Influence of shell thickness on the thermal stability and melting-like behavior of Al@Fe core-shell nanoparticles from atomistic simulations: a structural and dynamic description.

Core-shell nanoparticles (CSNPs) are a class of functional materials that have received important attention nowadays due to their adjustable properties by a controlled tuning of the core or shell. Understanding the thermal response and structural properties of these CSNPs is relevant to carrying out an analysis regarding their synthesis and application at the nanoscale. The present work is aimed to investigate the shell thickness effect on thermal stability and melting behavior of Al@Fe CSNPs by using molecular dynamics simulations. The results are discussed considering the influence of the Fe shell on the Al nanoparticle and analyzing the effect of different shell thicknesses in Al@Fe CSNPs. In general, calorific curves show a smooth energy decline for temperatures greater than room temperature for different shell thicknesses and sizes, corresponding to the inward and outward atomic movement of Al and Fe atoms, respectively, that produce a mixed Al-Fe nanoalloy. Here, the thermal stability of the Al@Fe nanoparticle is gradually lost passing to a liquid-Al@solid-Fe configuration and reaching a mixed Al-Fe state by an exothermic mechanism. Combining quantities of the atomic diffusion and structural identification, a stepped structural transition of the system is subsequently observed, where the melting-like point was estimated. Furthermore, it is observed that the Al@Fe CSNPs with greater stability are obtained with a thick shell and a large size. The ability to control shell thickness and vary the size opens up attractive opportunities to synthesize a broad range of new materials with tunable catalytic properties.

Genetic regulation of the ompX porin of Salmonella Typhimurium in response to hydrogen peroxide stress.

BACKGROUND: Salmonella Typhimurium is a Gram-negative pathogen that causes a systemic disease in mice resembling typhoid fever. During its infective cycle, S. Typhimurium is phagocytized by macrophages and proliferates inside a Salmonella-containing vacuole where Salmonella is exposed and survives oxidative stress induced by H2O2 through modulation of gene expression. After exposure of Salmonella to H2O2, the expression of the porin-encoding gene ompX increases, as previously shown by microarray analysis. Expression of ompX mRNA is regulated at a post-transcriptional level by MicA and CyaR sRNAs in aerobiosis. In addition, sequence analysis predicts a site for OxyS sRNA in ompX mRNA. RESULTS: In this work we sought to evaluate the transcriptional and post-transcriptional regulation of ompX under H2O2 stress. We demonstrate that ompX expression is induced at the transcriptional level in S. Typhimurium under such conditions. Unexpectedly, an increase in ompX gene transcript and promoter activity after challenges with H2O2 does not translate into increased protein levels in the wild-type strain, suggesting that ompX mRNA is also regulated at a post-transcriptional level, at least under oxidative stress. In silico gene sequence analysis predicted that sRNAs CyaR, MicA, and OxyS could regulate ompX mRNA levels. Using rifampicin to inhibit mRNA expression, we show that the sRNAs (MicA, CyaR and OxyS) and the sRNA:mRNA chaperone Hfq positively modulate ompX mRNA levels under H2O2-induced stress in Salmonella during the exponential growth phase in Lennox broth. CONCLUSIONS: Our results demonstrate that ompX mRNA is regulated in response to H2O2 by the sRNAs CyaR, MicA and OxyS is Salmonella Typhimurium.

Genetic regulation of the ompX porin of Salmonella Typhimurium in response to hydrogen peroxide stress

BACKGROUND: Salmonella Typhimurium is a Gram negative pathogen that causes a systemic disease in mice resembling typhoid fever. During its infective cycle, S. Typhimurium is phagocytized by macrophages and proliferates inside a Salmonella containing vacuole where Salmonella is exposed and survives oxidative stress induced by H2O2 through modulation of gene expression. After exposure of Salmonella to H2O2, the expression of the porin encoding gene ompX increases, as previously shown by microarray analysis. Expression of ompX mRNA is regulated at a post transcriptional level by MicA and CyaR sRNAs in aerobiosis. In addition, sequence analysis predicts a site for OxyS sRNA in ompX mRNA. RESULTS: In this work we sought to evaluate the transcriptional and post transcriptional regulation of ompX under H2O2 stress. We demonstrate that ompX expression is induced at the transcriptional level in S . Typhimurium under such conditions. Unexpectedly, an increase in ompX gene transcript and promoter activity after challenges with H2O2 does not translate into increased protein levels in the wild type strain, suggesting that ompX mRNA is also regulated at a post transcriptional level, at least under oxidative stress. In silico gene sequence analysis predicted that sRNAs CyaR, MicA, and OxyS could regulate ompX mRNA levels. Using rifampicin to inhibit mRNA expression, we show that the sRNAs (MicA, CyaR and OxyS) and the sRNA:mRNA chaperone Hfq positively modulate ompX mRNA levels under H2O2 induced stress in Salmonella during the exponential growth phase in Lennox broth. CONCLUSIONS: Our results demonstrate that ompX mRNA is regulated in response to H2O2 by the sRNAs CyaR, MicA and OxyS is Salmonella Typhimurium.

