Human papillomavirus in women infected with human immunodeficiency virus: association with viral load and lymphocyte count.

Women living with human immunodeficiency virus are at an increased risk of developing cancers related to human papillomavirus (HPV). Thus, it is important to combine clinical assessments, serological screening, and HPV data for planning prevention policies. This study aimed to identify HPV and its specific types in the cervical, anal, and oral mucosa of HIV-seropositive women, associating it with viral load and lymphocyte count. Sociodemographic characteristics, health data (CD4+ and CD8+ T cell counts and viral load), and biological samples (cervical, anal, and oral) were collected from 86 HIV-positive women undergoing antiretroviral therapy. Data were classified according to the presence or absence of HPV-DNA, HPV-DNA presence at one or more anatomic sites, and level of oncogenic risk, considering low- and high-risk oncogenic HPV-DNA groups. The presence of HPV in the cervicovaginal site was 65.9%, 63.8% in anal canal, and 4.2% in oral mucosa. A viral load ≥75 HIV copies/mL was associated with the presence of HPV-DNA. There was an association between viral load and the low-risk HPV or high-risk HPV groups. We found a high prevalence of HPV infection in HIV-seropositive women, particularly in the cervical and anal mucosa, with viral load ≥75 HIV copies/mL being associated with HPV-DNA presence.

Human papillomavirus in women infected with human immunodeficiency virus: association with viral load and lymphocyte count

ABSTRACT Women living with human immunodeficiency virus are at an increased risk of developing cancers related to human papillomavirus (HPV). Thus, it is important to combine clinical assessments, serological screening, and HPV data for planning prevention policies. This study aimed to identify HPV and its specific types in the cervical, anal, and oral mucosa of HIV-seropositive women, associating it with viral load and lymphocyte count. Sociodemographic characteristics, health data (CD4+ and CD8+ T cell counts and viral load), and biological samples (cervical, anal, and oral) were collected from 86 HIV-positive women undergoing antiretroviral therapy. Data were classified according to the presence or absence of HPV-DNA, HPV-DNA presence at one or more anatomic sites, and level of oncogenic risk, considering low- and high-risk oncogenic HPV-DNA groups. The presence of HPV in the cervicovaginal site was 65.9%, 63.8% in anal canal, and 4.2% in oral mucosa. A viral load ≥75 HIV copies/mL was associated with the presence of HPV-DNA. There was an association between viral load and the low-risk HPV or high-risk HPV groups. We found a high prevalence of HPV infection in HIV-seropositive women, particularly in the cervical and anal mucosa, with viral load ≥75 HIV copies/mL being associated with HPV-DNA presence.

Perfil Demográfico e Clínico de Casos de Neoplasias Hematológicas em Crianças e Adolescentes / Demographic and Clinical Profile of Cases of Hematological Neoplasms in Children and Adolescents / Perfil Demográfico y Clínico de Casos de Neoplasias Hematológicas en Niños y Adolescentes

Introdução: As neoplasias hematológicas são importantes causas de morte na infância e adolescência. Objetivo: Caracterizar o perfil demográfico e clínico de casos notificados de neoplasias hematológicas em crianças e adolescentes em um hospital de referência do município de São Luís, Maranhão, Brasil. Método: Estudo observacional retrospectivo com dados coletados de prontuários médicos de casos de neoplasias hematológicas em pacientes com idade até 19 anos registrados em um hospital público de referência para tratamento oncológico, no período de 2005 a 2015. Foram coletados dados de caracterização geral dos pacientes e dados do seguimento do tratamento. Os testes qui-quadrado e exato de Fisher foram utilizados na análise estatística. O nível de significância adotado foi de 5% (p<0,05). Resultados: Os casos de óbito foram mais elevados nos pacientes diagnosticados com linfoma não Hodgkin. A maioria dos pacientes com leucemia linfoide era do sexo masculino com idade até 5 anos, e a frequência de óbito nos pacientes com leucemia linfoide foi mais baixa nos casos confirmados de leucemia linfoblástica de células precursoras tipo B em comparação a outras categorias. Conclusão: Os achados sugerem que a maioria dos pacientes com até 19 anos diagnosticados com leucemia linfoide era do sexo masculino e com idade até 5 anos

