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1.
Rev Sci Tech ; 39(3): 907-922, 2020 Jan.
Article in English | MEDLINE | ID: mdl-35275127

ABSTRACT

The Pantanal and Cerrado biomes in the state of Mato Grosso contain migratory bird sites in the municipalities of Cáceres and Araguaiana, respectively. The levels of avian influenza (AI) and Newcastle disease (ND) viral activity in backyard poultry at these sites are unknown owing to a lack of studies. Considering the risk of introduction of AI and ND to Brazil from migratory birds, as well as the importance of active surveillance in the detection and prevention of diseases for official control, monitoring in these poultry populations is faster, more practical and cheaper for official service veterinarians. The objective of this study was to verify the presence of AI and ND viral activity in backyard poultry reared near these migratory bird sites in the years 2016 and 2019. Serum samples and cloacal and tracheal swab samples collected from chickens, turkeys, quails, ducks and geese were evaluated by indirect diagnostic methods including enzyme-linked immunosorbent assay and haemagglutination inhibition tests and direct detection of viral sequences using quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR). No positive samples were detected by qRT-PCR.The frequencies of birds seropositive for AI and ND were 0.7% and 19.6% in 2016 and 0.5% and 17.2% in 2019, respectively, in Araguaiana and 0.8% and 32.3% in 2016 and 7.0% and 38.1% in 2019, respectively, in Cáceres. Antibodies belonging to AI subtypes H1, H4, H6 and H14 were identified in Cáceres in 2019. Spatial analysis showed an agglomeration of farms with seropositive poultry within the urban area of Cáceres, suggesting AI and ND virus activity in this area. This study showed no circulation of the notifiable AI subtypes H5 and H7 or the ND virus in backyard poultry raised around migratory bird sites in the state of Mato Grosso. The results of the present study support evidence indicating that the circulation of strains with low pathogenicity in urban areas enables backyard poultry to serve as a source of infection for other birds; thus, increased surveillance is necessary in this population.


Les biomes du Pantanal et du Cerrado dans l'état du Mato Grosso contiennent deux sites accueillant des oiseaux migrateurs, situés respectivement dans les comtés de Cáceres et d'Araguaiana. Faute d'études de terrain, le niveau d'activité virale de l'influenza aviaire et de la maladie de Newcastle chez les volailles de basse-cour de ces deux sites était jusqu'à présent inconnu. Compte tenu du risque d'introduction au Brésil de l'influenza aviaire et de la maladie de Newcastle par les oiseaux migrateurs, et de l'importance de la surveillance active pour détecter et prévenir ces maladies dans le cadre des activités de lutte conduites par les Services vétérinaires, il est plus pratique, moins onéreux et plus rapide pour les vétérinaires des services officiels d'axer la surveillance sur les populations de volailles de basse-cour. Les auteurs présentent les résultats d'une étude conduite en 2016 et en 2019 pour déterminer le niveau de circulation des virus de l'influenza aviaire et de la maladie de Newcastle chez les volailles de basse-cour élevées à proximité des sites d'oiseaux migrateurs. Des échantillons sériques et des écouvillons cloacaux et trachéaux prélevés sur des poulets, des dindes, des cailles, des canards et des oies ont été soumis à des méthodes de diagnostic indirectes telles que les épreuves immuno-enzymatique et d'inhibition de l'hémagglutination, et à une méthode de détection directe des séquences virales par amplification en chaîne par polymérase quantitative en temps réel couplée à une transcription inverse (qRT-PCR). Aucun échantillon positif n'a été détecté par qRT-PCR. Les taux de séropositivité respectivement à l'influenza aviaire et à la maladie de Newcastle étaient, chez les volailles prélevées à Araguaiana, de 0,7 % et 19,6 % en 2016, et de 0,5 % et 17,2 % en 2019 ; chez les volailles prélevées à Cáceres, ils étaient de 0,8 % et 32,3 % en 2016, et de 7,0 % et 38,1 % en 2019. Les anticorps détectés à Cáceres en 2019 appartenaient aux sous-types H1, H4, H6 et H14 du virus de l'influenza aviaire. L'analyse spatiale a révélé une concentration importante d'élevages ayant des volailles séropositives dans la zone urbaine de Cáceres, indiquant une activité des virus de l'influenza aviaire et de la maladie de Newcastle dans cette région. Aucune circulation des sous-types H5 et H7 à déclaration obligatoire du virus de l'influenza aviaire ni du virus de la maladie de Newcastle n'a été mise en évidence chez les volailles de basse-cour élevées autour des sites d'oiseaux migrateurs dans l'état du Mato Grosso. Les résultats de cette étude étayent les données d'après lesquelles les volailles de basse-cour des zones urbaines deviennent des sources d'infection pour d'autres espèces d'oiseaux à la faveur d'une circulation de souches faiblement pathogènes ; il est donc nécessaire de renforcer la surveillance dans cette population.