Eficacia de la viscosuplementación con ácido hialurónico de alto y de mediano peso molecular en pacientes con gonartrosis sintomática grados II-III / Efficacy of viscosupplementation with high and medium molecular weight hyaluronic acid in patients with symptomatic gonarthrosis grades II-III

Resumen: Introducción: La osteoartrosis es actualmente la quinta causa de discapacidad en el adulto. Su prevalencia aumenta a partir de los 40 años. La viscosuplementación es un tratamiento para las etapas iniciales de la osteoartrosis de rodilla. Objetivo: Comparar la eficacia y la calidad de vida en pacientes con gonartrosis sintomática tratados con ácido hialurónico de alto y mediano peso molecular. Material y métodos: Estudio analítico, retrospectivo, transversal, observacional de expedientes clínicos de pacientes con diagnóstico de gonartrosis grado II y grado III, de Octubre de 2017 a Junio de 2018, tratados con viscosuplementación con ácido hialurónico de alto o de mediano peso molecular; se analizó la calidad de vida y la escala de WOMAC de manera previa a la primera aplicación y con seguimiento a los dos, cuatro y seis meses. Resultados: 194 pacientes, con edades entre 30 y 87 años (promedio 61.85; DE = 11.3); 134 mujeres (69.1%) y 60 hombres (30.9%). En ambos grupos la evolución fue hacia la mejoría, con disminución en el puntaje WOMAC, sin diferencia significativa entre grupos. El puntaje de WOMAC tuvo un aumento mínimo pasados seis meses de la aplicación en ambos grupos, lo que se interpreta como disminución del efecto terapéutico. Dos pacientes tuvieron reacciones adversas mínimas localizadas, las cuales se resolvieron con medidas generales. Conclusión: El ácido hialurónico en ambas presentaciones, de alto y de bajo peso molecular, es efectivo en el tratamiento de la gonartrosis sintomática sin diferencia con el peso molecular.

Abstract: Introduction: OA is currently the fifth leading cause of adult disability, its prevalence increases from age 40. Viscosupplementation is a treatment for the early stages of knee OA. Objective: To compare efficacy and quality of life in patients with symptomatic gonartrosis, treated with high and low molecular weight hyaluronic acid. Material and methods: Analytical, retrospective, cross-sectional, observational study of clinical records of patients diagnosed with grade II and grade III gonarthrosis, October 2017 to June 2018, treated with viscosupplementation with high molecular weight hyaluronic acid or low molecular weight; WOMAC quality of life and scale were analyzed prior to the first application and tracked at two, four and six months. Results: 194 patients, aged between 30 to 87 years (promedio 61.85; SD = 11.3); 134 women (69.1%), 60 men (30.9%). In both groups the evolution was towards improvement, with a decrease in the WOMAC score, with no significant difference between groups. The WOMAC score had a minimum increase after 6 months of application in both groups, which is interpreted as decreasing the therapeutic effect. Two patients had localized minimal adverse reactions which were resolved with general measures. Conclusion: Hyaluronic acid in both presentations, high and low molecular weight, are effective in the treatment of symptomatic goonarthrosis without difference to molecular weight.

[Efficacy of viscosupplementation with high and medium molecular weight hyaluronic acid in patients with symptomatic gonarthrosis grades II-III]. / Eficacia de la viscosuplementación con ácido hialurónico de alto y de mediano peso molecular en pacientes con gonartrosis sintomática grados II-III.

INTRODUCTION: OA is currently the fifth leading cause of adult disability, its prevalence increases from age 40. Viscosupplementation is a treatment for the early stages of knee OA. OBJECTIVE: To compare efficacy and quality of life in patients with symptomatic gonartrosis, treated with high and low molecular weight hyaluronic acid. MATERIAL AND METHODS: Analytical, retrospective, cross-sectional, observational study of clinical records of patients diagnosed with grade II and grade III gonarthrosis, October 2017 to June 2018, treated with viscosupplementation with high molecular weight hyaluronic acid or low molecular weight; WOMAC quality of life and scale were analyzed prior to the first application and tracked at two, four and six months. RESULTS: 194 patients, aged between 30 to 87 years (promedio 61.85; SD = 11.3); 134 women (69.1%), 60 men (30.9%). In both groups the evolution was towards improvement, with a decrease in the WOMAC score, with no significant difference between groups. The WOMAC score had a minimum increase after 6 months of application in both groups, which is interpreted as decreasing the therapeutic effect. Two patients had localized minimal adverse reactions which were resolved with general measures. CONCLUSION: Hyaluronic acid in both presentations, high and low molecular weight, are effective in the treatment of symptomatic goonarthrosis without difference to molecular weight.

INTRODUCCIÓN: La osteoartrosis es actualmente la quinta causa de discapacidad en el adulto. Su prevalencia aumenta a partir de los 40 años. La viscosuplementación es un tratamiento para las etapas iniciales de la osteoartrosis de rodilla. OBJETIVO: Comparar la eficacia y la calidad de vida en pacientes con gonartrosis sintomática tratados con ácido hialurónico de alto y mediano peso molecular. MATERIAL Y MÉTODOS: Estudio analítico, retrospectivo, transversal, observacional de expedientes clínicos de pacientes con diagnóstico de gonartrosis grado II y grado III, de Octubre de 2017 a Junio de 2018, tratados con viscosuplementación con ácido hialurónico de alto o de mediano peso molecular; se analizó la calidad de vida y la escala de WOMAC de manera previa a la primera aplicación y con seguimiento a los dos, cuatro y seis meses. RESULTADOS: 194 pacientes, con edades entre 30 y 87 años (promedio 61.85; DE = 11.3); 134 mujeres (69.1%) y 60 hombres (30.9%). En ambos grupos la evolución fue hacia la mejoría, con disminución en el puntaje WOMAC, sin diferencia significativa entre grupos. El puntaje de WOMAC tuvo un aumento mínimo pasados seis meses de la aplicación en ambos grupos, lo que se interpreta como disminución del efecto terapéutico. Dos pacientes tuvieron reacciones adversas mínimas localizadas, las cuales se resolvieron con medidas generales. CONCLUSIÓN: El ácido hialurónico en ambas presentaciones, de alto y de bajo peso molecular, es efectivo en el tratamiento de la gonartrosis sintomática sin diferencia con el peso molecular.

