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1.
Int J Oral Maxillofac Surg ; 49(11): 1459-1463, 2020 Nov.
Article in English | MEDLINE | ID: mdl-32278624

ABSTRACT

Temporomandibular joint (TMJ) arthrocentesis is considered an effective and minimally invasive procedure for certain conditions related to temporomandibular disorders. The ideal irrigation volume for arthrocentesis lavage has not yet been defined. Therefore, the aim of this study was to evaluate the efficacy of different saline solution volumes in removing methylene blue from the TMJ space of fresh human cadavers. Nineteen cadavers were selected and 1ml of 10µM methylene blue solution was injected into the upper joint space unilaterally. Conventional arthrocentesis was then conducted by infusion of 300ml of 0.9% saline solution, collecting a 1-ml sample from the drained quantity for every 25ml injected. Finally, the samples were assayed by measuring photo absorbance of the methylene blue solution. There was a statistically significant difference between the irrigation volumes regarding the removal of methylene blue solution from the joint space (P<0.001), specifically between the first 25 ml and 200 ml (P=0.014), 225 ml (P=0.001), 250 ml (P<0.001), and 275 ml (P=0.001). Based on this ex vivo study, a 25-ml perfusion volume appears to be sufficient for joint lavage in conventional arthrocentesis of the TMJ.


Subject(s)
Arthrocentesis , Temporomandibular Joint Disorders , Cadaver , Humans , Range of Motion, Articular , Temporomandibular Joint/surgery , Temporomandibular Joint Disorders/surgery , Therapeutic Irrigation , Treatment Outcome
2.
Morphologie ; 104(344): 59-63, 2020 Mar.
Article in English | MEDLINE | ID: mdl-31587840

ABSTRACT

PURPOSE: To investigate the relationship between the mandibular foramen (MF) and the inferior occlusal plane (IOP) of anatomic specimens, in order to guide clinicians in the inferior alveolar nerve block (IANB). METHOD: Eighty-two dry mandibles (totally or partially toothed) of Brazilian adults were selected, totalizing 66 left and 66 right antimeres. A flat plate of rigid PVC plastic was placed on the occlusal surfaces of posterior teeth and pressure was then manually applied to simulate the IOP. Following, the perpendicular distance from the IOP to the lowest point of the MF was measured by the use of a digital caliper, adopting negative values inferiorly and positive values superiorly. RESULTS: Regarding the antimeres, the majority of the lowest limits of the MF was below the IOP (right: 86.4%, left: 81.8%) with a mean of -4.2mm (±4.8) in the right and -4.1mm (±4.8) in the left ones, without any statistical significance. Considering both sexes, no statistically significant difference was obtained between them. CONCLUSION: The IOP seems not to be a good anatomical landmark to localize the MF and, its turn, to guide clinicians during the IANB for Brazilian adults, given the marked topographic variations.


Subject(s)
Anatomic Landmarks , Mandible/innervation , Mandibular Nerve/anatomy & histology , Nerve Block/methods , Adult , Anesthetics, Local/administration & dosage , Brazil , Female , Humans , Injections/methods , Male , Mandibular Nerve/drug effects , Middle Aged , Young Adult
3.
Forensic Sci Int ; 289: 449.e1-449.e6, 2018 Aug.
Article in English | MEDLINE | ID: mdl-29921482

ABSTRACT

This short communication aims to inform the scientific community of the existence of seven identified osteological collections in Brazil, most of which are housed in universities: 21st Century Collection of the Center for Studies in Forensic Anthropology, Recife; Identified Skeletal Collection of Sergipanos of the University Tiradentes, Aracaju; Identified Skull Collection of the State of Bahia, Aracaju; Osteological and Tomographic Collection - Prof. Dr. Eduardo Daruge, Piracicaba; Osteological Collection of the Institute of Teaching and Research in Forensic Sciences, Guarulhos; Identified Skull Collection of the Anatomy Museum Alfonso Bovero, São Paulo; and the Identified Skull Collection, São Paulo. Three of these collections are from a northeast population, whereas the others are from individuals from the southeast region. Altogether, there are 925 skeletons and more than 998 skulls (three of the referenced collections have only skulls). Data on the number of individuals, sex, age, origin, and dates of inhumation and exhumation are available. This large quantity of identified skeletal remains is a fundamental source of research material that can be used to characterize the Brazilian population and facilitate the development of forensic anthropology. When contextualized within the reference series from South and Central America, it is clear that the Brazilian skeletal reference series hold a position of prominence.


Subject(s)
Bone and Bones , Forensic Anthropology , Adolescent , Adult , Aged , Aged, 80 and over , Brazil , Child , Child, Preschool , Female , History, 20th Century , History, 21st Century , Humans , Infant , Infant, Newborn , Male , Middle Aged , Research , Universities , Young Adult
4.
Eur J Histochem ; 55(3): e24, 2011.
Article in English | MEDLINE | ID: mdl-22073371

ABSTRACT

The objective was to study the morphology of the articular disc and analyse the immunohistochemical expression of types I and III collagen markers in the temporomandibular joint (TMJ) disc of human foetuses of different gestational ages. Twenty TMJ from human foetuses supplied by Universidade Federal de Uberaba with gestational ages from 17 to 24 weeks were studied. The gestational age of the foetuses was determined by measuring the crown-rump (CR) length. Macroscopically, the foetuses were fixed in 10% formalin solution and dissected by removing the skin and subcutaneous tissue and exposing the deep structures. Immunohistochemical markers of type I and III were used to characterize the existence of collagen fibres. Analysis of the immunohistochemical markers of types I and III collagen revealed the presence of heterotypical fibril networks.


Subject(s)
Collagen Type II/biosynthesis , Collagen Type I/biosynthesis , Fetus/embryology , Gene Expression Regulation, Developmental/physiology , Temporomandibular Joint Disc/embryology , Female , Fetus/cytology , Gestational Age , Humans , Immunohistochemistry , Male , Temporomandibular Joint Disc/cytology
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