Ultrasound Therapy With High-Pressure Pulses Is Effective to Reduce the Effects of Collagenase-Induced Tendinopathy in Rat's Achilles Tendon.

OBJECTIVE: We propose an ultrasonic treatment for collagenase-induced tendinopathy in rat's Achilles tendon using pulses with a low number of cycles, high acoustic pressure and very low duty cycle. METHODS: Twenty rats were used to perform the experiment. Four experimental groups of calcaneal tendons were studied: control (n = 6), sham (n = 4), collagenase-induced tendinopathy (n = 8) and ultrasound-treated collagenase-induced tendinopathy (n = 8). Surgical intervention was performed to expose the tendons prior to collagenase injection. A 1 MHz ultrasonic tansducer with a focusing lens was used. Ultrasonic treatments were used with an average total treatment time of 2.5 min, 20-cycle pulses, pressure amplitude p = 7 MPa, and 0.02% duty cycle. Histopathology of the samples was performed to evaluate nuclear density, acute inflammation, and signs of neovascularization. Collagen (types I and III), elastic fibers, and glycosaminoglycans were also analyzed. RESULTS: No tendon involvement was found by the surgical process. Ultrasonic treatment is safe, as it does not affect healthy tendons. When collagenase infiltrated animals were treated with US, a clear predominance of type I collagen fibers and a similar collagen ratio profile to that observed in the control and sham groups was observed, with a higher density of elastic fibers compared to the control and sham groups and a significant increase in the density of glycosaminoglycans. CONCLUSION: The ultrasound treatment proposed reduces the effects of the artificial collagenase lesion to reach the basal level after 45 d.

Device for Dual Ultrasound and Dry Needling Trigger Points Treatment.

Ultrasound is a well-known tool to produce thermal and non-thermal effects on cells and tissues. These effects require an appropriate application of ultrasound in terms of localization and acoustic energy delivered. This article describes a new device that combines ultrasound and dry needling treatments. The non-thermal effects of ultrasound should locally amplify the needle's effects. The ultrasound transducer can mechanically rotate in 3D space to align itself in the direction of the needle. The transducer electronically focuses the acoustic pressure automatically on the needle tip and its surroundings. A computer, using graphical interface software, controls the angulation of the array and the focus position.

Effects of Non-thermal Ultrasound on a Fibroblast Monolayer Culture: Influence of Pulse Number and Pulse Repetition Frequency.

Despite the use of therapeutic ultrasound in the treatment of soft tissue pathologies, there remains some controversy regarding its efficacy. In order to develop new treatment protocols, it is a common practice to carry out in vitro studies in cell cultures before conducting animal tests. The lack of reproducibility of the experimental results observed in the literature concerning in vitro experiments motivated us to establish a methodology for characterizing the acoustic field in culture plate wells. In this work, such acoustic fields are fully characterized in a real experimental configuration, with the transducer being placed in contact with the surface of a standard 12-well culture plate. To study the non-thermal effects of ultrasound on fibroblasts, two different treatment protocols are proposed: long pulse (200 cycles) signals, which give rise to a standing wave in the well with the presence of cavitation (ISPTP max = 19.25 W/cm2), and a short pulse (five cycles) of high acoustic pressure, which produces a number of echoes in the cavity (ISPTP = 33.1 W/cm2, with Pmax = 1.01 MPa). The influence of the acoustic intensity, the number of pulses, and the pulse repetition frequency was studied. We further analyzed the correlation of these acoustic parameters with cell viability, population, occupied surface, and cell morphology. Lytic effects when cavitation was present, as well as mechanotransduction reactions, were observed.

Study of the Relation between the Resonance Behavior of Thickness Shear Mode (TSM) Sensors and the Mechanical Characteristics of Biofilms.