Factores de riesgo psicosociales asociados a preeclampsia en mujeres mexicanas: análisis comparado en tres Estados / Psychosocial risk factors associated to pre-eclampsia among mexican women: a comparative analysis in three States / Fatores de risco psicossociais associados a pré-eclâmpsia em mulheres mexicanas: análise comparada em três Estados

Objetivo Analizar comparativamente los factores de riesgo psicosociales asociados con preeclampsia en mujeres mexicanas residentes de tres Estados. Método Estudio de casos y controles en 336 mujeres puérperas con y sin preeclampsia (130 del Estado de México, 114 de Yucatán y 92 de Veracruz). Se aplicaron dos cuestionarios y 5 escalas tipo Likert previamente validadas. Se llevó a cabo análisis estadístico comparativo mediante el software SPSS ver 20. Las variables cuantitativas se analizaron con Kruskal Wallis, las categóricas con frecuencias, el riesgo con razón de Momios (OR); IC de 95%. El riesgo se ajustó con regresión logística con a= 0.05. Resultados El análisis reveló violencia leve en los 3 Estados, con mayor frecuencia en el Estado de México y Veracruz (OR: 3.53; IC 95%: 1.13 - 10.97). La depresión, fue un hallazgo significativo para preeclampsia. Existe de 1 a 2 veces más riesgo para esta enfermedad en embarazadas del Estado de México que sufren algún grado de depresión (OR: 1.66; IC 95%: 1.058 - 2.607) y de 3 a 4 veces más riesgo cuando hay trastorno depresivo mayor (OR: 3.67; IC 95%: 1.23 - 10.89). En mujeres veracruzanas que reportaron algunas veces sintomatología depresiva está presente 3 veces más el riesgo de preeclampsia (OR: 3.12; IC 95%: 1.077 - 9.083) y 7.4 veces más riesgo cuando tienen menos de 5 consultas prenatales (OR: 7.42; IC 95%: 2.85 - 19.33). Conclusiones Se identificaron la depresión, sintomatología depresiva, violencia y el número de consultas prenatales menor a 5 como factores de riesgo psicosociales asociados a preeclampsia. Estos resultados permitirán proponer un modelo de intervención integral de enfermería para disminuirlos.

Objective To comparatively analyze the psychosocial risk factors associated to pre-eclampsia among Mexican women living in three States. Method This is a cases and controls study with 336 pregnant women with and without pre-eclampsia (130 from the State of Mexico, 114 from the state of Yucatan, and 92 from the state of Veracruz). Two questionnaires were administered, and 5 previously validated Likert type scales were used. A statistical comparative analysis was performed using SPSS v.20 software. Quantitative variables were analyzed with the Kruskal-Wallis procedure, categorical variables with frequencies calculation, and the associated risk with odds ratios in the 95% confidence interval. The associated risk was further adjusted through logistic regression a = 0.05). Results The analysis revealed a mild level of violence in all three States. States which reflected more frequency of violence risks were Mexico and Veracruz (OR: 3.53; IC 95%: 1.13 - 10.97). Depression was a significant finding associated to pre-eclampsia. A 1 to 2 times higher risk of associated pre-eclampsia was found among pregnant women with some degree of depression in the State of Mexico (OR: 1.66; IC 95%: 1.058 - 2.607), and this risk was 3 to 4 times higher in the presence of major depressive disorder (OR: 3.67; IC 95%: 1.23 - 10.89). Among women from the state of Veracruz who sometimes reported having depressive symptoms, the risk of pre-eclampsia association was 3 times higher (OR: 3.12; IC 95%: 1.077 - 9.083), while this risk was 7.4 times higher when these women had less than 5 prenatal assessment visits (OR: 7.42; IC 95%: 2.85 - 19.33). Conclusions Depression, depressive symptomatology, violence, and less-than-5 prenatal assessment visits were all identified as psychosocial risk factors associated to pre-eclampsia. These findings will support proposals for a corresponding nursing integral intervention model to address the related risk factors.

Objetivo Analisar comparativamente os fatores de risco psicossociais associados com pré-eclâmpsia em mulheres mexicanas residentes de três Estados. Método Estudo de casos e controles em 336 mulheres puérperas com e sem pré-eclâmpsia (130 do Estado do México, 114 de Yucatán e 92 de Veracruz). Aplicaram-se dois questionários e 5 escalas tipo Likert previamente validadas. Levou-se a cabo análise estatística comparativa mediante o software SPSS ver 20. As variáveis quantitativas analisaram-se com Kruskal Wallis, as categóricas com frequências, o risco com razão de Momios (OR); IC de 95%. O risco ajustou-se com regressão logística com a = 0.05. Resultados A análise revelou violência leve nos 3 Estados, com maior frequência no Estado de México e Veracruz (OR: 3.53; IC 95%: 1.13 -10.97). A depressão foi um achado significativo para pré-eclâmpsia. Existe de 1 a 2 vezes mais risco para esta doença em grávidas do Estado do México que sofrem algum grau de depressão (OR: 1.66; IC 95%: 1.058 - 2.607) e de 3 a 4 vezes mais risco quando tem transtorno depressivo maior (OR: 3.67; IC 95%: 1.23 - 10.89). Em mulheres veracruzanas que reportaram algumas vezes sintomatologia depressiva está presente 3 vezes mais o risco de pré-eclâmpsia (OR: 3.12; IC 95%: 1.077 -9.083) e 7.4 vezes mais risco quando têm menos de 5 consultas pré-natais (OR: 7.42; IC 95%: 2.85 - 19.33). Conclusões Identificaram-se a depressão, sintomatologia depressiva, violência e o número de consultas pré-natais menor a 5 como fatores de risco psicossociais associados à pré-eclâmpsia. Estes resultados permitiram propor um modelo de intervenção integral de enfermagem para diminuilos.

