicidae/physiology , Ecosystem , Forests , Humans , Parks, Recreational , UrbanizationABSTRACT
The simultaneous circulation of Dengue virus (DENV), Chikungunya virus (CHIKV) and Zika virus (ZIKV) arboviruses have placed Brazil among the main worldwide endemic areas. Brazilian Northeast region concentrates the highest incidence of infections caused by CHIKV and ZIKV. In Maranhão, the second biggest northeastern state, there are cases of human infections caused by these three arboviruses and presence of Aedes aegypti and Aedes albopictus vectors. In this context, this study aimed to investigate the circulation of CHIKV, DENV and ZIKV in Ae. aegypti and Ae. albopictus mosquitoes collected in urban areas of Barra do Corda, Caxias, Codó, São Luís and São Mateus do Maranhão municipalities in the state of Maranhão through Quantitative Reverse Transcription Polymerase Chain Reaction (RT-qPCR) technique. 428 Ae. aegypti and 1 Ae. albopictus were collected, which formed 44 pools. Three of these showed positive results for CHIKV: AR832767 (five Ae. aegypti female collected in Caxias), AR832784 and AR832785 (both composed of 20 Ae. aegypti female collected in São Mateus do Maranhão). This study consolidates information about CHIKV circulation in state of Maranhão, as well as the role of Ae. aegypti in the transmission of CHIKV in urban area.
Subject(s)
Aedes/virology , Chikungunya Fever/transmission , Chikungunya Fever/virology , Chikungunya virus/isolation & purification , Animals , Brazil/epidemiology , Chikungunya virus/genetics , Dengue/transmission , Dengue/virology , Dengue Virus/genetics , Dengue Virus/isolation & purification , Endemic Diseases , Humans , Phylogeny , Real-Time Polymerase Chain Reaction , Zika Virus/genetics , Zika Virus/isolation & purification , Zika Virus Infection/transmission , Zika Virus Infection/virologyABSTRACT
Haemagogus janthinomys is a mosquito of high importance in public health due its involvement on natural wild cycles of two important arboviruses in the Brazilian Amazon region: Yellow Fever virus (Flaviviridae, Flavivirus) and Mayaro virus (Togaviridae, Alphavirus). Here, we have sequenced and described all the mitochondrial genes for the Hg. janthinomys species. The complete coding sequence is14 937 bp long and includes 37 functional genes, of which 13 codes for proteins, 22 for tRNA and 2 for ribosomal subunits. Region A + T (control region) is not presented here. The data should be helpful on further taxonomic and evolutionary studies of this important arbovirus vector.
Subject(s)
Culicidae/genetics , Genome, Mitochondrial , Animals , Base Composition , Brazil , Culicidae/classification , Culicidae/virology , DNA/chemistry , DNA/isolation & purification , DNA/metabolism , High-Throughput Nucleotide Sequencing , Insect Proteins/chemistry , Insect Proteins/genetics , Open Reading Frames/genetics , Phylogeny , RNA, Ribosomal/chemistry , RNA, Ribosomal/genetics , RNA, Transfer/chemistry , RNA, Transfer/genetics , Sequence Analysis, DNA , Yellow fever virus/physiologyABSTRACT
Epizootics and yellow fever epidemics in 2008 in Brazil, Paraguay and Argentina marked a significant progression of the disease further south in the continent affecting non-vaccinated human populations. The state of Mato Grosso do Sul is located between the tropical endemic region and the subtropical epidemic region and, despite being an area recommended for vaccination since the 1980s, human and animal cases of the disease are still registered. The purpose of this study was to present the results of entomological surveys of probable sites of infection (PSI) geographically describing these areas regarding human cases and disease suspicion reported in 2008. Thirteen locations in nine municipalities were investigated. A total of 305 females of the genus Haemagogus and Sabethes and five specimens of Aedes albopictus were obtained. The genus Haemagogus was more abundant but Sabethes presented twice as many species (3 x 6) and higher distribution within the collection points. The most abundant species was Hg. janthinomys, with 102 individuals in one location. The other species found were: Hg. leucocelaenus, Hg. spegazzinii, Sa. albiprivus, Sa. belisarioi, Sa. chloropterus, Sa. glaucodaemun, Sa. intermedius and Sa. soperi. It was not possible to identify predominant species in relation to environmental and geographical characteristics of the collection points. Of the nine human cases, eight corresponded to persons not resident in the State, seven of them tourists. MS features a large diversity of rural and wildlife tourist attractions where the yellow fever vectors described in this work are present. In this sense, the monitoring of vaccination coverage and flow of non-vaccinated people through the area are crucial to controlling the disease.