Introduction: Hematological cancersare important causes of death in childhood and adolescence. Objective: To design the demographic and clinical profile of cases of hematological cancer reported for children and adolescents in a referral hospital in the city of São Luís, Maranhão, Brazil. Method: A retrospective observational study with secondary data collected from medical records of bone marrow tumor cases in patients aged up to 19 years registered in a public referral hospital for cancer treatment from 2005 to 2015. Patient's profile was collected in addition to follow-up data. The chi-square and Fisher's exact test were used in the statistical analysis. The significance level was 5% (p<0.05). Results: Cases of death were higher in patients diagnosed with non-Hodgkin's lymphoma. Most patients with lymphoid leukemia were males aged up to 5 years, and the frequency of death in patients with lymphoid leukemia was lower in confirmed cases of type B lymphoblastic precursor cell leukemia when compared to the other categories. Conclusion: The findings suggest that most patients aged up to 19 years diagnosed with lymphoid leukemia were males and aged up to 5 years old

Introducción: Las neoplasias hematológicas son causas importantes de muerte en la niñez y la adolescencia. Objetivo: Caracterizar el perfil demográfico y clínico de los casos notificados de neoplasias hematológicas en niños y adolescentes en un hospital de referencia de la ciudad de São Luís, Maranhão, Brasil. Método: estudio observacional retrospectivo con datos secundarios recolectados de registros médicos de casos de tumores de médula ósea en pacientes de hasta 19 años registrados en un hospital público de referencia para tratamiento de cáncer de 2005 a 2015. Se recolectaron datos de caracterización. Datos generales de pacientes y seguimiento -up datos. En el análisis estadístico se utilizaron las pruebas de chi-cuadrado y exacta de Fisher. El nivel de significancia adoptado fue del 5% (p<0,05). Resultados:Los casos de muerte fueron mayores en pacientes con diagnóstico de linfoma no Hodgkin. La mayoría de los pacientes con leucemia linfoide eran varones de hasta 5 años, y la frecuencia de muerte en pacientes con leucemia linfoide fue menor en los casos confirmados de leucemia de células precursoras linfoblásticas tipo B en comparación con las otras categorías. Conclusión:Los hallazgos sugieren que la mayoría de los pacientes de hasta 19 años diagnosticados con leucemia linfoide eran hombres y tenían hasta 5 años

Recurrent respiratory papillomatosis: clinical characteristics and viral genotyping in a Brazilian population.

This study presents 25 cases of recurrent respiratory papillomatosis (RRP) that occurred in Sao Luis, Maranhao State, Northeast region, Brazil, between January 2007 and December 2018. Sociodemographic and clinical profile of patients as well as human papillomavirus (HPV) infection status were evaluated. Clinical and histopathological data were collected from the patients' medical records. For the HPV infection analysis, DNA was extracted and subjected to amplification by a nested polymerase chain reaction. Viral genotyping was performed by automated sequencing. The median age of patients was 12.40 ± 12.6. years, and the juvenile form of the disease (68%) was the predominant form of disease. Female participants were predominant (60%), and they were from cities located in the interior of the State (60%). The most common clinical manifestation was dysphonia; recurrence was observed in most cases (56%), and tracheostomy was necessary in seven patients (26.9%). When comparing the RRP forms, patients in the juvenile-RRP group had higher recurrence rates and need of tracheostomy than those in the adult-RRP group. The viral genotyping analysis revealed that 47.8% of patients had low-risk HPVs, whereas 13.1% had high-risk HPVs, and in 39.1% of patients the viral genotype was not obtained. HPV-6 was the most prevalent type and Juvenile-RRP was more prevalent in our population. HPV was present at a high rate, and HPV-6 was the predominant genotype. This study serves as the basis for further studies to be conducted in the Brazilian population. Our findings aid the better understanding of RRP, possibly suggesting some prognostic factors associated with the disease aggressiveness.