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1.
Metabolites ; 14(4)2024 Apr 21.
Article in English | MEDLINE | ID: mdl-38668371

ABSTRACT

Sugarcane (Saccharum spp. hybrids) and its processed products have supported local industries such as those in the Nansei Islands, Japan. To improve the sugarcane quality and productivity, breeders select better clones by evaluating agronomic characteristics, such as commercially recoverable sugar and cane yield. However, other constituents in sugarcane remain largely unutilized in sugarcane breeding programs. This study aims to establish a data-driven approach to analyze agronomic characteristics from breeding programs. This approach also determines a correlation between agronomic characteristics and free amino acid composition to make breeding programs more efficient. Sugarcane was sampled in clones in the later stage of breeding selection and cultivars from experimental fields on Tanegashima Island. Principal component analysis and hierarchical cluster analysis using agronomic characteristics revealed the diversity and variability of each sample, and the data-driven approach classified cultivars and clones into three groups based on yield type. A comparison of free amino acid constituents between these groups revealed significant differences in amino acids such as asparagine and glutamine. This approach dealing with a large volume of data on agronomic characteristics will be useful for assessing the characteristics of potential clones under selection and accelerating breeding programs.

2.
Microorganisms ; 10(3)2022 Mar 06.
Article in English | MEDLINE | ID: mdl-35336149

ABSTRACT

Virulence factors, such as staphylococcal enterotoxin A (SEA), are contained within membrane vesicles (MVs) in the cell membrane of Staphylococcus aureus. In this study, the effects of the growth stage on quantitative and qualitative changes in the components contained in the MVs of S. aureus SEA-producing strains were examined. Changes in the expression levels of S. aureus genes were examined at each growth stage; phenol-soluble modulin (PSM) gene reached a maximum after 8 h, and the expression of cell membrane-related genes was decreased after 6 h. Based on these gene expression patterns, MVs were prepared at 6, 17, and 24 h. The particle size of MVs did not change depending on the growth stage. MVs prepared after culture for 17 h maintained their particle size when stored at 23 °C. The amount of SEA in the culture supernatant and MVs were not correlated. Bifunctional autolysin, a protein involved in cell wall biosynthesis/degradation, was increased in MVs at 17 h. The expression pattern of inflammation-related genes in human adult low calcium high temperature (HaCaT) cells induced by MVs was different for each growth stage. The inclusion components of S. aureus-derived MVs are selective, depend on the stage of growth, and may play an important role in toxicity.

3.
Biosci Biotechnol Biochem ; 85(10): 2191-2194, 2021 Sep 22.
Article in English | MEDLINE | ID: mdl-34279595

ABSTRACT

1'-Acetoxychavicol acetate (ACA) is found in Thai ginger (Alpinia galanga) and is a powerful agonist of transient receptor potential ankyrin 1 (TRPA1). In a diet-induced obesity mouse model, ACA reduced fat deposition. Sympathetic nerve activation was also indicated in the ACA-fed group. This study is expected to promote the utilization of food containing TRPA1 agonists to treat obesity.


Subject(s)
Benzyl Alcohols , Animals , Ankyrins , Zingiber officinale , Intra-Abdominal Fat , Mice
4.
Biosci Biotechnol Biochem ; 85(5): 1175-1182, 2021 Apr 24.
Article in English | MEDLINE | ID: mdl-33686392

ABSTRACT

Ergothioneine (ERGO), a thiohistidine betaine, exists in various fungi, plants, and animals. Humans take in ERGO from their diet. ERGO is a strong biological antioxidant, but there are only a limited number of reports about its redox mechanism. The purpose of this study was to clarify the oxidation mechanism of ERGO. Reactions of ERGO with chemical oxidants were performed. The oxidation products of ERGO were analyzed by nuclear magnetic resonance and liquid chromatography-mass spectrometry (LC-MS). The major product of oxidation of ERGO by hydrogen peroxide in physiological conditions was identified as hercynine (histidine betaine). One molecule of ERGO was able to reduce 2 molecules of hydrogen peroxide. Hercynine was found to react with the more potent oxidant hypochlorite. One unstable decomposition product was detected by LC-MS. As a result, a mechanism of oxidation of ERGO, and hence its physiological antioxidant activity, was developed.


Subject(s)
Antioxidants/chemistry , Betaine/analogs & derivatives , Calcium Compounds/chemistry , Ergothioneine/chemistry , Histidine/analogs & derivatives , Hydrogen Peroxide/chemistry , Oxidants/chemistry , Betaine/chemistry , Chromatography, Liquid , Histidine/chemistry , Kinetics , Oxidation-Reduction , Solutions , Tandem Mass Spectrometry , Water/chemistry
5.
Theor Appl Genet ; 128(10): 2037-46, 2015 Oct.
Article in English | MEDLINE | ID: mdl-26152572