Molecular Epidemiology of Trypanosomatids and Trypanosoma cruzi in Primates from Peru.

We determined the prevalence rate and risk of infection of Trypanosoma cruzi and other trypanosomatids in Peruvian non-human primates (NHPs) in the wild (n = 126) and in different captive conditions (n = 183). Blood samples were collected on filter paper, FTA cards, or EDTA tubes and tested using a nested PCR protocol targeting the 24Sα rRNA gene. Main risk factors associated with trypanosomatid and T. cruzi infection were genus and the human-animal context (wild vs captive animals). Wild NHPs had higher prevalence of both trypanosomatids (64.3 vs 27.9%, P < 0.001) and T. cruzi (8.7 vs 3.3%, P = 0.057), compared to captive NHPs, suggesting that parasite transmission in NHPs occurs more actively in the sylvatic cycle. In terms of primate family, Pitheciidae had the highest trypanosomatid prevalence (20/22, 90.9%) and Cebidae had the highest T. cruzi prevalence (15/117, 12.8%). T. cruzi and trypanosomatids are common in Peruvian NHPs and could pose a health risk to human and animals that has not been properly studied.

Prevalence of Trypanosoma cruzi and Other Trypanosomatids in Frequently-Hunted Wild Mammals from the Peruvian Amazon.

To better understand the ecology of Trypanosoma cruzi in the northeastern Peruvian Amazon, we evaluated the prevalence of T. cruzi and other trypanosomatids in four orders of wild mammals hunted and consumed by inhabitants of three remote indigenous communities in the Peruvian Amazon. Of 300 wild mammals sampled, 115 (38.3%) were infected with trypanosomatids and 15 (5.0%) with T. cruzi. The prevalence of T. cruzi within each species was as follows: large rodents (Cuniculus paca, 5.5%; Dasyprocta spp., 2.6%), edentates (Dasypus novemcinctus, 4.2%), and carnivores with higher prevalence (Nasua nasua, 18.8%). The high prevalence of T. cruzi and other trypanosomatids in frequently hunted wild mammals suggests a sizeable T. cruzi sylvatic reservoir in remote Amazonian locations.

Síndrome de Vogt-Koyanagi-Harada en niños / Vogt-Koyanagi-Harada syndrome in children

Vogt-Koyanagi-Harada (VKH) syndrome is a systemic inflammatory disease that causes chronic and bilateral granulomatous panuveitis, usually described in adults. Objectives: To describe manifestations and complications of VKH in pediatric patients. Methods: Retrospectivedescriptive study upon patients <14 years-old with VKH, attended from January 1985 to July 2010 in three different centers. Results: A total of 17 patients (34 eyes) were studied; 9 (53%) female. The mean age was 10.8 years-old. Among extraocular manifestations; neurological (71%), dermatological (29%) and auditive (24%) signs were observed. Ocular findings included optic-disc involvement (94%), anterior uveitis (79%), choroiditis (77%), serous retinal detachment (71%) and vitritis (71%). Initial visual acuity (VA) was ≤0.05 in 47% of cases and ≥0.6 in 12% of patients. 71% presented complications: glaucoma (20 eyes), sinechiae (10 eyes), maculopathy (6 eyes) cataract (5 eyes) and ptisis bulbi (1 eyes). 35% received only corticosteroids and 65% inmunosupressive drugs. After treatment, 6% had VA ≤0.05 and 59% ≥0.6. Ten patients (59%) recurred: 30% compromising posterior pole, and 50% recurred >3 times. Conclusions: VKH in children is infrequent. It presents with optic-disc involvement and complications of posterior pole. It requires a high degree of suspicion, quick evaluation and early treatment, which include inmunosupressive and extended corticosteroid therapy. Nevertheless, a high rate of recurrence is seen among this group of patients. (AU)

Vida cotidiana y preeclampsia: Experiencias de mujeres del Estado de México / Daily life and pre-eclampsia: Experiences of women at the State of Mexico / Vida cotidiana e pré-eclâmpsia: Experiências de mulheres do Estado do México

Objetivo: Analizar la experiencia de vivir con preeclampsia en el contexto de la vida cotidiana de un grupo de mujeres del Estado de México que asisten al Hospital General de Chimalhuacán. Método: Investigación cualitativa de abordaje fenomenológico, hermenéutico con enfoque teórico de Alfred Schütz. La muestra fue definida por medio del criterio de saturación teórica. Los datos fueron recolectados a través de una entrevista semiestructurada y analizados con el método de van Manen. Seis criterios de rigor científico se utilizaron: Credibilidad, transferibilidad, consistencia, confirmabilidad, relevancia y adecuación teórico epistemológica. Resultados: Emergieron 5 categorías: 1) significado de la preeclampsia en la vida cotidiana; 2) experiencia de la dimensión física y emocional de vivir con preeclampsia; 3) interpretación del autocuidado en la vida cotidiana de la mujer con preeclampsia; 4) redes de apoyo que ayudan a sobrevivir la experiencia de tener preeclampsia; y 5) significado de los servicios de salud. Conclusiones: La preeclampsia es una enfermedad del embarazo de origen multifactorial y una de las principales causas de muerte materna en México, con impacto significativo en los servicios de salud. Se demuestra que en las mujeres con preeclampsia se potencializa su vulnerabilidad y riesgo de morir, al vivir en crisis, pues se ve trastocada y alterada su vida cotidiana, no solo en los aspectos biológicos al sentir malestares físicos y corporales, sino también en la parte emocional. Se observó la capacidad de auto-eco-socio-organización para equilibrar su entorno y vida cotidiana. Se ratificó que las redes de apoyo familiar y social son dadoras de bienestar e indispensables para fortalecer el cuidado.

