ABSTRACT
INTRODUCTION: Group B streptococci (GBS) are transmitted from the mother to the newborn. Prevention of neonatal infection is achieved by intrapartum prophylaxis given to mothers colonized with GBS at 35 to 37 weeks of pregnancy. MATERIALS AND METHODS: Liquid biphasic Granada medium (LB) and instant liquid biphasic Granada medium (ILB) were evaluated for GBS detection. Vaginal swabs obtained from 300 women were inoculated onto LB or ILB, or onto Todd-Hewitt broth and analyzed with the ATB system (comparison method). RESULTS: Prevalence of GBS was 20% (61/300). LB and Todd-Hewitt with ATB detected GBS in 20% of women, and ILB in 19% of women. No growth was observed at four hours in any of the media studied. At 10 h and 14 h, identification of GBS was possible in 43/300 (14%) and 53/300 (18%) of ILB cultures, respectively, and in 32/300 (11%) and 46/300 (15%) of LB cultures. CONCLUSION: All the media used are suitable for GBS detection. The majority of GBS were identified in ILB and LB cultures at 10 h and 14 h.
Subject(s)
Culture Media/pharmacology , Streptococcal Infections/microbiology , Streptococcus agalactiae/drug effects , Adult , Female , Humans , Infant, Newborn , Infectious Disease Transmission, Vertical/prevention & control , Pregnancy , Pregnancy Complications, Infectious/diagnosis , Pregnancy Complications, Infectious/epidemiology , Pregnancy Complications, Infectious/microbiology , Rectum/microbiology , Streptococcal Infections/diagnosis , Streptococcal Infections/epidemiology , Streptococcal Infections/prevention & control , Streptococcal Infections/transmission , Streptococcus agalactiae/growth & development , Streptococcus agalactiae/isolation & purification , Vagina/microbiologyABSTRACT
Introduction. Group B streptococci (GBS) are transmitted from the mother to the newborn. Prevention of neonatal infection is achieved by intrapartum prophylaxis given to mothers colonized with GBS at 35 to 37 weeks of pregnancy. Materials and methods. Liquid biphasic Granada medium (LB) and instant liquid biphasic Granada medium (ILB) were evaluated for GBS detection. Vaginal swabs obtained from 300 women were inoculated onto LB or ILB, or onto Todd-Hewitt broth and analyzed with the ATB system (comparison method). Results. Prevalence of GBS was 20% (61/300). LB and Todd-Hewitt with ATB detected GBS in 20% of women, and ILB in 19% of women. No growth was observed at four hours in any of the media studied. At 10 h and 14 h, identification of GBS was possible in 43/300 (14%) and 53/300 (18%) of ILB cultures, respectively, and in 32/300 (11%) and 46/300 (15%) of LB cultures. Conclusion. All the media used are suitable for GBS detection. The majority of GBS were identified in ILB and LB cultures at 10 h and 14 h (AU)
Introducción Los estreptococos del grupo B (SGB) se transmiten de la madre al recién nacido. La prevención de la infección neonatal se logra mediante la profilaxis intraparto de las madres colonizadas por SGB en las semanas 35 a 37 de gestación. Materiales y métodos Se ha evaluado el uso del medio Granada líquido bifásico (LB) y del medio Granada líquidobifásico instantáneo (LBI) para la detección de SGB. Se tomaron muestras vaginales con torunda de 300 mujeres, se inocularon las torundas en LB, LBI o en caldo Todd-Hewitt, y se analizaron los resultados con el sistema ATB (método de comparación). Resultados La prevalencia de SGB fue del 20% (61/300). Los medios LB y Todd-Hewitt con ATB detectaron SGB en el 20% de mujeres, y el medio LBI en el 19%. No se observó crecimiento a las 4 h en ninguno de los medios estudiados. A las 10 y 14 h se pudo identificar SGB en 43/300 (14%) y en 53/300 (18%) de los cultivos en LBI, respectivamente, y en 32/300 (11%) y en 46/300 (15%) de los cultivos en LB. Conclusión Todos los medios usados son adecuados para la detección de SGB. La mayoría de SGB se identificaron en LBI y en LB a las 10 y a las 14 (AU)
