Correction: Changes in latent fingermark glyceride composition as a function of sample age using UPLC-IMS-QToF-MSE.

Correction for 'Changes in latent fingermark glyceride composition as a function of sample age using UPLC-IMS-QToF-MSE' by Amanda A. Frick, et al., Analyst, 2020, DOI: .

Changes in latent fingermark glyceride composition as a function of sample age using UPLC-IMS-QToF-MSE.

The composition of fingermark residue has been an important topic in forensic science, mainly in efforts to better understand and eventually improve the efficacy of latent fingermark detection methods. While the lipid fraction has been extensively studied, there is currently little information about how the glyceride fraction of latent fingermarks is chemically altered over time following deposition. A previously reported untargeted ultra performance liquid chromatography-ion mobility spectrometry-quadrupole time-of-flight mass spectrometry (UPLC-IMS-QToF-MSE) method was used to investigate changes over time in fingermark di- and triglycerides. Charged latent fingermark samples from 5 donors were analysed up to 28 days following deposition. Significant changes in glyceride composition occurred with increased sample age, attributed primarily to the oxidation of unsaturated triglycerides through ozonolysis. Considerably fewer unsaturated TGs were identified in samples 7 and 28 days following deposition, while mono- and diozonides of these lipids were identified as major components of aged samples. Additional compounds were identified as possible aldehyde and carboxylic acid derivatives resulting from the reaction of water with ozonolysis intermediates. While the onset of these processes occurred rapidly following deposition, continuing oxidation over time was seen via the progressive ozonolysis of diunsaturated triglycerides. These results represent a further step towards understanding the factors affecting fingermark composition, ageing and subsequent detection under operational conditions.

Sara phosphorylation state controls the dispatch of endosomes from the central spindle during asymmetric division.

During asymmetric division, fate assignation in daughter cells is mediated by the partition of determinants from the mother. In the fly sensory organ precursor cell, Notch signalling partitions into the pIIa daughter. Notch and its ligand Delta are endocytosed into Sara endosomes in the mother cell and they are first targeted to the central spindle, where they get distributed asymmetrically to finally be dispatched to pIIa. While the processes of endosomal targeting and asymmetry are starting to be understood, the machineries implicated in the final dispatch to pIIa are unknown. We show that Sara binds the PP1c phosphatase and its regulator Sds22. Sara phosphorylation on three specific sites functions as a switch for the dispatch: if not phosphorylated, endosomes are targeted to the spindle and upon phosphorylation of Sara, endosomes detach from the spindle during pIIa targeting.

Uninflatable and Notch control the targeting of Sara endosomes during asymmetric division.

Cell fate decision during asymmetric division is mediated by the biased partition of cell fate determinants during mitosis [1-6]. In the case of the asymmetric division of the fly sensory organ precursor cells, directed Notch signaling from pIIb to the pIIa daughter endows pIIa with its distinct fate [1-6]. We have previously shown that Notch/Delta molecules internalized in the mother cell traffic through Sara endosomes and are directed to the pIIa daughter [6]. Here we show that the receptor Notch itself is required during the asymmetric targeting of the Sara endosomes to pIIa. Notch binds Uninflatable, and both traffic together through Sara endosomes, which is essential to direct asymmetric endosomes motility and Notch-dependent cell fate assignation. Our data uncover a part of the core machinery required for the asymmetric motility of a vesicular structure that is essential for the directed dispatch of Notch signaling molecules during asymmetric mitosis.

Protocolo de intervención en miniresidencias / No disponible

El protocolo recoge los criterios fundamentales en base a los cuales se ha diseñado la intervención en este tipo de recurso, así como las áreas, programas y talleres mediante los que dicha intervención se lleva a cabo. Para ello se ha considerado que las miniresidencias son recursos de rehabilitación en los que la persona ha de ir progresando en los objetivos relacionados con su competencia personal, social y su funcionamiento ocupacional, sin perder la perspectiva de que es un lugar en el que van a llevar a cabo su vida cotidiana. Se ha pretendido elaborar un diseño que favorezca que las personas que acceden al recurso, puedan llevara cabo actividades y tareas que les permitan participar en aquellos roles que configuran su identidad, sentirse útiles e integrados socialmente y tener una vida gratificante y de calidad (AU)

The protocol develops the main criteria on which the design of the intervention in this type of centre is based, as well as the areas, programs and workshops held to carry out the intervention. For this purpose, it has been considered that ‘miniresidencias’ (Group Homes) are rehabilitation resources where the person has to keep on progressing in the goals related to his personal and social competence and his occupational performance, without loosing the perspective that it is a place where they are going to carryout their daily life. It has been intended to develop a design to enable the people who access the resource to be able to carry out activities and tasks which allow them to participate in those roles which form their identity, to feel useful and socially integrated and to have a satisfying quality of life (AU)