Targeted reproductive management for lactating Holstein cows: Reducing the reliance on exogenous reproductive hormones.

Adoption of automated monitoring devices (AMD) affords the opportunity to tailor reproductive management according to the cow's needs. We hypothesized that a targeted reproductive management (TRM) would reduce the use of reproductive hormones while increasing the percentage of cows pregnant 305 d in milk (DIM). Holstein cows from 2 herds (n = 1,930) were fitted with an AMD at 251.0 ± 0.4 d of gestation. Early-postpartum estrus characteristics (EPEC; intense estrus = heat index ≥70; 0 = minimum, 100 = maximum) of multiparous cows were evaluated at 40 (herd 1) or 41 (herd 2) DIM and EPEC of primiparous cows were evaluated at 54 (herd 1) or 55 (herd 2) DIM. Control cows received the first artificial insemination at fixed time (TAI; primiparous, herd 1 = 82 and herd 2 = 83 DIM; multiparous, herd 1 = 68 and herd 2 = 69 DIM) following the Double-Ovsynch (DOV) protocol. Cows enrolled in the TRM treatment were managed as follows: (1) cows with at least one intense estrus were inseminated upon AMD detected estrus for 42 d and, if not inseminated, were enrolled in the DOV protocol; and (2) cows without an intense estrus were enrolled in the DOV protocol at the same time as cows in the control treatment. Control cows were re-inseminated based on visual or patch aided detection of estrus, whereas TRM cows were re-inseminated as described for control cows with the aid of the AMD. Cows received a GnRH injection 27 ± 3 d after insemination and, if diagnosed as nonpregnant, completed the 5-d Cosynch protocol and received TAI 35 ± 3 d after insemination. Among cows in the TRM treatment, 55.8 and 42.9% of primiparous and multiparous cows, respectively, received the first insemination in spontaneous estrus. The interaction between treatment and parity affected pregnancy 67 d after the first AI (primiparous: control = 37.6%, TRM = 27.4%; multiparous: control = 41.0%, TRM = 44.7%). The TRM treatment increased re-insemination in estrus (control = 48.3%, TRM = 70.5%). Pregnancy 67 d after re-inseminations tended to be affected by the interaction between treatment and EPEC (no intense estrus: control = 25.3%, TRM = 32.0%; intense estrus: control = 32.9%, TRM = 32.2%). The interaction between treatment and EPEC affected pregnancy by 305 DIM (no intense estrus: control = 80.8%, TRM = 88.2%; intense estrus: control = 87.1%, TRM = 86.1%). Treatment did not affect the number of reproductive hormone treatments among cows that had not had an intense estrus (control = 10.5 ± 0.3, TRM = 9.1 ± 0.2 treatments/cow), but cows in the TRM treatment that had an intense estrus received fewer reproductive hormone treatments than cows in the control treatment (2.0 ± 0.1 vs. 9.6 ± 0.2 treatments/cow). Selecting multiparous cows for first AI in estrus based on EPEC reduced the use of reproductive hormones without impairing the likelihood of pregnancy to first AI. The use of AMD for re-insemination expedited the establishment of pregnancy among cows that did not display an intense estrus early postpartum.

A randomized trial on the effects of heat stress abatement on environmental conditions and growth, feed efficiency, and concentration of metabolites of pre-weaned female Holstein calves.

Heat abatement strategies for pre-weaned calves are seldom adopted. Our objectives were to determine the effects of adding fans to barns on environmental conditions and growth, feed efficiency, concentration of metabolites and health of pre-weaned female Holstein calves. Calves born from July 15th to 30th of 2019 were eligible for enrollment. At birth (d 0), calves were assigned randomly to: SH (n = 125) - hutch under a barn with no cooling, SHF (n = 101) - hutch under a barn cooled through fans. Body weight (BW) and wither-height were measured at birth and d 68. Calves were evaluated thrice weekly (0700-1000 h) using the Calf Health Scoring Chart (UW-Madison). A sub-sample of hutches (SH = 26, SHF = 25) was evaluated for air velocity and temperature at 1000 and 1600 h thrice weekly and calves housed in these hutches were evaluated for rectal temperature (RT) at 1600 h and respiratory rate (RR) at 1000 and 1600 h. Calves were fed a liquid diet twice a day (d 2-18 = 5.56 L/d; d 19-49 = 7.58 L/d; d 50-56 = 3.84 L/d; d 57-63 = 1.64 L/d) and starter ad libitum starting on d 14. A sub-sample of calves (SH = 56, SHF = 44) had intakes of liquid feed and starter measured daily, BW and wither-height measured weekly from birth to d 68, and blood sampled on d 1, 14, 28, 42, 49, 52, 56, 58, 63 and 65 for the measurement of fatty acids, ß-hydroxybutyrate, and glucose concentrations. The SHF treatment increased air velocity by 0.8 m/sec and reduced air temperature by 0.3 ºC. The SHF treatment reduced RT (38.70 ± 0.03 vs. 38.78 ± 0.02 °C) and the percentage of calves with hyperthermia (RT ≥ 39.2 °C; 20.6 ± 1.9 vs. 30.2 ± 2.0%) at 1000 h. Treatment did not affect feed efficiency (SH = 0.53 ± 0.01, SHF = 0.53 ± 0.01 g of BW gained/g of dry matter intake), nor did it affect BW (SH = 81.6 ± 0.7, SHF = 82.9 ± 0.8 kg) and wither-height (SH = 89.5 ± 0.3, SHF = 90.1 ± 0.3 cm) on d 68. Concentrations of metabolites were not affected by treatment. Cooling the environment through fans reduced RT and the risk of hyperthermia at 1000 h but it did not affect performance of pre-weaned Holstein calves.