Objective: To analyze the experience of living with pre-eclampsia among women at the State of Mexico who are attended at the General Hospital of Chimalhuacan. Method: This is a qualitative research with a phenomenological and hermeneutic approach with the theoretical focus of Alfred Schütz. The sample was defined using the criteria of theoretical saturation. Data were collected through a semi-structured interview and analyzed using the Van Manen method. Six scientific rigor criteria were used: credibility, transferability, consistency, confirmability, relevance, and theoretical and epistemological adequacy. Results: Five categories emerged: 1) meaning of pre-eclampsy in daily life, 2) physical and emotional experience of living with pre-eclampsy, 3) interpretation of self-care in the daily life of women with pre-eclampsy, 4) supporting networks which help women survive the experience of having pre-eclampsy, and 5) meaning of the Health Services. Conclusions: Pre-eclampsy, a multi-factorial pregnancy illness with a high impact on Health Services, is one of the main causes of maternal deaths in Mexico. Because of their living in constant crisis, women with pre-eclampsy have higher vulnerability and risk of death, and their lives are affected both physically and emotionally. Here, it was ratified that supporting networks are critical to address this problem and strengthen these women's capacity to achieve a balance in their environment and daily life.

Objetivo: Analisar a experiência de viver com pré-eclâmpsia no contexto da vida cotidiana de um grupo de mulheres do Estado do México que assistem ao Hospital General de Chimalhuacán. Método: Pesquisa qualitativa de abordagem fenomenológica, hermenêutica com enfoque teórico de Alfred Schütz. A amostra foi definida utilizando o critério de saturação teórica. Os dados foram coletados a través de uma entrevista semiestruturada e analisados com o método de Van Manen. Seis critérios de rigor científico utilizaram-se: credibilidade, transferibilidade, consistência, confirmação, relevância e adequação teórico epistemológica. Resultados: emergiram 5 categorias: 1) significado da pré-eclâmpsia na vida cotidiana, 2) experiência da dimensão física e emocional de viver com pré-eclâmpsia, 3) interpretação do autocuidado na vida cotidiana da mulher com pré-eclâmpsia, 4) redes de apoio que ajudam a sobreviver a experiência de ter pré-eclâmpsia, e, 5) significado dos serviços de saúde. Conclusões: a pré-eclâmpsia é uma doença de gravidez de origem multifatorial e uma das principais causas de morte materna no México com impacto significativo nos serviços de saúde. Mostra-se que nas mulheres com pré-eclâmpsia potencializa-se sua vulnerabilidade e risco de morrer, no viver em crise, pois vê-se modificada e alterada sua vida cotidiana, não só nos aspectos biológicos ao sentir mal-estares físicos e corporais, senão também na parte emocional. Observou-se a capacidade de auto-eco-sócio-organização para equilibrar o seu entorno e vida cotidiana. Ratificou-se que as redes de apoio familiar e social são dadoras de bem-estar e indispensáveis para fortalecer o cuidado.

Respiratory tract infection caused by Mycobacterium bovis in a black swan (Cygnus atratus).

A 3-year-old male black swan (Cygnus atratus), belonging to a private collection, died suddenly and was subjected to post mortem examination. At necropsy, caseous exudate was observed in the lungs and air sacs; granulomatous lesions characterized by epithelioid macrophages and abundant mycobacteria were observed microscopically. Avian tuberculosis associated with Mycobacterium bovis was confirmed by bacteriologic isolation, biochemical tests and molecular methods. The organism was identified as spoligotype SB0140, which is frequently found in cattle and people in North America. In this case, interspecies transmission could have been the source of infection because the swan cohabited with cattle.

Nivel de riesgo y aparición de úlceras por presión en pacientes en estado crítico / Risk level and pressure ulcer in patients in critical status / Nível de risco e aparição de úlceras por pressão em pacientes em estado crítico

La úlcera por presión es un evento constante en salud pública, un problema persistente en las unidades críticas y una preocupación de enfermería al verse involucrada en el cuidado que demanda el 50% más del tiempo utilizado. Objetivo: Describir el riesgo de aparición de las úlceras por presión en los pacientes ingresados en la unidad de cuidados críticos, con relación al tiempo de medición y estadio. Métodos: Estudio cuantitativo, observacional, descriptivo y longitudinal. Muestra: conformada por 31 pacientes. El instrumento fue aplicado en 4 momentos: Al ingreso, a las 24 y 72 h y al séptimo día de estancia. En cada medición se determinó el riesgo con la escala Braden y se valoró la piel. Resultado: El 100% de los pacientes tuvo alto riesgo al ingreso y 24 h; se redujo en un 71% a los 7 días. El 6.4% desarrolló úlcera por presión al ingreso, el 12.9% a las 24 h y el 35.5% a las 72 h. En general el 45.2% de los pacientes considerados como de alto riesgo manifestaron úlcera por presión a los 7 días de estancia hospitalaria. Se observó una correlación entre las variables nivel de riesgo y aparición de úlcera por presión a las 72 h (rs p = 0.04). Conclusión: A pesar de que se reduce el riesgo a la mitad en las primeras 72 h, aumenta la incidencia de úlcera por presión en ese mismo lapso; así, es importante que se realicen evaluaciones y revaloraciones desde el ingreso y durante los 7 primeros días de estancia hospitalaria en los pacientes críticamente enfermos utilizando un instrumento de valor predictivo.

