ABSTRACT
The Antarctic soil microbial community has a crucial role in the growth and stabilization of higher organisms, such as vascular plants. Analysis of the soil microbiota composition in that extreme environmental condition is crucial to understand the ecological importance and biotechnological potential. We evaluated the efficiency of isolation and abundance of strict anaerobes in the vascular plant Deschampsia antarctica rhizosphere collected in the Antarctic's Admiralty Bay and associated biodiversity to metabolic perspective and enzymatic activity. Using anaerobic cultivation methods, we identified and isolated a range of microbial taxa whose abundance was associated with Plant Growth-Promoting Bacteria (PGPB) and presences were exclusively endemic to the Antarctic continent. Firmicutes was the most abundant phylum (73 %), with the genus Clostridium found as the most isolated taxa. Here, we describe two soil treatments (oxygen gradient and heat shock) and 27 physicochemical culture conditions were able to increase the diversity of anaerobic bacteria isolates. Heat shock treatment allowed to isolate a high percentage of new species (63.63 %), as well as isolation of species with high enzymatic activity (80.77 %), which would have potential industry application. Our findings contribute to the understanding of the role of anaerobic microbes regarding ecology, evolutionary, and biotechnological features essential to the Antarctic ecosystem.
Subject(s)
Bacteria, Anaerobic/isolation & purification , Industrial Microbiology , Microbiota , Poaceae/microbiology , Rhizosphere , Adaptation, Physiological , Antarctic Regions , Bacteria, Anaerobic/classification , Bacteria, Anaerobic/enzymology , Bacteria, Anaerobic/genetics , Cold Temperature , Soil MicrobiologyABSTRACT
Fusobacterium nucleatum is a Gram-negative, non-spore-forming, nonmotile, obligatory anaerobic rod that is normally isolated from the oral cavity. Epidemiological studies have shown that this species is one of the most prevalent in primary root canal infections. The purpose of this study was to compare the effectiveness of bacteriological culture, 16S rDNA directed polymerase chain reaction and checkerboard DNA-DNA hybridization for detection of F. nucleatum strains in infected teeth associated with periradicular lesions. Thirteen single-root teeth from adult patients, all having carious lesions, necrotic pulps, and radiographic evidence of periradicular bone loss were included in this study. Combining all methods, the results indicated that F. nucleatum was present in approximately 31% (4 of 13) of the specimens. Incidence of F. nucleatum in root canal infections, as evaluated in this study by polymerase chain reaction, culture, and DNA-DNA hybridization, was 15.4%, 15.4%, and 10.0%, respectively. Our data demonstrated that no method used herein could be considered superior for detecting F. nucleatum directly from clinical samples. However, the small number of samples examined and the low prevalence that was observed should be considered.
Subject(s)
Bacterial Typing Techniques , Dental Pulp Necrosis/microbiology , Fusobacterium Infections/microbiology , Fusobacterium nucleatum/isolation & purification , Periapical Periodontitis/microbiology , Adult , Colony Count, Microbial , DNA, Bacterial/analysis , Fusobacterium nucleatum/genetics , Humans , Nucleic Acid Hybridization/methods , Polymerase Chain Reaction/methodsABSTRACT
O hipoclorito de sódio (NaOCI) é uma das substâncias mais utilizadas em Endodontia durante a irrigaçäo de canais radiculares. As águas sanitárias, que contêm o NaOCI em uma concentraçäo variando entre 2-4 por cento, consistem em uma alternativa para as soluçöes de NaOCI disponíveis no comércio odontológico. Este estudo avaliou a atividade antimicrobiana de sete marcas nacionais de água sanitária, por meio do teste de difusäo em ágar. Os resultados demonstraram que todos os produtos testados apresentaram eficácia antimicrobiana
