ABSTRACT
The clinical involvement and antifungal susceptibility of Aspergillus section Circumdati are poorly known. We analyzed 52 isolates, including 48 clinical isolates, belonging to 9 species inside the section Circumdati. The whole section exhibited, by the EUCAST reference method, a poor susceptibility to amphotericin B, but species/series-specific patterns were observed for azole drugs. This underlines the interest in getting an accurate identification inside the section Circumdati to guide the choice of antifungal treatment in clinical practice.
Subject(s)
Antifungal Agents , Aspergillus , Antifungal Agents/pharmacology , Microbial Sensitivity Tests , Amphotericin B/pharmacology , Azoles/pharmacologyABSTRACT
To evaluate the in vitro susceptibility of Fusarium to isavuconazole, 75 clinical isolates were identified using matrix-assisted laser desorption ionization-time of flight mass spectrometry and then tested with a broth microdilution method (EUCAST) and the gradient concentration strip (GCS) technique. The activity of isavuconazole overall was shown to be limited, with an MIC50 of >16 µg/ml, without significant differences between the species complexes. The categorical agreement between GCS and EUCAST was 97.4% to 100%, making the GCS as a valuable alternative.
Subject(s)
Antifungal Agents/pharmacology , Fusarium/drug effects , Nitriles/pharmacology , Pyridines/pharmacology , Triazoles/pharmacology , Fusariosis/microbiology , Microbial Sensitivity TestsSubject(s)
Tinea/transmission , Trichophyton , Zoonoses/transmission , Adult , Animals , Antifungal Agents/administration & dosage , Ciclopirox/administration & dosage , Disease Reservoirs , Female , Humans , Imidazoles/administration & dosage , Ointments , Rats, Hairless , Tinea/drug therapy , Tinea/veterinary , Zoonoses/drug therapyABSTRACT
Water-borne transmission of the coccidium Cryptosporidium parvum Tyzzer, 1912 is frequently responsible for outbreaks of human cryptosporidiosis. One of the most important was reported in 1993 in Milwaukee in the United States, where 403,000 cases were recorded. The determination of the percentage of oocysts excystated is the first step in evaluating their viability, but it alone is not sufficient. This percentage depended on the conditions of storage and also the presence of oxidant or disinfectent agents in water. The percentage of excystation is not always related to viability. Therefore, determination of the viability of excysted sporozoites by determining their infectivity for enterocytic Caco2 cell lines in culture provides information essential for evaluating the risk of contaminated drinking water.