Pressure ulcer are a constant event in public health, a persistent problem in units of critical care, and a nursing concern due to the longtime of care they need to be addressed. Objective To describe the risk of developing pressure ulcer which patients admitted into the critical care taking in consideration the time of measurement and stay. Methods This is a quantitative, observational, descriptive, and longitudinal study with a sample of 31 patients. The instrument was administered at four times: At entry, during the 24 and 72 hours, and at the seventh day of stay. In each measurement, the risk was assessed through the Braden scale. The skin status was also assessed. Result: 100% of patients were at high risk during the first 24 hours; and 71% of them were at high risk at the seventh day of admission. 6.4% developed a pressure ulcer during admission, 12.9% at 24 hours, and 35.5% at 72 hours. In general, 45.2% of the patients considered in high risk developed some pressure ulcer at the seventh day of stay. A correlation was observed among the variables risk level and pressure sores at 72 hours (rs P = .04>). Conclusion: Although the risk is reduced to the half during the first 72 hours, the incidence of pressure ulcer increases in the same period; thus, it is important to perform assessments and re-appraisements to the critically ill patients using a predictive instrument since hospital admission and during the first seven days of stay.

A úlcera por pressão é um evento constante em saúde pública, um problema persistente nas unidades críticas e uma preocupação de enfermagem ao ser envolvida no cuidado que demanda 50% mais do tempo utilizado. Objetivo: Descrever o risco de aparição das úlceras por pressão nos pacientes internados na unidade de cuidados críticos, em relação ao tempo de medição e estádio. Métodos: Estudo quantitativo, observacional, descritivo e longitudinal. Amostra conformada por 31 pacientes. O instrumento foi aplicado em quatro momentos: à entrada, às 24 e 72 horas e ao sétimo dia da permanência. Em cada medição determinou-se o risco com a escala Braden e avaliou-se a pele. Resultado: 100% dos pacientes teve alto risco à entrada e 24 horas; diminuiu em um 71% aos sete dias. 6.4% desenvolveu úlcera por pressão à entrada, 12.9% às 24 horas e 35.5% às 72 horas. Em geral 45.2% dos pacientes considerados como de alto risco manifestaram úlcera por pressão aos sete dias de permanência hospitalar. Observou-se uma correlação entre as variáveis nível de risco e aparição de úlcera por pressão às 72 horas (rs = 0.04>). Conclusão: Apesar de que se reduz o risco à metade nas primeiras 72 horas, aumenta a incidência de úlcera por pressão no mesmo período, assim, é importante que se realizem avaliações e reavaliações desde a entrada e durante os sete primeiros dias de permanência nos pacientes criticamente doentes, utilizando um instrumento de valor preditivo.

Factores sociodemográficos y hospitalarios relacionados con el nivel de ansiedad en familiares con pacientes pediátricos / Social, demographic, and hospital factors related to anxiety levels among relatives of pediatric patients / Fatores sociodemográficos e hospitalares relacionados com o nível de ansiedade em familiares com pacientes pediátricos

Introducción: La asistencia al niño hospitalizado y su familia es uno de los ámbitos que hoy requiere un esfuerzo por investigar. Conocer los factores relacionados con la ansiedad que viven los familiares en la UCIP, abre caminos para desarrollar modelos de intervención y Planes de Cuidados de Enfermería dirigidos a favorecer el afrontamiento y mejorar el bienestar familiar intrahospitalario. Objetivo: Determinar la relación de los factores sociodemográficos y hospitalarios con el nivel de ansiedad que presentan los familiares con pacientes hospitalizados en la unidad de cuidados intensivos pediátricos (UCIP). Metodología: Estudio cuantitativo, descriptivo y transversal. Se utilizó un instrumento de elaboración propia para la identificación de factores sociodemográficos y hospitalarios; y la escala de Inventario de Ansiedad Estado-Rasgo de Spielberger (IDARE) para la medición del nivel de ansiedad. Resultados: 34 familiares que participaron -11.8% (4) hombres; y 88.2% (30) mujeres- la edad promedio fue de 29 años, las puntuaciones de ansiedad estado y ansiedad rasgo indican que al no conocer algún albergue se presenta una diferencia significativa en el nivel de ansiedad estado de los familiares (X

Introduction: Aid to hospitalized children and their relatives is an issue which requires further research. Knowing the factors related to the anxiety that these children's relatives experience in the PIUC can broaden the scope to develop intervention models and Nursing Care Plans to address these issues and improve the intra-hospital family wellbeing. Objective: To determine the relationship between the social, demographic, and hospital factors with the levels of anxiety experienced by the relatives of hospitalized patients in the Pediatric Intensive Care Unit (PICU). Methodology: Quantitative, descriptive, and transversal study. An own-designed instrument to identify social, demographic, and hospital factors, and the Spielberger's State-Trait Anxiety Inventory (STAI) to estimate the levels of anxiety were used. Results: Thirty four relatives participated, 11.8% (4) were male and 88.2% (30) female. The average age was 29 years old. The anxiety state and trait scores suggest that, when information on shelters in not available, there are significant differences in the state of anxiety (X

Introduçãoa: assistência da criança hospitalizada e da sua família, é um dos âmbitos que na atualidade requer um esforço para pesquisar. Conhecer os fatores relacionados com a ansiedade que experimentam os familiares na UCIP, abre caminhos para desenvolver modelos de intervenção e Planos de Cuidados de Enfermagem dirigidos a favorecer o enfrentamento e melhorar o bem-estar familiar intra-hospitalar. Objetivo: Determinar a relação dos fatores sociodemográficos e hospitalares com o nível de ansiedade que apresentam os familiares com pacientes hospitalizados na Unidade de Cuidados Intensivos Pediátricos (UCIP). Metodologia: Estudo Quantitativo, descritivo e transversal. Utilizou-se um instrumento de elaboração própria para a identificação de fatores sociodemográficos e hospitalares; e a escala de Inventário de Ansiedade Estado-Traço de Spielberger (IDARE) para a medição do nível de ansiedade. Resultados: 34 familiares participaram, 11.8% (4) homens e 88.2% (30) mulheres, a média da idade foi de 29 anos, as pontuações de ansiedade estado e ansiedade traço, indicam que quando conhecer algum abrigo se apresenta uma diferença significativa no nível de ansiedade estado dos familiares (X