Los biomas de Pantanal y Cerrado, situados en el estado de Mato Grosso, albergan espacios frecuentados por aves migratorias en los municipios de Cáceres y Araguaiana, respectivamente. Debido a la falta de estudios al respecto, se desconocen los niveles de actividad de los virus de la influenza aviar (IA) y de la enfermedad de Newcastle (EN) en las aves de corral caseras de estas zonas. Teniendo en cuenta el riesgo de introducción en el Brasil de la IA y la EN por conducto de aves migratorias, así como la importancia de una vigilancia activa para la detección y prevención de enfermedades con fines de control oficial, para los veterinarios de los servicios públicos resulta más rápido, práctico y barato vigilar esas poblaciones de aves de corral. Los autores describen un estudio encaminado a comprobar, en los años 2016 y 2019, la actividad de los virus de la IA y la EN en bandadas caseras de aves de corral criadas cerca de los antedichos espacios de aves migratorias. Tras obtener muestras séricas e hisopados cloacales y traqueales de pollos, pavos, codornices, patos y gansos, se analizaron las muestras con técnicas de diagnóstico indirecto (ensayo inmunoenzimático y prueba de inhibición de la hemaglutinación) y de detección directa de secuencias víricas (retrotranscripción acoplada a reacción en cadena de la polimerasa cuantitativa en tiempo real: qRT-PCR). No se detectó ninguna muestra positiva por qRT-PCR. En cuanto a las tasas de seropositividad para la IA y la EN, en Araguaiana resultaron positivas el 0,7% y el 19,6%, respectivamente, de las aves analizadas en 2016, por un 0,5% y un 17,2% en 2019, mientras que en Cáceres lo fueron el 0,8% y el 32,3% en 2016 y el 7,0% y el 38,1% en 2019. En 2019 se identificaron en Cáceres anticuerpos correspondientes a los subtipos H1, H4, H6 y H14 del virus de la influenza aviar. El análisis espacial puso de relieve una aglomeración de fincas con aves de corral seropositivas en la zona urbana de Cáceres, hecho indicativo de que en la zona hay actividad de los virus de la IA y la EN. El estudio no evidenció circulación alguna de los subtipos H5 y H7 del virus de la IA, que son de declaración obligatoria, ni del virus de la EN en las aves de corral caseras criadas en los alrededores de los espacios del estado de Mato Grosso que albergan aves migratorias. Los resultados del estudio parecen avalar los datos que indican que la circulación de cepas poco patógenas en zonas urbanas hace de las bandadas caseras una posible fuente de infección para otras aves, razón por cual es tanto más necesario redoblar la vigilancia de estas poblaciones de aves de corral.

2.
Parasite Immunol ; 39(7)2017 Jul.
Article in English | MEDLINE | ID: mdl-28397969

ABSTRACT

The inflammasome is a multiprotein signalling platform involved in the pathogenesis of various inflammatory skin diseases. Herein, we investigated gene and protein expression of the inflammasome molecules AIM2 and NLRP3 in active lesions from patients with L. (V.) braziliensis-associated tegumentary leishmaniasis (TL) and correlated these findings with the clinical presentations and responses to therapy. Real-time PCR assays showed a significantly higher AIM2 gene expression in mucosal leishmaniasis (ML) compared with that in cutaneous leishmaniasis (CL). Additionally, AIM2 mRNA expression was significantly higher in lesions from poor responders than in lesions from good responders. In situ protein quantification analyses revealed greater AIM2 expression in ML lesions than in CL lesions. The percentage of AIM2-producing cells was higher in poor responders than in good responders. Although not quite significant, IL-1ß+ cells were slightly more prominent in poor responders than in good responders. Similar results were observed when patients were evaluated according to clinical form. GP63 immunostaining was identified in all samples, but no significant variation between mucosal and cutaneous lesions was observed. GP63 could be associated with reduced NLRP3 inflammasome expression in CL and ML patients. Taken together, these data demonstrate that AIM2 is an important component of the inflammasome in TL patients and is directly associated with the severity of lesions.