This work analyzes some key aspects of the behavior of sensors based on piezoelectric Thickness Shear Mode (TSM) resonators to study and monitor microbial biofilms. The operation of these sensors is based on the analysis of their resonance properties (both resonance frequency and dissipation factor) that vary in contact with the analyzed sample. This work shows that different variations during the microorganism growth can be detected by the sensors and highlights which of these changes are indicative of biofilm formation. TSM sensors have been used to monitor in real time the development of Staphylococcus epidermidis and Escherichia coli biofilms, formed on the gold electrode of the quartz crystal resonators, without any coating. Strains with different ability to produce biofilm have been tested. It was shown that, once a first homogeneous adhesion of bacteria was produced on the substrate, the biofilm can be considered as a semi-infinite layer and the quartz sensor reflects only the viscoelastic properties of the region immediately adjacent to the resonator, not being sensitive to upper layers of the biofilm. The experiments allow the microrheological evaluation of the complex shear modulus (G* = G' + jG″) of the biofilm at 5 MHz and at 15 MHz, showing that the characteristic parameter that indicates the adhesion of a biofilm for the case of S. epidermidis and E. coli, is an increase in the resonance frequency shift of the quartz crystal sensor, which is connected with an increase of the real shear modulus, related to the elasticity or stiffness of the layer. In addition both the real and the imaginary shear modulus are frequency dependent at these high frequencies in biofilms.

The Use of Phononic Crystals to Design Piezoelectric Power Transducers.

It was recently proposed that the lateral resonances around the working resonance band of ultrasonic piezoelectric sandwich transducers can be stopped by a periodic array of circular holes drilled along the main propagation direction (a phononic crystal). In this work, the performance of different transducer designs made with this procedure is tested using laser vibrometry, electric impedance tests and finite element models (FEM). It is shown that in terms of mechanical vibration amplitude and acoustic efficiency, the best design for physiotherapy applications is when both, the piezoceramic and an aluminum capsule are phononic structures. The procedure described here can be applied to the design of power ultrasonic devices, physiotherapy transducers and other external medical power ultrasound applications where piston-like vibration in a narrow band is required.

Noncontact monitoring of incision depth in laser surgery with air-coupled ultrasound transducers.

Lack of haptic feedback during laser surgery makes it difficult to control the incision depth, leading to high risk of undesired tissue damage. Here, we present a new feedback sensing method that accomplishes noncontact real-time monitoring of laser ablation procedures by detecting shock waves emanating from the ablation spot with air-coupled transducers. Experiments in soft and hard tissue samples attained high reproducibility in real-time depth estimation of the laser-induced cuts. The advantages derived from the noncontact nature of the suggested monitoring approach are expected to advance the general applicability of laser-based surgeries.

Colloidal Stability and Magnetic Field-Induced Ordering of Magnetorheological Fluids Studied with a Quartz Crystal Microbalance.

This work proposes the use of quartz crystal microbalances (QCMs) as a method to analyze and characterize magnetorheological (MR) fluids. QCM devices are sensitive to changes in mass, surface interactions, and viscoelastic properties of the medium contacting its surface. These features make the QCM suitable to study MR fluids and their response to variable environmental conditions. MR fluids change their structure and viscoelastic properties under the action of an external magnetic field, this change being determined by the particle volume fraction, the magnetic field strength, and the presence of thixotropic agents among other factors. In this work, the measurement of the resonance parameters (resonance frequency and dissipation factor) of a QCM are used to analyze the behavior of MR fluids in static conditions (that is, in the absence of external mechanical stresses). The influence of sedimentation under gravity and the application of magnetic fields on the shifts of resonance frequency and dissipation factor were measured and discussed in the frame of the coupled resonance produced by particles touching the QCM surface. Furthermore, the MR-fluid/QCM system has a great potential for the study of high-frequency contact mechanics because the translational and rotational stiffness of the link between the surface and the particles can be tuned by the magnetic field.

Study of the effect of particle volume fraction on the microstructure of magnetorheological fluids using ultrasound: Transition between the strong-link to the weak-link regimes.

