Polish adaptation and validation of the Short Inventory of Problems (SIP-2L). / Polska adaptacja i walidacja Krótkiej skali problemów (SIP-2L).

OBJECTIVES: The aim of the research was to present the Polish adaptation and validation of the Short Inventory of Problems (SIP-2L) by Miller et al. (1995). The SIP-2L is a popular selfreport measure used to assess the negative consequences of alcohol use in 5 domains with negative consequences: physical, interpersonal, intrapersonal, social and impulse control. The adapted version of SIP assesses lifetime consequences of using alcohol. METHODS: A total of 256 participants diagnosed with alcohol dependence (71 women and 185 men) completed an assessment battery that included SIP-2L and Polish versions of AUDIT, MAST and KOEP-R, which is a modified Polish adaptation of CEAQ - Comprehensive Effects of Alcohol Questionnaire. RESULTS: Confirmatory factor analysis demonstrated the hypothesized 5-factor model produced the best fit. Reliability of the entire method (Cronbach's α = 0.868) and for all factors was satisfactory, as was convergent validity. CONCLUSIONS: The results support that the Polish version of the SIP-2L has good psychometric properties and can successfully assess the consequences of drinking among patients diagnosed with alcohol dependence.

Interactions of lamotrigine with single- and double-stranded DNA under physiological conditions.

This study presents evaluation of the possible mechanisms of interaction between the antiepileptic drug lamotrigine (LMT) and single- and double-stranded DNA (ssDNA and dsDNA, respectively). These interactions were studied in phosphate-buffered saline (PBS) at physiological pH 7.4 by cyclic voltammetry (CV) and square wave voltammetry (SWV) using a glassy carbon electrode (GCE) in a bulk incubated solution. The addition of both types of DNA to LMT solution decreased peak currents and led to a negative shift in peak potentials, thus indicating the dominance of electrostatic interactions. UV-Vis absorption spectroscopy was also used to assess the interaction between ds/ssDNA and LMT. The data obtained from spectroscopic analysis confirmed that electrostatic interaction is the predominant interaction between LMT and both types of DNA. The calculated binding constants for LMT-dsDNA and LMT-ssDNA complexes as determined by SWV were 6.46 × 105 and 1.81 × 106, respectively, while the values obtained from UV-Vis spectroscopy were 6.93 × 105 and 1.19 × 106, respectively. The obtained results indicated a higher affinity of LMT for ssDNA than for dsDNA.

Comparative Electroanalytical Studies of Graphite Flake and Multilayer Graphene Paste Electrodes.

In this paper, the fabrication, surface characterisation and electrochemical properties of graphite flake (GFPE) and multilayer graphene (MLGPE) paste electrodes are described. The Raman investigations and scanning electron microscopy were used to analyze and compare structure of both carbon materials. The electroanalytical performance of both electrodes was examined and compared on the basis of the square-wave and cyclic voltammetric behavior of acetaminophen and model redox systems. Results of those studies revealed that GFPE has a larger electroactive surface area and better conductive properties, whilst MLGPE demonstrate better analytical characteristic in case of acetaminophen (AC) determination. AC determination was developed using square wave voltammetry (SWV) and square wave stripping voltammetry (SWSV). For both working electrodes, the process of accumulation enabled us to obtain an extended linear range and to lower the detection limit. In pharmaceutical formulations, AC was determined with good recovery.

Rapid electroanalytical procedure for sesamol determination in real samples.

In this study, the development of an electroanalytical assay based on square wave voltammetry technique for determining sesamol (Ses) in sesame oil samples is described. The influence of various factors such as pH of the supporting electrolyte, its composition, and SW (square wave) parameters was studied. Linearity of the peak current depended on the concentration of Ses in the range from 3.0 to 140.0 µmol L-1 with a limit of detection of 0.71 µmol L-1. Furthermore, the cyclic voltammetric behavior of Ses and the effects of scan rate and pH on the peak current and peak potential of Ses were determined. Moreover, the electrode process was found to be diffusion-controlled. The proposed methodology was successfully applied for determining Ses in commercial sesame oil samples. The obtained results were in good agreement with the results from the HPLC-UV reference method.

Electrochemical and spectroscopic studies of the interaction of antiviral drug Tenofovir with single and double stranded DNA.

In the present study, the electrochemical behavior of the antiviral drug tenofovir (tnf) on a boron-doped diamond electrode (BDDE) has been studied using square-wave voltammetry (SWV). The experimental conditions such as the supporting electrolyte composition and the SWV parameters were optimized. Under the optimized conditions, a simple and sensitive SWV procedure for tnf determination was developed in the concentration range of 5.0 × 10-6 to 1.0 × 10-4 mol L-1. The calculated limit of detection and limit of quantification were equal to 5.6 × 10-7 and 1.9 × 10-6 mol L-1, respectively. The biological significance of the developed method was demonstrated by a quantitative analysis of the pharmaceutical formulations Viread and Tenofovir disoproxil Teva. Moreover, both voltammetric and spectroscopic techniques were used to study the interaction between tnf and DNA. Changes in the electrochemical and spectroscopic behavior of tnf in the presence of DNA were used to calculate the binding constants of the formed complexes. The estimated values of Gibbs free energy revealed that the formation of the drug-DNA complexes was a spontaneous and favorable process. Moreover, for free and bound tenofovir, kinetic parameters such as heterogeneous rate constant, electron transfer coefficient, and diffusion coefficient were determined.