Subject(s)
DNA-Binding Proteins/metabolism , Inflammasomes , Leishmania braziliensis/immunology , Leishmaniasis, Cutaneous/immunology , Leishmaniasis, Mucocutaneous/immunology , Adult , Animals , DNA-Binding Proteins/genetics , Female , Glucosamine/analogs & derivatives , Glucosamine/therapeutic use , Humans , Interleukin-1beta/metabolism , Leishmaniasis, Cutaneous/drug therapy , Leishmaniasis, Cutaneous/parasitology , Leishmaniasis, Mucocutaneous/drug therapy , Leishmaniasis, Mucocutaneous/parasitology , Male , Metalloendopeptidases/genetics , Metalloendopeptidases/metabolism , Middle Aged , NLR Family, Pyrin Domain-Containing 3 Protein/genetics , NLR Family, Pyrin Domain-Containing 3 Protein/metabolism , Real-Time Polymerase Chain Reaction
3.
Vaccine ; 30(41): 5973-7, 2012 Sep 07.
Article in English | MEDLINE | ID: mdl-22828589

ABSTRACT

BACKGROUND: Antibody responses to standard regimens of hepatitis B (HBV) vaccination are lower in HIV-infected subjects and the best hepatitis B vaccine schedule in this population is not known. OBJECTIVE: To assess the immunogenicity and to evaluate predictors of serologic response of a modified regimen of a HBV recombinant vaccine in a cohort of HIV-infected subjects. METHODS: HIV-infected subjects received 4 doses (40 µg) of a recombinant HBV vaccine at 0, 1, 2 and 6 months. Demographic information as well as CD4 cell count and plasma viral load were assessed at baseline. Protective and strong responses were defined as an anti-HBs titer ≥10 mIU/mL and ≥100 mIU/mL, respectively and were evaluated one month after the third and the fourth doses. RESULTS: 163 HIV-infected individuals were evaluated 67 (40%) were male and median age was 37 years. Median CD4 cell count was 385 cells/mm(3) and 113 (70%) had undetectable HIV-1 viral load. Protective antibody response was observed in 83 and 91% and a strong antibody response was observed in 62 and 80% of the subjects after 3 and 4 doses, respectively. In a multivariate logistic model undetectable HIV-1 viral load and higher CD4 cell counts were independent predictors of a strong antibody response after 4 doses. Patients with undetectable HIV viral load were almost 3 times more likely to have anti-HBs titers above 100 mIU/mL than those with detectable viral load. CONCLUSIONS: A 4-double-dose regimen of a recombinant HBV vaccine increased response rates and determined higher antibody titers which may translate in prolonged protection against HBV. Inclusion of a fourth dose of HBV vaccine for HIV-infected subjects should be considered in the public health setting.


Subject(s)
HIV Infections/physiopathology , Hepatitis B Antibodies/blood , Hepatitis B Vaccines/administration & dosage , Hepatitis B/prevention & control , Vaccination/methods , Adult , Antibody Formation/immunology , Antiretroviral Therapy, Highly Active , CD4 Lymphocyte Count , Dose-Response Relationship, Immunologic , Female , HIV Infections/immunology , HIV Infections/virology , HIV-1/immunology , HIV-1/pathogenicity , Hepatitis B/immunology , Hepatitis B/virology , Hepatitis B Antibodies/immunology , Humans , Immunization Schedule , Male , Vaccines, Synthetic/administration & dosage , Viral Load
4.
Vaccine ; 28(6): 1447-50, 2010 Feb 10.
Article in English | MEDLINE | ID: mdl-19995540

ABSTRACT

We evaluated a modified HBV regimen in a cohort of HIV-infected subjects in Rio de Janeiro, Brazil. HIV-infected subjects with no serologic evidences of previous hepatitis B infection were immunized with 4 doses (40 microg each) of recombinant hepatitis B vaccine given at 0, 1, 2 and 6 months. Blood samples were collected 1 month after the last dose and anti-HBs titers were measured. A protective antibody response was defined as an anti-HBs titer >or=10 mIU/mL. Forty-seven subjects (30 women, 17 men; mean age was 36 years, ranging from 21 to 58 years) were included in the final analysis. Median baseline CD4+ lymphocyte count was 402 cells/mm(3) and 33 subjects (70%) had an HIV viral load below 80 copies/mL. A protective antibody response was observed in 42 (89%) subjects. Thirty-seven (78%) and 28 (60%) patients developed anti-HBs titers higher than 100 mIU/mL and 1000 mIU/mL, respectively. 1 out of 5 non-responders (20%) had an HIV viral load below the detection limit, in contrast with 32 (76%) of those with an adequate serologic response (p=0.02). These findings suggest that 4-double dose alternative schedule may be considered to overcome the lower seroconversion rates observed with the standard regimens in HIV-infected subjects.


Subject(s)
HIV Infections/immunology , Hepatitis B Antibodies/blood , Hepatitis B Vaccines/administration & dosage , Hepatitis B Vaccines/immunology , Hepatitis B/prevention & control , Immunization Schedule , Vaccination/methods , Adult , Brazil , CD4 Lymphocyte Count , Female , Humans , Immunization, Secondary/methods , Male , Middle Aged , Viral Load , Young Adult
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