The effect of particle volume fraction on the microstructure of magnetorheological (MR) fluids has been studied using ultrasonic techniques. When no magnetic field is applied, they behave as slurry. However, when magnetic field is applied, important features regarding the change of the microstructure have been found with the help of ultrasonic waves propagating in the direction of the magnetic field. As the volume fraction increases, a rearrangement of particles which decrease the compressibility of the system is detected; nevertheless, the material behaves as a non-consolidated material. Three different particle volume fraction regions are found identifying a critical particle volume fraction predicted in the literature. Ultrasounds are confirmed as an interesting tool to study MR fluids in static conditions.

Air-coupled MUMPs capacitive micromachined ultrasonic transducers with resonant cavities.

This work reports performance improvements of air-coupled capacitive micromachined ultrasonic transducers (CMUTs) using resonant cavities. In order to perform this work, we have designed and manufactured a CMUT employing multi-user microelectromechanical systems (MEMS) processes (MUMPs). The transducer was designed using Helmholtz resonator principles. This was characterised by the dimensions of the cavity and several acoustic ports, which had the form of holes in the CMUT plate. The MUMPs process has the advantage of being low cost which allows the manufacture of economic prototypes. In this paper we show the effects of the resonant cavities and acoustic ports in CMUTs using laser Doppler vibrometry and acoustical measurements. We also use Finite Element (FE) simulations in order to support experimental measurements. The results show that it is possible to enhance the output pressure and bandwidth in air by tuning the resonance frequency of the plate (f(p)) with that of the Helmholtz resonator (f(H)). The experimental measurements show the plate resonance along with an additional resonance in the output pressure spectrum. This appears due to the effect of the new resonant cavities in the transducer. FE simulations show an increase of 11 dB in the output pressure with respect to that of a theoretical vacuum-sealed cavity MUMPs CMUT by properly tuning the transducer. The bandwidth has been also analyzed by calculating the mechanical Q factor of the tuned CMUT. This has been estimated as 4.5 compared with 7.75 for the vacuum-sealed cavity MUMPs CMUT.

Damage characterization using guided-wave linear arrays and image compounding techniques.

In this work, a high-resolution imaging method for the inspection of isotropic plate-like structures using linear arrays and Lamb waves is proposed. The evaluation of these components is limited by the low dynamic range resulting from main lobe and side lobe field patterns, and from the narrowband nature of the Lamb waves. Based on a full matrix array, synthetic aperture technique using all emitter-receiver combinations, different images from the same object are obtained by using different apodization coefficients, which are related to a trade-off between main lobe width and relative side lobe level. Several image compounding strategies have been tested and a new algorithm, based on apodization and polarity diversities between signals, is proposed. However, some effects, such as the dead zone close to the array and reverberations caused by interactions of the wavefront and defects, still limit the quality of the images. The use of spatial diversity, obtained by an additional array, introduces complementary information about the defects and improves the results of the proposed algorithm, producing high-resolution, high-contrast images. Experimental results are shown for a 1-mm-thick isotropic aluminum plate with artificial defects using linear arrays formed by 30 piezoelectric elements, with the low dispersion symmetric mode S0 at the frequency of 330 kHz.

Double frequency piezoelectric transducer design for harmonic imaging purposes in NDT.

Harmonic imaging (HI) has emerged as a very promising tool for medical imaging, although there has been little published work using this technique in ultrasonic nondestructive testing (NDT). The core of the technique, which uses nonlinear propagation effects arising in the medium due to the microstructure or the existence of defects, is the ability to design transducers capable of emitting at one frequency and receiving at twice this frequency. The transducers that have been used so far are usually double crystal configurations with coaxial geometry, and commonly using a disc surrounded by a ring. Such a geometry permits the design of broadband transducers if each transducer element is adapted to the medium with its corresponding matching layers. Nevertheless, the different geometry of the emission and reception apertures creates difficulties when resolving the images. In this work, a new transducer design with different emission and reception apertures is resented. It makes use of the traditional construction procedures used to make piezocomposite transducers and the well-known theory of the mode coupling in piezoelectric resonators when the lateral dimensions are comparable with the thickness of the piezoceramic. In this work the design, construction, and characterization of a prototype to be used in NDT of metallic materials is presented. The acoustic field is calculated using water as a propagation medium, and these theoretical predictions then are compared with the experimental measurements. The predicted acoustic performances for the case of propagation in stainless steel are shown.

