ABSTRACT
Edwardsiella tarda is a bacterium that is associated with both an asymptomatic oral carrier state and self-limiting diarrhoeal illness in humans. We herein report a case of sepsis and liver abscess caused by E. tarda and review the patients with E. tarda sepsis who presented at our hospital. An 85-year-old woman developed enterocolitis and sepsis caused by E. tarda. She was administered intensive care and thus was able to soon show a good recovery, however, she subsequently developed a liver abscess. During a complicated course, she continued to be treated with antibiotics and after a successful course was discharged on the 44th day after admission.
Subject(s)
Edwardsiella tarda , Enterobacteriaceae Infections/drug therapy , Liver Abscess/drug therapy , Sepsis/drug therapy , Aged , Aged, 80 and over , Anti-Bacterial Agents/therapeutic use , Diabetes Complications/drug therapy , Edwardsiella tarda/isolation & purification , Edwardsiella tarda/pathogenicity , Enterobacteriaceae Infections/complications , Enterocolitis/drug therapy , Enterocolitis/etiology , Female , Humans , Liver Abscess/etiology , Sepsis/complicationsABSTRACT
Exercise-induced collapse (EIC) is an autosomal recessive disorder in Labrador retrievers. In this study, an allele-specific PCR was developed to detect the point mutation G767T in exon 6 of canine DNM1, previously shown to be responsible for canine EIC. Of 133 Labrador retrievers tested in Japan, 6 (4.5%) were homozygous (EIC) and 50 (37.6%) were heterozygous (carriers) for the G767T mutation.
Subject(s)
Dog Diseases/genetics , Dynamin I/genetics , Muscle Weakness/veterinary , Physical Conditioning, Animal/adverse effects , Polymerase Chain Reaction/methods , Polymorphism, Genetic , Animals , Dog Diseases/physiopathology , Dogs , Genotyping Techniques/veterinary , Japan , Muscle Weakness/genetics , Muscle Weakness/physiopathology , Pedigree , Point Mutation , Polymerase Chain Reaction/veterinary , Polymorphism, Restriction Fragment LengthABSTRACT
Pairs of the ECF sigma factor and its anti-sigma factor, SigW and RsiW, of Bacillus-related species that inhabit extreme environments were heterologously expressed in B. subtilis. All the RsiWs, membrane proteins, failed to fill their function of repressing cognate SigW activity, despite their close structural similarities. Particularly, uncontrolled expression of Oceanobacillus iheyensis OISigW due to abortive OIRsiW was harmful to B. subtilis. Analysis of revertants of this growth defect and site-directed mutagenesis indicated that the insertion of six and a minimum of three hydrophobic amino acid residues occurring in the transmembrane region allowed OIRsiW to function as anti-OISigW. Subcellular localization of OIRsiW was detected by immunoblot analysis, suggesting that both the wild-type and the mutant form of OIRsiW were localized to the membrane. An appropriate length of a transmembrane region required for proper integration into the membrane after translocation might vary among these Bacillus-related species.