Subject(s)
Yellow Fever , Surveillance in Disasters , CulicidaeABSTRACT
INTRODUCTION: Knowledge concerning the fauna of Culicidae in the Brazilian Amazon States contributes to current understanding of the bionomics of the insects collected and makes it possible to observe changes in the fauna over time. METHODS: The Culicidae were captured with a BG-Sentinel® trap in extra-domiciliary area of two rural regions of Porto Velho in June and July of 2007 and 2008. RESULTS: A total of 10,695 Culicidae was collected, belonging to nine genera: Coquillettidia, Culex, Mansonia, Psorophora, Aedes, Aedeomyia, Anopheles, Uranotaenia and Wyeomyia. CONCLUSIONS: The presence of Mansonia (Mansonia) flaveola was recorded in the State of Rondônia for the first time.
Subject(s)
Culicidae/classification , Animals , Brazil , Female , Male , Rural HealthABSTRACT
INTRODUCTION: Knowledge concerning the fauna of Culicidae in the Brazilian Amazon States contributes to current understanding of the bionomics of the insects collected and makes it possible to observe changes in the fauna over time. METHODS: The Culicidae were captured with a BG-Sentinel® trap in extra-domiciliary area of two rural regions of Porto Velho in June and July of 2007 and 2008. RESULTS: A total of 10,695 Culicidae was collected, belonging to nine genera: Coquillettidia, Culex, Mansonia, Psorophora, Aedes, Aedeomyia, Anopheles, Uranotaenia and Wyeomyia. CONCLUSIONS: The presence of Mansonia (Mansonia) flaveola was recorded in the State of Rondônia for the first time.
INTRODUÇÃO: O conhecimento da fauna de culicídeos em estados da Amazônia Brasileira auxilia no conhecimento da bionomia dos insetos obtidos, além de possibilitar a observação de modificações da fauna ao decorrer do tempo. MÉTODOS: As capturas dos culicídeos foram realizadas em área extradomiciliar de duas zonas rurais de Porto Velho, durante junho e julho, nos anos de 2007 e 2008, com a armadilha BG-Sentinel®. RESULTADOs: Foram coletados 10.695 culicídeos, distribuídos em nove gêneros: Coquillettidia, Culex, Mansonia, Psorophora, Aedes, Aedeomyia, Anopheles, Uranotaenia e Wyeomyia. CONCLUSÕES: Foi registrada pela primeira vez no estado a presença de Mansonia (Mansonia) flaveola.
Subject(s)
Animals , Female , Male , Culicidae/classification , Brazil , Rural HealthABSTRACT
O presente estudo descreve os aspectos eco-epidemiológicos sobre arbovírus nos Municípios de Novo Progresso e Trairão, Estado do Pará, Brasil, na área de influência da BR 163. Anticorpos IH foram detectados para diferentes arbovírus, com reações monotípicas para os VMAY e VORO, dois importantes arbovírus associados a epidemias na Amazônia. Anticorpos IgM para o VORO e VMAY foram detectados em soros humanos, sugerindo infecção recente por esses arbovírus. Duas cepas do VDEN-3 foram isoladas de pacientes febris residentes em Novo Progresso e identificadas como genótipo III. Em termos gerais, os dados obtidos sugerem uma área propícia para a circulação e manutenção de arbovírus e uma população pouco imunizada. Portanto, é importante um monitoramento dinâmico das populações locais e de imigrantes e de animais silvestres quanto à presença de anticorpos e isolamentos de arbovírus, o que permitirá um efetivo controle das infecções por esses agentes virais em residentes da área da rodovia dentro do território paraense.