A computational method to calculate the longitudinal wave evolution caused by interfaces between isotropic media.

This paper presents a computational method to calculate the reflected and transmitted ultrasonic fields at interfaces of complex geometry. The method is performed in two steps. As first step, the velocity potential impulse response from an arbitrary aperture is determined at the interface using the Rayleigh integral and considering the reflection and transmission coefficients. In a second step, the simulated fields are calculated by applying the Rayleigh-Sommerfeld integral to the whole, extended interface. In order to validate the method, some experimental cases as, for instance, plane and cylindrical concave surfaces between two media (water-acrylic) were tested. The experimental ultrasonic fields are in good agreement with those provided by the model. Furthermore, in the work, the compromise between the accuracy of the method and the computation time is studied.

3D computational method to study the focal laws of transducer arrays for NDE applications.

Based on the impulse response and the discrete representation methods, a 3D computational method has been developed to calculate the optimal focal laws to focus the ultrasonic beams through interfaces of complex geometry, and the respective transmitted ultrasonic field generated by NDE transducer arrays. 1- and 2D array transducers are considered. Two different focusing techniques are used to obtain the time delays: the first travel time on each center of the array element, and the cross correlation between the simulated signals from neighboring array elements. Applying the time delays to the array, the transmitted field can be simulated using the same computational method. Several simulations were performed to present the ability of the computational method to focus through, for instance, curved and plane surfaces between two media (acrylic-steel). A comparison between the two focusing techniques is presented.

Contribución al diagnóstico precoz de bacteriemia: detección del crecimiento microbiano en medios de cultivo líquidos por ultrasonidos / Contribution to the early diagnosis of bacteremia: microbial growth detection in liquids by ultrasound

La infección nosocomial es un problema importante que afecta a un gran número de pacientes hospitalizados; requiriendo de los laboratorios de cada centro un esfuerzo encaminado a detectar precozmente los microorganismos responsables de bacteriemias y fungemias. Los ultrasonidos son ampliamente utilizados con fines diagnósticos en medicina y es conocida la posibilidad de medir con ellos parámetros físicos y químicos de medios líquidos, usando las variables de la velocidad de propagación y atenuación. Nuestro objetivo fue desarrollar un sistema analítico automatizado, diseñado para detectar, de forma eficaz y no invasora, el crecimiento precoz de microorganismos en medios de cultivo líquidos sembrados con muestras clínicas diversas. Para ello se procedió a la medición de la velocidad de propagación de un pulso ultrasónico a través de frascos de hemocultivo, con muestra de sangre o sin ella, en los que se habían inoculado concentraciones conocidas de microorganismos frecuentemente implicados en cuadros sépticos. El gas carbónico generado por las bacterias en crecimiento modifica la velocidad de propagación del sonido. La monitorización continua del sistema nos permite obtener una representación gráfica del crecimiento microbiano y aplicar una serie de algoritmos diagnósticos relacionados con la pendiente de la velocidad a fin de conocer con la mayor rapidez los cambios físicos que se producen, en el medio líquido, cuando los microorganismos están presentes. El tiempo de detección por nuestro sistema es reducido y se ha mostrado similar o discretamente mejor que otros comercializados. El método es sencillo, económico e innovador y abre la posibilidad de nuevas aplicaciones con fines diagnósticos en microbiología (AU)