Identification of proteins with increased levels in ameloblastic carcinoma.

PURPOSE: The comparative proteomic approach by a combination of 2-dimensional electrophoresis and matrix-assisted laser desorption-ionization-time of flight mass spectrometry (MS) analysis is an attractive strategy for the discovery of cancer biomarkers and therapeutic targets. The identification of protein biomarkers associated with ameloblastic carcinoma (AC), a malignant epithelial odontogenic tumor, will potentially improve the diagnostic and prognostic accuracy for this malignant neoplasm. The aim of the present study was to identify highly expressed proteins in AC that could be considered as potential biomarkers. MATERIALS AND METHODS: The protein profile of an AC was compared with the protein profiles of 3 cases of benign ameloblastoma. Proteins that showed increased levels in AC were identified using MS, and the augmented amount of some of these proteins in the malignant lesion was confirmed by Western blot or immunohistochemistry. RESULTS: We detected a total of 782 spots in the protein profile of AC, and 19 of them, showing elevated levels compared with benign ameloblastoma, were identified using MS. These proteins have been implicated in several cellular functions, such as cell structure, metabolism, stress response, and signal transduction. CONCLUSIONS: The increased expression of the identified proteins and the minor expression of some proteins that might inhibit tumor progression could be involved in the evolution from a benign lesion to carcinoma.

Patología oral en un grupo de pacientes mejicanos con enfermedades genéticas / Oral pathology in a group of Mexican patients with genetic diseases

Dejando de lado la patología infecciosa y traumática, la gran mayoría de las patologías odontológicas, tienen una base genética, en algunas de ellas identificada, en otras no. Para los estomatólogos es de gran importancia conocer las característicasclínicas y el tipo de alteración que acompañan a los síndromes de etiología genética, para poder ofrecer a los pacientes un tratamiento apropiado y multidisciplinario.Objetivo: Búsqueda intencional y descripción de la patología bucal en pacientes con diversas enfermedades genéticas.Diseño del estudio: Se realizó un estudio observacional y descriptivo reuniendo a 62 pacientes de la consulta de Genética del Instituto Nacional de Rehabilitación durante 4 meses. Se tomaron en cuenta, además de las manifestaciones bucales y la enfermedad genética relacionada a las mismas, el sexo, la edad, la presencia o no de consanguinidad, y endogamia, así como la localización de la manifestación bucal. La mayoría de los pacientes que presentan patología genética no tienen tratamiento curativo, pero sí podrán realizarse otros tratamientos para mejorar su calidad de vida, entre estos se cuentan los diversos tratamientos odontológicos.Resultados: Los padecimientos diagnosticados con mayor frecuencia fueron neuropatías periféricas hereditarias, displasias esqueléticas, malformaciones de miembros y distrofias musculares. Se describieron diversas manifestaciones que fueron registradas y agrupadas de acuerdo a su localización

Without considering infectious and traumatic diseases, the great majority of oral cavity diseases have a genetic base, in some cases identifiable, in others not. For the stomatologists it is of great importance to know the clinical characteristics and type of alteration that go with genetic etiology syndromes to be able to offer patients an adequate multidisciplinary treatment.Objective: Intentional search and description of oral pathology in patients with diverse genetic diseases.Study design: An observational and descriptive 4 month study of 62 patients from the Genetics Department of the NationalInstitute of Rehabilitation, was done. Taken into consideration, aside from oral manifestations and genetic disease, were age, sex, consanguinity and inbreeding. The majority of patients who have genetic pathology do not have curative treatment, but they can receive other treatments to improve their quality of life, among these are dental treatments.Results: The more common diseases we found were hereditary peripheral neuropathies, skeletal dysplasias, limb malformationand muscular dystrophies. Diverse features were described, registered and grouped according to their location.Conclusions: Presently it is important to look for the genetic etiology of all diseases to seek specific treatments and prevent them. This will change the practice of medicine and dentistry

Oral pathology in a group of Mexican patients with genetic diseases.

UNLABELLED: Without considering infectious and traumatic diseases, the great majority of oral cavity diseases have a genetic base, in some cases identifiable, in others not. For the stomatologists it is of great importance to know the clinical characteristics and type of alteration that go with genetic etiology syndromes to be able to offer patients an adequate multidisciplinary treatment. OBJECTIVE: Intentional search and description of oral pathology in patients with diverse genetic diseases. STUDY DESIGN: An observational and descriptive 4 month study of 62 patients from the Genetics Department of the National Institute of Rehabilitation, was done. Taken into consideration, aside from oral manifestations and genetic disease, were age, sex, consanguinity and inbreeding. The majority of patients who have genetic pathology do not have curative treatment, but they can receive other treatments to improve their quality of life, among these are dental treatments. RESULTS: The more common diseases we found were hereditary peripheral neuropathies, skeletal dysplasias, limb malformation and muscular dystrophies. Diverse features were described, registered and grouped according to their location. CONCLUSIONS: Presently it is important to look for the genetic etiology of all diseases to seek specific treatments and prevent them. This will change the practice of medicine and dentistry.