ABSTRACT
This article outlines the trajectory of the Brazilian Association of Nursing (ABEn) in Alagoas and its influence in the organization of the nursing profession in this federal state. It points out that ABEn-AL was the first institution to organize nursing in Alagoas, since it was founded prior to any nursing graduation course or even any nursing syndicate. Therefore, ABEn is considered a decisive landmark in the political and professional organization of nursing in the state of Alagoas.
Subject(s)
Societies, Nursing/history , Brazil , History, 20th Century , Societies, Nursing/organization & administrationABSTRACT
Treatment of tachyzoites of Toxoplasma gondii with the calcium ionophore A23187 induced dramatic ultrastructural changes that were observed by light and electron microscopy. Light microscopy showed a higher percentage (22%) of tachyzoites with the conoid extruded when compared to control parasites. Electron microscopy confirmed the conoid extrusion by both transmission and scanning electron microscopy. Freeze-fracture replicas showed that the plasma membrane adjacent to cytoplasmic dense granules appeared devoid of intramembranous particles. Membrane-limited vesicles and filopodium-like structures at the cell surface were observed in treated cells. 3-D reconstruction from serial sections confirmed the data and showed a heterogeneity in dense granule shape not reported in control cells.
Subject(s)
Calcimycin/pharmacology , Toxoplasma/ultrastructure , Animals , Cell Membrane/ultrastructure , Freeze Fracturing , Image Processing, Computer-Assisted , Intracellular Membranes/ultrastructure , Ionophores/pharmacology , Microscopy, Electron , Toxoplasma/drug effects , Toxoplasma/physiologyABSTRACT
Tachyzoites of Toxoplasma gondii are able to infect several cell types tested (wild-type chinese hamster ovary (CHO) cells and glycosylation mutants, Vero and LLCMK2 cells). However, the extent of infection varied. Mutant cells which present few or no surface-exposed sialic acid residues were infected to a lower extent. Similar results were obtained if sialic acid residues were removed by previous neuraminidase treatment. Addition of sialic acid residues to surface-exposed glycoconjugates using fetuin as a sialic acid donor and the trans-sialidase of Trypanosoma cruzi rendered the cells more easily infected by Toxoplasma gondii. These observations indicate that surface-exposed carbohydrate residues of the host cell are involved on the process of Toxoplasma gondii-host cell recognition.
