ABSTRACT
OBJECTIVE: Evaluate the effect of ionizing irradiation by 60Co on Vibrio cholerae O1, El-Tor, Ogawa, non-toxigenic, incorporated in live oysters Crassostrea brasiliana. METHODS: Samples of oysters were selected from Cananéia town in the South coast of S. Paulo state, Brazil, contaminated with Vibrio cholerae and irradiated with 60Co at 0.5 and 1.0 kGy dosages. RESULTS: Showed significant reductions of the initial number of V. cholerae, ranging from 3.4 x10(7) to 10(3) and 10(2), respectively. The D10 values related with the respective doses of irradiation were 0.173 and 0.235. CONCLUSION: Considering a 6 value as safety factor, it is concluded that 1.41 kGy irradiation dosage is necessary to eliminate a high number of V. cholerae viable cells in oysters. Controls were used in the experiment.
Subject(s)
Food Irradiation , Ostreidae/microbiology , Radiation Tolerance , Shellfish/microbiology , Vibrio cholerae/radiation effects , Animals , Food Microbiology , Radiation DosageABSTRACT
A presença de certos microrganismos nos alimentos ou de metabolitos originados durante sua multiplicaçäo pode dar lugar a várias enfermidades no homem: as intoxicaçöes e as infecçöes alimentares. Dentre os agentes causadores das Enfermidades Transmitidas por Alimentos encontra-se o Vibrio cholerae 01. Estudou-se no presente trabalho a radiossensibilidade in vitro de quatro cepas de V cholerae 01, submetidas ao tratamento com diferentes doses de radiaçäo ionizante de 60 sobrescrito Co, e compararam-se os resultados obtidos com dados da literatura relacionados a problemas de veiculaçäo alimentar de bactérias patogênicas, incluindo a água