Validación psicométrica de escalas PSS-14, AFA-R, HDRS, CES-D, EV en puérperas mexicanas con y sin preeclampsia / Psychometric validation of scales PSS-14, AFA-R, HDRS, CES-D, EV in postpartum Mexican women with and without preeclampsia / Validação das escalas PSS, AFA-R, HDRS, CES-D e EV em puérperas mexicanas com e sem pré-eclâmpsia

Objetivo: Validar las escalas psicométricas: Estrés percibido (PSS), Apoyo familiar y de amigos (AFA-R), Depresión de Hamilton (HDRS), Sintomatología depresiva (CES-D), Violencia e índice de severidad (EV) en mujeres mexicanas puérperas con y sin preeclamsia. Método: Estudio descriptivo que valida las propiedades psicométricas de las escalas PSS, AFA-R, HDRS, CES-D y EV. Las escalas fueron seleccionadas a través de una búsqueda en la web de los últimos 5 años. Las escalas fueron aplicadas por enfermeras mediante entrevista a 104 puérperas hospitalizadas con y sin preeclampsia. Para la confiabilidad se aplicó el alfa de Cronbach. La validez fue confirmada por un grupo de expertos y prueba piloto. Se utilizó análisis factorial por los métodos componentes principales, Káiser y Varimax. Resultados: Cada escala tuvo consistencia interna; calificaron con nivel aceptable (PSS 0.718 y EV 0.740) y nivel bueno (AFA-R 0.911, CES-D 0.869 y HDRS 0.806). La validez de contenido fue aprobada con: PSS 96.42%; EV 100%; AFA 100%; CES-D 98.75%, y HDRS 92.64%. La estructura factorial estuvo bien distribuida: cada factor obtuvo algunos pesos altos y los demás, próximos a cero. Cada variable estuvo saturada en uno o en 2 factores, por lo que casi no compartieron varianzas. PSS, con 6 factores, tuvo poder explicativo de la varianza total del 72.23%; AFA-R, con 3 factores y poder explicativo del 74.19%; HDRS, con 6 factores y poder explicativo del 70.58%; CES-D, con 6 factores y poder explicativo del 72.87%, y la escala EV tuvo varianza de cero. Conclusiones: Los hallazgos señalan que las escalas PSS, AFA-R, HDRS y CES-D por su confiabilidad, validez y utilidad funcionan teóricamente bien para medir las variables en el grupo de mujeres estudiadas; sin embargo para la escala EV debe considerarse un análisis factorial diferente en otra muestra similar.

Objective: To assess the following scales' psychometric characteristics: Perceived Stress (PSS), Family and Friends Support (AFA-R), Depression-Hamilton (HDRS), Depressive Symptomatology (CES-D), Violence and Index of Severity (EV) among Mexican populations, with and without pre-eclampsia. Method: Descriptive and psychometric study. A web search was conducted to decide on which scales use. Nurses applied the scales to 104 hospitalized post-partum women with and without pre-eclampsia. Cronbach alpha was measured to assess reliability. Validity was confirmed by a group of experts and by a pilot study. Principal components, Kaiser, and varimax factor analyses were carried out.ResultsEach scale had internal consistency-acceptable level: PSS .718 and EV .740; good level: AFA-R .911, CES-D .869 and HDRS .806. Content validity was assessed as PSS: 96.42%, EV: 100%, AFA 100%, CES-D: 98.75% and HDRS: 92.64%. The factor structure was well distributed. Each factor had few important weights, and the rest were close to zero. Each variable was not saturated except in one, and barely in two, factors, thus variance was almost not shared. PSS, with 6 factors, had an explicative power of the total variance of 72.23%; AFA-R, with 3 factors and explicative power of 74.19%; HDRS with 6 factors and explicative power of 70.58%; while CES-D, with 6 factors too, had an explicative power of 72.87%. The EV scale had a variance of zero. Conclusions: Findings suggest that the scales PSS, AFA-R, HDRS, and CES-D, due to their reliability, validity, and usefulness, theoretically work well to assess the different variables among the group of studied women. Another factor analysis on the EV scale should be considered for a similar sample.