The current study describes the eco-epidemiological aspects of arbovirus diseases in the municipalities (counties) of Novo Progresso and Trairão, Para State, Brazil, in the area affected by highway BR-163. Hemagglutination inhibition (HI) antibodies to different arboviruses were detected, with monotypic reactions to MAYV and OROV, two important arboviruses associated with epidemics in the Amazon. IgM antibodies to OROV and MAYV were found in human sera, suggesting recent infections by these viruses. Two DENV-3 strains were isolated from febrile patients in Novo Progresso and identified as genotype III strains. In general, the data suggest that the area displays ideal conditions for maintenance and circulation of arboviruses, plus a population with low immunization levels. Dynamic surveillance of local immigrants and wild animals is thus important, focusing on antibody prevalence and isolation of arboviruses, thereby allowing effective control of infections by these viral agents in the resident population along highway BR-163 in Pará State.
Subject(s)
Adult , Animals , Female , Humans , Male , Antibodies, Viral/blood , Arbovirus Infections/epidemiology , Arbovirus Infections/veterinary , Arboviruses/immunology , Disease Outbreaks , Environmental Monitoring/statistics & numerical data , Arbovirus Infections/virology , Arthropod Vectors/virology , Brazil/epidemiology , Disease Vectors , Ecosystem , Immunoglobulin M/blood , Species Specificity , UrbanizationABSTRACT
The current study describes the eco-epidemiological aspects of arbovirus diseases in the municipalities (counties) of Novo Progresso and Trairão, Para State, Brazil, in the area affected by highway BR-163. Hemagglutination inhibition (HI) antibodies to different arboviruses were detected, with monotypic reactions to MAYV and OROV, two important arboviruses associated with epidemics in the Amazon. IgM antibodies to OROV and MAYV were found in human sera, suggesting recent infections by these viruses. Two DENV-3 strains were isolated from febrile patients in Novo Progresso and identified as genotype III strains. In general, the data suggest that the area displays ideal conditions for maintenance and circulation of arboviruses, plus a population with low immunization levels. Dynamic surveillance of local immigrants and wild animals is thus important, focusing on antibody prevalence and isolation of arboviruses, thereby allowing effective control of infections by these viral agents in the resident population along highway BR-163 in Pará State.
Subject(s)
Antibodies, Viral/blood , Arbovirus Infections/epidemiology , Arbovirus Infections/veterinary , Arboviruses/immunology , Disease Outbreaks , Environmental Monitoring/statistics & numerical data , Adult , Animals , Arbovirus Infections/virology , Arthropod Vectors/virology , Brazil/epidemiology , Disease Vectors , Ecosystem , Epidemiological Monitoring , Female , Humans , Immunoglobulin M/blood , Male , Species Specificity , UrbanizationABSTRACT
Oropouche fever has reemerged in Parauapebas and Porto de Moz municipalities, Pará State, Brazil. Serologic analysis (immunoglobulin M-ELISA) and virus isolation confirmed Oropouche virus (OROV) in both municipalities. Nucleotide sequencing of 2 OROV isolates from each location indicated genotypes I (Parauapebas) and II (Porto de Moz) in Brazil.
