ABSTRACT
BACKGROUND: BioCen-128 is a new active pharmaceutical ingredient composed of a specific bovine thymic fraction of a polypeptide nature. Positive results of similar thymus extracts have been shown to be effective in delaying the processes associated with aging, immunosenescence and Alzheimer's disease (AD), where the inflammation plays an important role. Because of the anti-inflammatory potential of BioCen-128, the aim of this study was to evaluate the granuloma model induced by a cotton wool implantation and the model induced by intracerebroventricular (ICV) administration of streptozotocin (STZ). METHOD: The experiment was carried out using male OF-1cenp mice weighing 20 ± 2 g. RESULTS: Mice administered BioCen-128 in via the IP route at 5, 10 and 20 mg/kg of corporal weigh showed a decrease in the wet and dry weights of the granuloma, providing evidence of a systemic anti-inflammatory effect. In the ICV model of STZ, the administration of BioCen-128 improved cognitive function. CONCLUSION: These responses suggested an anti-neuroinflammatory effect explainable by the action of thymosin ß4 and thymosin alfa proteins. The results suggested that BioCen-128 could be used in the prevention and treatment of some diseases, for example AD, where neuroinflammation is one of the biological events that take place.
Subject(s)
Alzheimer Disease , Alzheimer Disease/drug therapy , Animals , Cattle , Cognition , Disease Models, Animal , Granuloma/drug therapy , Male , Mice , StreptozocinABSTRACT
La historia es una disciplina que contribuye a la enfermería para dejar huella de la trascendencia de su quehacer profesional, el cuidado. La estrecha vinculación con el tiempo nos remite a Martin Heidegger como el filósofo que plantea una articulación inseparable entre el tiempo y el ser a través del cuidado. Por lo que se reflexiona sobre el contexto filosófico del cuidado y su relación con el tiempo, que permite identificar una paradoja en el quehacer profesional de enfermería, quienes se encuentran cumpliendo horarios, rutinas y actividades fuera de la cuestión ética de otorgar un momento para cada cosa, afectado por aceleradas dinámicas, sobrepoblación de pacientes en las instituciones de salud, haciendo imposible proporcionar el cuidado como tal, ya que su temporalidad consiste en satisfacer las necesidades para la existencia de la persona a partir de cuidados que permitan conceptualizarlo como un ser completo y en ese sentido, contar con los tiempos necesarios para poder realizar de manera satisfactoria un cuidado con calidad y fomentar el proceso de la construcción de la disciplina (AU)
History is a discipline that contributes to the nursing to trace the significance of their professional work, caring. The close relationship with time reminds us of Martin Heidegger as the philosopher who raises an inseparable articulation between time and the being through caring. As we reflect on the philosophical context of caring and its relation to time, which identifies a paradox in nursing professional work, who are serving schedules, routines and activities outside the ethical issue of giving one point for each thing affected by accelerated dynamics, overcrowding of patients in health institutions, making it impossible to provide care as such, as its temporality is to meet the needs for the existence of the person from allowing conceptualize care as a whole being and in that sense, have the time required to perform satisfactorily quality care and encourage the process the construction of discipline (AU)
A História é uma disciplina que contribui para a enfermaria para traçar o significado da sua vida profissional, atencioso. A estreita ligação com o tempo nos faz lembrar de Martin Heidegger como o filósofo que levanta uma ligação inseparável entre o tempo e sendo por carinho. Ao refletirmos sobre o contexto filosófico do cuidado e sua relação com o tempo, o que identifica um paradoxo em enfermagem do trabalho profissional, que estão servindo horários, rotinas e atividades fora a questão ética de dar um ponto para cada coisa afetada pela dinâmica acelerada, a superlotação de pacientes em instituições de saúde, o que torna impossível a prestação de cuidados como tal, sua temporalidade é para atender às necessidades para a existência da pessoa de permitir que o cuidado conceituar como um todo, sendo e, nesse sentido, têm o tempo necessário para realizar atendimento de forma satisfatória a qualidade e incentivar o processo de construção de disciplina (AU)
Subject(s)
Humans , History of Nursing , Nursing Care/psychology , Philosophy, Nursing , TimeABSTRACT
Beverages, especially wines are well-known to contain a variety of health-beneficial bioactive substances, mainly of phenolic nature which frequently exhibit antioxidant activity. Significant information is available about the separation and identification of polyphenols from some beverages by chromatographic and spectroscopic techniques, but considerably poor is chemical data related to the polyphenolic content in rums. In this paper, a method involving the all-liquid chromatographic technique of high-speed countercurrent chromatography (HSCCC) combined with high-performance liquid chromatography coupled with diode-array detection and electrospray ionization mass spectrometry (HPLC-DAD-ESI-MS(n)) has been successfully applied for separation and identification of phenolic compounds in an aged rum. Besides, the phenolic fraction (PF) was assayed for its antioxidant effects using three different free radical in vitro assays (DPPH·, RO(2)· and spontaneous lipid peroxidation (LPO) on brain homogenates) and on ferric reducing antioxidant power (FRAP). Results showed that PF potently scavenged DPPH and strongly scavenged peroxyl radicals compared to ascorbic acid and butylated hydroxytoluene (BHT); and almost equally inhibited LPO on brain homogenates subjected to spontaneous LPO when compared to quercetin. Moreover, PF also exhibited strong reducing power. This chemical analysis illustrates the rich array of phenols in the aged rum and represents a rapid and suitable method for the isolation and identification of phenolic compounds from mixtures of considerable complexity, achieving high purity and reproducibility with the use of two separation steps.
