ABSTRACT
Bone marrow transplant recipients are highly susceptible to opportunistic fungal infections. This is the report, of the first case of a Chaetomium systemic infection described in Brazil. A 34 year-old patient with chronic myeloid leukemia underwent an allogeneic sibling matched bone marrow transplant. Seven months later, he developed systemic infection with enlargement of the axillary and cervical lymph nodes. Culture of the aspirates from both lymph nodes yielded Chaetomium globosum. The infection was successfully treated with amphotericin B. The increasing population of immunosupressed patients requires a careful microbiologic investigation for uncommon fungal infections.
Subject(s)
Bone Marrow Transplantation/adverse effects , Chaetomium/isolation & purification , Mycoses/immunology , Adult , Amphotericin B/therapeutic use , Antifungal Agents/therapeutic use , Fatal Outcome , Humans , Immunocompromised Host , Leukemia, Myelogenous, Chronic, BCR-ABL Positive/surgery , Lymph Nodes/microbiology , Male , Mycoses/drug therapy , Mycoses/microbiologyABSTRACT
BACKGROUND: Pichia anomala is a rare cause of fungemia. From February to April, 1998, eight cases of fungemia occurred in the intensive care and high risk units of the Nursery. There were four infants with P. anomala infection, one of whom also had Candida parapsilosis infection, two cases with C. parapsilosis infection and two with Candida albicans infection. OBJECTIVE: To determine factors associated with fungemia in the intensive care and high risk units of the Nursery, especially P. anomala. METHODS: A cohort study with 59 newborns. RESULTS: Factors associated with fungemia were: central venous catheter (CVC) (P = 0.0006); total parenteral nutrition (TPN) (P = 0.0005); lipid emulsion (P = 0.002); previous antimicrobial use (P = 0.002); and other invasive procedures (P = 0.002). Factors associated with P. anomala fungemia were: CVC (P = 0.004); TPN (P = 0.018); previous antibiotic use (P = 0.037); and other invasive procedures (P = 0.037). Evaluation of the units demonstrated that there were several technical problems involving administration of TPN that was manipulated in the Nursery without precautions. Changes in TPN formulation and education as to adequate technique were implemented. During follow-up (1998 to 1999) only two fungemias occurred that were caused by C. albicans. Cultures of hands of personnel were negative for P. anomala. Electrophoretic karyotyping of P. anomala showed three profiles. CONCLUSIONS: Factors associated with fungemia were catheter use, invasive procedures and total parenteral nutrition, suggesting that the acquisition of P. anomala was exogenous.
Subject(s)
Cross Infection/epidemiology , Disease Outbreaks , Fungemia/epidemiology , Intensive Care, Neonatal , Pichia/isolation & purification , Analysis of Variance , Brazil/epidemiology , Cohort Studies , Cross Infection/diagnosis , Female , Fungemia/diagnosis , Humans , Incidence , Infant, Newborn , Male , Parenteral Nutrition/adverse effects , Probability , Risk Assessment , Risk Factors , Urinary Catheterization/adverse effectsABSTRACT
Trichosporon species are emerging as opportunistic agents that cause systemic diseases in immunocompromised patients. Patients undergoing bone marrow transplant are submitted to intense and prolonged periods of neutropenia and consequently to several risk factors to fungal infections as the use of broad spectrum antibiotics and invasive devices. Two cases of fungal infections caused by Trichosporon asahii var. asahii and T. inkin in patients with bone marrow transplant are described T. asahii var. asahii was responsible for fungemia and the identification of this microorganism was later performed. T. inkin caused vascular accesses infection and was recovered from an implanted Hickman-Broviac catheter. Both patients were under oral fluconazole prophylaxis. The patient with systemic infection died despite the therapy with amphotericin B and the patient with catheter-related infection recovered from the fungal infection after catheter removal. Difficulties in the identification of this microorganism lead to delays in treatment and post-mortem diagnosis.
Subject(s)
Bone Marrow Transplantation , Fungemia/diagnosis , Mycoses/diagnosis , Postoperative Complications/microbiology , Trichosporon , Adult , DNA, Fungal/analysis , DNA, Ribosomal/analysis , Fatal Outcome , Female , Fungemia/drug therapy , Humans , Immunocompromised Host , Leukemia, Myelogenous, Chronic, BCR-ABL Positive/surgery , Leukemia, Myeloid, Acute/surgery , Male , Mycoses/drug therapy , Polymerase Chain Reaction , Postoperative Complications/drug therapyABSTRACT
One hundred clinical isolates of Cryptococcus neoformans from human immunodeficiency virus (HIV)-infected and non-HIV-infected patients from Brazil, Chile, and Venezuela were separated according to varieties and tested for antifungal susceptibility. A high susceptibility to antifungal agents was observed among all the isolates. The electrophoretic karyotyping of 51 strains revealed good discrimination among Cryptococcus neoformans var. neoformans strains.