Subject(s)
Bunyaviridae Infections/epidemiology , Disease Outbreaks , Simbu virus/genetics , Adolescent , Brazil/epidemiology , Bunyaviridae Infections/genetics , Child , Child, Preschool , Communicable Diseases, Emerging , Enzyme-Linked Immunosorbent Assay , Female , Genotype , Humans , Infant , Male , Molecular Sequence Data , Phylogeny , Simbu virus/classification , Simbu virus/pathogenicityABSTRACT
For the first time, the occurrence of Aedes (Stegomyia) albopictus in an urban area of the city of Fortaleza, Northeastern, Brazil, is reported. From January to July 2005, ovitraps were used to collect eggs from Aedes spp., which were kept under laboratory conditions to develop into the adult phase. The resultant mosquitoes were identified and subjected to dengue virus isolation tests. Thirteen specimens of Aedes albopictus, all females, were identified. No dengue virus was isolated in any of the mosquito pools. Even though Aedes albopictus has not been incriminated in Brazilian dengue outbreaks, the possibility of dengue virus transmission by these mosquitoes cannot be dismissed.
Subject(s)
Aedes/classification , Dengue Virus , Insect Vectors , Aedes/physiology , Animals , Brazil , Dengue/transmission , Dengue Virus/isolation & purification , Female , Humans , Insect Vectors/physiology , Male , Species Specificity , Urban PopulationABSTRACT
Pela primeira vez é registrada a ocorrência de Aedes (Stegomyia) albopictus em área urbana da cidade de Fortaleza, no Estado do Ceará, Brasil. De janeiro a julho de 2005 foram utilizadas ovitrampas para a coleta de ovos de Aedes spp., os quais foram mantidos em laboratório para desenvolvimento até a fase adulta. Os mosquitos resultantes foram identificados e submetidos a testes para o isolamento dos vírus da dengue. Foram identificados 13 espécimes de Aedes albopictus, todos fêmeas. Não foi isolado vírus da dengue em nenhum dos pools de mosquitos. Apesar de o Aedes albopictus não ter sido incriminado por surtos de dengue no Brasil, não se pode descartar a possibilidade da transmissão dos vírus da dengue por tais mosquitos.
For the first time, the occurrence of Aedes (Stegomyia) albopictus in an urban area of the city of Fortaleza, Northeastern, Brazil, is reported. From January to July 2005, ovitraps were used to collect eggs from Aedes spp., which were kept under laboratory conditions to develop into the adult phase. The resultant mosquitoes were identified and subjected to dengue virus isolation tests. Thirteen specimens of Aedes albopictus, all females, were identified. No dengue virus was isolated in any of the mosquito pools. Even though Aedes albopictus has not been incriminated in Brazilian dengue outbreaks, the possibility of dengue virus transmission by these mosquitoes cannot be dismissed.
Subject(s)
Dengue Virus , AedesSubject(s)
Culicidae/physiology , Radio , Sound , Animals , Female , Insect Bites and Stings , Models, StatisticalABSTRACT
It is first reported the detection of Aedes (Stg) albopictus mosquitoes in state of Par , Brazil, in the urban area of Medicil ndia, a municipality far 90 km from Altamira, where 42 adult mosquitoes were baited using human attraction. All mosquitoes were pooled and inoculated into C6/36 and suckling mice in attempts for virus isolation. No virus was isolated. The occurrence of Aedes albopictus in urban areas of the Amazon region is of concern since dengue and yellow fever viruses are endemic in the Amazon and thus there is a potential risk for this mosquito species to become infected with both viruses.
Subject(s)
Aedes/virology , Dengue Virus/isolation & purification , Dengue/transmission , Insect Vectors/virology , Animals , Animals, Newborn , Brazil , Dengue/virology , Female , Humans , Male , Mice , Species Specificity , Yellow Fever/transmission , Yellow fever virus/isolation & purificationABSTRACT
Pela primeira vez é registrada a presença do Aedes (Stg) albopictus no Estado do Pará, Brasil, em área urbana no município de Medicilândia distante cerca de 90 km de Altamira, onde foram capturados por meio de isca humana 42 exemplares de mosquitos adultos. Estes foram inoculados em C6/36 e em camundongos recém-nascidos na tentativa de isolamento viral, näo tendo sido isolado nenhum vírus. A presença de Aedes albopictus em áreas da Amazônia onde circulam os vírus de dengue e de febre amarela é preocupante e representa um risco potencial desta espécie de mosquito se tornar infectada com tais vírus