Heat abatement during the pre-weaning period: effects on lying behavior and disbudding-related responses of male Holstein calves.

Strategies to abate heat stress are seldom adopted for pre-weaned dairy calves and little is known about their effects on behavior and pain sensitivity of youngstock. Our objectives were to determine the effects of heat stress abatement on lying behavior and disbudding-related pain sensitivity, wound healing, and change in intake. Male Holstein calves (n = 60; 0 to 68 d of age) were assigned randomly at birth (d 0) to 1 of 3 treatments: hutch outdoors with 50% of its area covered with plywood (control = 20), hutch in a barn with no cooling (SH = 21), and hutch in a barn with ceiling fans (SHF = 19). Calves were fitted with lying-behavior loggers on the hind leg from d 1 to 68. On d 32 ± 8 (±SD), we disbudded calves using hot iron, 30 min after cornual nerves were blocked with lidocaine. Immediately before (0 h), and at 1, 2, and 3 h after disbudding, we evaluated calves for mechanical nociceptive threshold (MNT) and head (ear flick, head shake, head rubbing) and somatic (tail flicking, foot stamping, restlessness) behaviors. On d 1, 3, 7, and 14 after disbudding, we evaluated the MNT and, on d 7 and 14, we evaluated wound healing (1 = crust, 5 = exudate). We calculated the relative change in milk solids and starter intake from d 0 to 6 relative to disbudding compared with the average of the 72 h preceding the procedure. The lying time was 0.6 h/d greater for the SHF treatment compared with the SH treatment. The control treatment resulted in 3.2 and 4.1 more lying bouts per day than the SH and SHF treatments, respectively; consequently, the control treatment resulted in lying-bout duration 7.7 and 10.9 min/event shorter than the SH and SHF treatments, respectively. We did not detect an effect of treatment on the number of disbudding-related head and somatic behaviors and MNT. The odds of calves having abnormal wound was 3.5 and 3.2 times greater for the control treatment compared with the SH and SHF treatments, respectively. We did not detect an effect of treatment on the relative change in intake of milk solids and starter. Heat abatement improves the welfare of pre-weaned dairy calves and may hasten healing.

Heat abatement during the pre-weaning period: effects on growth, feed efficiency, metabolites, and insulin of male Holstein calves.

Heat stress abatement strategies for pre-weaned dairy calves are seldom evaluated. An experiment was conducted to evaluate the effects of housing calves under a barn and provision of fans to calves housed under a barn on calfhood performance. The experiment was conducted in a dairy in southern Georgia, USA. Male Holstein calves (n = 60; 0 to 68 day of age) were assigned randomly at birth (day 0) to 1 of 3 treatments: hutch outdoors with 50% of its area covered with plywood (control = 20), hutch in a barn with no cooling (SH = 21), and hutch in a barn with ceiling fans (SHF = 19). Body weight (BW) was measured at birth, and total serum protein and wither-height were measured 24 to 48 h after birth. A sub-set of hutches was evaluated for air speed and temperature, and rectal temperature (RT) and respiratory frequency (RF) of calves housed in these hutches were measured at 0900 and 1500 h. Intakes of liquid feed (days 14 to 63) and starter (days 14 to 68) were recorded daily, BW and wither-height were measured weekly, and feed efficiency was calculated weekly. Blood was sampled on days 1, 14, 28, 42, 49, 52, 56, 58, 63, and 65 for the measurement of fatty acids, ß-hydroxybutyrate, glucose, and insulin. The SHF treatment resulted in air velocity 0.56 to 0.83 m/s greater (P < 0.01) than the control and SH treatments, respectively, whereas the control treatment resulted in air temperature 1.2 to 3.2 °C greater (P < 0.01) than the SH and SHF treatments, respectively. The RT of calves in the control treatment was 0.1 to 1.1 °C greater (P ≤ 0.03) than the SH and SHF treatments, respectively, and the control treatment resulted in RF 39.4 to 60.2 mov/min greater (P < 0.01) than the SH and SHF treatments, respectively. Treatment did not (P ≥ 0.27) affect feed efficiency and concentrations of metabolites and insulin, but calves in the control treatment were 2.6 cm shorter (P = 0.03) than calves in the SHF treatments at weaning. Provision of fans to calves housed under a barn reduced RT, RF, but only had a minute impact on wither-height.