Objetivo: Validar as escalas psicométricas: Estrese Percebido (PSS), Apoio Familiar e de Amigos (AFA-R), Depressão de Hamilton (HDRS), Sintomatologia Depressiva (CES-D), Violência e Índice DE Severidade (EV) em mulheres mexicanas puérperas com e sem pré-eclâmpsia. Método: Estudo descritivo que valida as propriedades psicométricas das escalas PSS, AFA-R, HDRS, CES-D e EV. As escalas foram escolhidas a través de uma busca na web dos últimos 5 anos. As escalas foram aplicadas por enfermeiras mediante entrevista a 104 puérperas hospitalizadas com e sem pré-eclâmpsia. Para a confiabilidade aplicou-se alfa de Crombach. A validade foi confirmada por um grupo de experientes e prova piloto. Utilizou-se analise fatorial pelos métodos componentes principais, Káiser e Varimax. Resultados: Cada escala teve consistência interna; qualificaram com nível aceitável (PSS 0.718 y EV 0.740) e com nível bom (AFA-R 0.911, CES-D 0.869 y HDRS 0.806). A validade de conteúdo foi aprovada com: PSS 96.42%; EV 100%; AFA 100%; CES-D 98.75%, e HDRS 92.64%. A estrutura fatorial esteve bem distribuída, cada fator obteve alguns pesos altos e os outros próximos a zero. Cada variável esteve saturada em um ou em outros fatores, pelo que quase não compartilharam variâncias. PSS, com 6 fatores, teve poder explicativo da variância total do 72.23%; AFA-R, com 3 fatores e poder explicativo do 74.19%; HDRS, com 6 fatores e poder explicativo do 70.58%; CES-D, com 6 fatores e poder explicativo do 72.87%, e a escala EV teve variância de zero. Conclusões: As descobertas assinalam que as escalas PSS, AFA-R, HDRS e CES-D por sua confiabilidade, validade e utilidade funcionam teoricamente bem para medir as variáveis no grupo de mulheres estudadas; porém, para a escala EV deve se considerar uma análise fatorial diferente em outra amostra semelhante.

Perinatal factors associated with the development of cow's milk protein allergy.

UNLABELLED: The prevalence of cow's milk protein allergy (CMPA) has increased in recent years, and is associated with antimicrobial use during the perinatal period, prematurity, the type of childbirth, and the decrease in breastfeeding. The aim of this study was to analyze whether there is any association between these factors and the development of CMPA. MATERIAL AND METHODS: A retrospective, comparative, cross-sectional, observational study was conducted by reviewing the case records of 101 children diagnosed with CMPA and seen at the Department of Gastroenterology and Nutrition of the Instituto Nacional de Pediatría within the time frame of January 2012 and August 2013. The following variables were included: age, sex, weeks of gestation, history of maternal infection and antimicrobial use during the pregnancy, type of delivery, and feeding with human milk, and its duration. Likewise, the case records of 90 children were reviewed as a control group on not having CMPA or any other allergy. The chi-square test was used for proportions, and the Mann-Whitney U test was used for comparing means in the statistical analysis. RESULTS: The factors associated with CMPA were the use of antimicrobials during gestation and breastfeeding duration in months. Both factors were statistically significant (P<.001). No association was found between CMPA and gestational age or type of delivery. CONCLUSIONS: The statistically significant associated factors were breastfeeding duration and the use of antimicrobials during the gestational stage. These results underline the necessity for prospective studies.

Effects of repeated electroejaculations on kinematic sperm subpopulations and quality markers of Mexican creole goats.

Here we show the effects of repeated electroejaculation (EE) on mean values of motility, mitochondrial functionality, and expression of active caspases on goat sperm obtained by EE. Evaluations were done using CASA and flow cytometry. A strategy for identification of kinematic sperm subpopulations, when individual data of sperm are not provided by the CASA system, is provided. Fifty semen samples, five of each of ten adult creole goats, were obtained by electroejaculation. Mean values of total motility, progressive motility and flow cytometry evaluations were compared among EEs. Relationships among mean values of variables were investigated using Spearman correlation and principal component analysis (PCA). For identification of kinematic sperm subpopulations, PCA followed by hierarchical clustering was applied on data of the intervals provided automatically by the CASA system. Total motility does no change after repeated EE. Mean values of motility parameters and molecular markers were unrelated in multivariate space, but bivariate correlations were found. Values in upper and lower intervals defined clearly the sperm subpopulations, which had motility parameters changing over time. Taken together, our results show that repeated EE does not affect mean values of total motility, that molecular markers are not related with motility parameters, and that it is possible to identify kinematic sperm subpopulations when individual data, of motility parameters, are not provided by the CASA system.

Porocarcinoma ecrino: reporte de un caso / Eccrine porocarcinoma: a case report

Los tumores anexiales de la piel representan un grupo heterogéneo de entidades de baja frecuencia de presentación. Su origen es controvertido, quizás a partir de células madres pluripotenciales, aceptándose principalmente dos líneas embriológicas: pilosebáceo-apócrina y ecrina. Se los clasifica en cuatro grupos principales: tumores del folículo piloso, tumores sebáceos, tumores apócrinos y tumores ecrinos. Su distribución anatómica refleja áreas con mayor densidad de anexos cutáneos, presentándose en forma única o múltiple.

Skin adnexal tumors are represented as a heterogeneous group of entities of low frequency range. Its origin is controversial, perhaps from pluripotent stem cells, mainly from two embryological lines: pilosebaceous-apocrine and eccrine. They are classified as four main groups: tumors of the hair follicle, sebaceous tumors, apocrine tumors and eccrine tumors. Their anatomic distribution reflects areas with the highest density of cutaneous adnexal, arising in either single or multiple forms.

Salmonella enterica serovar Typhimurium BaeSR two-component system positively regulates sodA in response to ciprofloxacin.

In response to antibiotics, bacteria activate regulatory systems that control the expression of genes that participate in detoxifying these compounds, like multidrug efflux systems. We previously demonstrated that the BaeSR two-component system from Salmonella enterica serovar Typhimurium (S. Typhimurium) participates in the detection of ciprofloxacin, a bactericidal antibiotic, and in the positive regulation of mdtA, an efflux pump implicated in antibiotic resistance. In the present work, we provide further evidence for a role of the S. Typhimurium BaeSR two-component system in response to ciprofloxacin treatment and show that it regulates sodA expression. We demonstrate that, in the absence of BaeSR, the transcript levels of sodA and the activity of its gene product are lower. Using electrophoretic mobility shift assays and transcriptional fusions, we demonstrate that BaeR regulates sodA by a direct interaction with the promoter region.