Assessment of environmental forensic indicator for anthropogenic groundwater contamination via target/suspect/nontarget analysis using HRMS techniques.

This study compared target/suspect/nontarget analysis via liquid chromatography-high-resolution mass spectrometry (LC-HRMS) with traditional environmental forensic methods, specifically nitrate and its stable N isotope, in assessing groundwater pollution from livestock manure and agriculture. Using an in-house database of 1471 target and suspects, 35 contaminants (pesticides, veterinary drugs, surfactants) were identified, some uniquely linked to specific pollution sources, such as sulfamethazine and 4-formylaminoantipyrine in manure-affected areas. Pesticides were widespread, typically showing higher intensity in agricultural zones. On the other hand, the results of stable N isotope analysis (δ15N-NO3: 4.8 to 16.4‰) indicated the influence of human activities such as fertilizers, sewage, and manure in all sampling sites, including the control site far from the pollution sources and cannot differentiate the specific sources. The study underscores LC-HRMS's efficacy in different pollution sources, surpassing the limitations of stable N isotope analysis, and provides valuable insights for polluted groundwater source tracking strategies.

Simultaneous multi-residue analytical method for anesthetics and sedatives in seafood samples by LC-ESI/MSMS.

A simultaneous multi-residue analytical method for 27 regulated and unregulated anesthetics and sedatives in seafood using liquid chromatography/tandem mass spectrometry with electrospray ionization (LC-ESI/MSMS) was developed and tested on flatfish, eels, and shrimp. To optimize the efficiency of the method, extraction and clean-up procedures with various solvents and sorbents were tested. The most efficient pretreatment methods were extraction using acetonitrile (ACN) only (for flatfish and eel) and 0.1 % ammonium acetate in ACN (for shrimp). Validation was performed under the guidelines of CODEX Alimentarius (CAC/GL-71) and the Korean National Institute of Food and Drug Safety Evaluation (NIFDS). The limit of detection (LOD), limit of quantification (LOQ), accuracy, and precision for all compounds ranged from 0.0002 to 0.002 mg/kg, 0.0005-0.005 mg/kg, 64.7-112.5 %, and 1.0-8.6 %, respectively. The coefficient of determination (linearity, R2) was over 0.98. Therefore, the method meets the requirements of both the domestic and international guidelines.

Dietary exposure to PCBs by seafood cooking method: A Korean study.

The levels of 82 polychlorinated biphenyl (PCB) congeners in 86 different types of seafood (n = 237) were analyzed and the PCBs changes in seafood by seven cooking methods were verified from 51 different species of seafood (total 127 pairs) to confirm the effect of cooking on dietary PCB levels in the human body. Total PCB levels in raw seafood ranged from 0.01 to 20.6 ng/g ww, while those for DL PCBs ranged from 0.001 to 1.67 pg TEQ/g ww. There was no statistically significant difference between PCB levels in raw and each different cooked seafood. However, Raw seafood samples with PCB concentrations under 1 ng/g showed a markedly increase in PCBs after cooking. When comparing mean and median value for total PCBs, the high-fat fish group exhibited a slightly decreasing PCB concentrations trend after cooking. The estimated daily intake (EDI) of cooked seafood was 1.07 ng/kg BW/day, while the EDI for raw seafood was 1.26 ng/kg BW/day.