ABSTRACT
An extraction process is reported that employs a near-supercritical mixture of CO2 and MeOH to extract the cardiac glycoside, digoxin, from the Digitalis lanata leaf. The method development of the sample preparation procedure is presented in detail, and reasons for trends that occur in the natural products extraction are given.
Subject(s)
Acetyldigoxins/analysis , Digitalis/chemistry , Digoxin/analysis , Plant Leaves/chemistry , Plants, Medicinal , Plants, Toxic , Acetyldigoxins/isolation & purification , Chromatography, High Pressure Liquid , Digoxin/isolation & purification , Hydrolysis , Plant Extracts/analysisABSTRACT
It has been shown that inverse supercritical fluid extraction (SFE) can be used to analytically isolate a polar analyte from its matrix even at low concentrations (0.016%). Inverse SFE has been shown to be successful not only for the semi-solid Neosporin ointments, but also for the semi-liquid Neosporin creams as well. The technique has been shown to be superior to solid-phase extraction in both cases and affords the analytical chemist a quicker and safer method of sample preparation of analytes from both creams and ointments.
Subject(s)
Ointments/analysis , Pharmaceutical Preparations/analysis , Chemical Phenomena , Chemistry, Physical , Chromatography, High Pressure Liquid , Polymyxin B/analysis , TemperatureABSTRACT
Supercritical fluid (SF) CO2 is receiving a great deal of interest in the scientific and engineering community as a replacement for toxic organic solvents. Analytical chemists employ large quantities of organic solvents during preparation of the sample for analysis. The application of SF extraction with CO2 and modified CO2 to the isolation of active drug components and metabolites from various pharmaceutical and biological matrices is reviewed. Studies are described that deal with spiked drugs in animal feed, residual solvent in drug formulations, and active ingredients in over-the-counter products. The experimental challenges to implementing this technology for trace analysis are discussed. While much of the impetus for working with SFs is prompted by regulatory issues, it would appear that SFs afford the analyst a better-cheaper-faster-safer way of performing drug analysis.