ABSTRACT

KEY MESSAGE: Genetic analysis and gene mapping of the 4-methylthio-3-butenyl glucosinolate-less trait of white radish were performed and a white radish cultivar with new glucosinolate composition was developed. A spontaneous mutant having significantly low 4-methylthio-3-butenyl glucosinolate (4MTB-GSL) content was identified from a landrace of Japanese white radish (Raphanus sativus L.) through intensive evaluation of glucosinolate profiles of 632 lines including genetic resources and commercial cultivars using high-performance liquid chromatography (HPLC) analysis. A line lacking 4MTB-GSL was developed using the selected mutant as a gene source. Genetic analyses of F1, F2, and BC1F1 populations of this line suggested that the 4MTB-GSL-less trait is controlled by a single recessive allele. Using SNP and SCAR markers, 96 F2 plants were genotyped, and a linkage map having nine linkage groups with a total map distance of 808.3 cM was constructed. A gene responsible for the 4MTB-GSL-less trait was mapped between CL1753 and CL5895 at the end of linkage group 1. The genetic distance between these markers was 4.2 cM. By selfing and selection of plants lacking 4MTB-GSL, a new cultivar, 'Daikon parental line No. 5', was successfully developed. This cultivar was characterized by glucoerucin, which accounted for more than 90% of the total glucosinolates (GSLs). The total GSL content in roots was ca. 12 µmol/g DW, significantly lower than those in common white radish cultivars. Significance of this line in radish breeding is discussed.


Subject(s)
Glucosinolates/chemistry , Raphanus/chemistry , Raphanus/genetics , Alleles , Chromatography, High Pressure Liquid , Chromosome Mapping , Genes, Plant , Genes, Recessive , Genetic Linkage , Genotype , Plant Breeding , Polymorphism, Single Nucleotide
6.
Breed Sci ; 64(1): 48-59, 2014 May.
Article in English | MEDLINE | ID: mdl-24987290

ABSTRACT

Unique secondary metabolites, glucosinolates (S-glucopyranosyl thiohydroximates), are naturally occurring S-linked glucosides found mainly in Brassicaceae plants. They are enzymatically hydrolyzed to produce sulfate ions, D-glucose, and characteristic degradation products such as isothiocyanates. The functions of glucosinolates in the plants remain unclear, but isothiocyanates possessing a pungent or irritating taste and odor might be associated with plant defense from microbes. Isothiocyanates have been studied extensively in experimental in vitro and in vivo carcinogenesis models for their cancer chemopreventive properties. The beneficial isothiocyanates, glucosinolates that are functional for supporting human health, have received attention from many scientists studying plant breeding, plant physiology, plant genetics, and food functionality. This review presents a summary of recent topics related with glucosinolates in the Brassica family, along with a summary of the chemicals, metabolism, and genes of glucosinolates in Brassicaceae. The bioavailabilities of isothiocyanates from certain functional glucosinolates and the importance of breeding will be described with emphasis on glucosinolates.

7.
J Nat Med ; 64(2): 133-8, 2010 Apr.
Article in English | MEDLINE | ID: mdl-20140532

ABSTRACT

Species identification of five Dendrobium plants was conducted using phylogenetic analysis and the validity of the method was verified. Some Dendrobium plants (Orchidaceae) have been used as herbal medicines but the difficulty in identifying their botanical origin by traditional methods prevented their full modern utilization. Based on the emerging field of molecular systematics as a powerful classification tool, a phylogenetic analysis was conducted using sequences of two plastid genes, the maturase-coding gene (matK) and the large subunit of ribulose 1,5-bisphosphate carboxylase-coding gene (rbcL), as DNA barcodes for species identification of Dendrobium plants. We investigated five medicinal Dendrobium species, Dendrobium fimbriatum, D. moniliforme, D. nobile, D. pulchellum, and D. tosaense. The phylogenetic trees constructed from matK data successfully distinguished each species from each other. On the other hand, rbcL, as a single-locus barcode, offered less species discriminating power than matK, possibly due to its being present with little variation. When results using matK sequences of D. officinale that was deposited in the DNA database were combined, D. officinale and D. tosaense showed a close genetic relationship, which brought us closer to resolving the question of their taxonomic identity. Identification of the plant source as well as the uniformity of the chemical components is critical for the quality control of herbal medicines and it is important that the processed materials be validated. The methods presented here could be applied to the analysis of processed Dendrobium plants and be a promising tool for the identification of botanical origins of crude drugs.


Subject(s)
DNA, Plant/genetics , Dendrobium/genetics , Drugs, Chinese Herbal , Endoribonucleases/genetics , Nucleotidyltransferases/genetics , Phylogeny , Ribulose-Bisphosphate Carboxylase/genetics , Drugs, Chinese Herbal/isolation & purification , Plant Leaves/genetics , Plant Stems/genetics , Sequence Analysis, DNA/methods
8.
Life Sci ; 85(1-2): 60-9, 2009 Jul 03.
Article in English | MEDLINE | ID: mdl-19409911

ABSTRACT

AIMS: Most of the terpenoids with an alpha,beta-unsaturated 1,4-dialdehyde moiety, which are found in plants, fungi, and insects, have a pungent taste. However, the neural receptors responsible for the pungency of these terpenoids have not been identified yet. The transient receptor potential ankyrin 1 (TRPA1) and transient receptor potential vanilloid 1 (TRPV1), which are expressed in the nociceptive neurons, induce a sensation of heat on activation by some pungent ingredients in food. In this study, we selected miogadial (MD), miogatrial (MT), and polygodial (PG) from the terpenoids with an alpha,beta-unsaturated 1,4-dialdehyde moiety and examined the effects of these 3 terpenoids on TRPA1 or TRPV1. MAIN METHODS: TRPV1 and TRPA1 activity by 3 terpenoids were evaluated using Ca(2+) imaging and patch-clamp methods in mammalian cells that express TRP heterologously and mouse sensory neurons. KEY FINDINGS: The 3 terpenoids activated TRPA1 that was heterologously expressed in HEK293 or CHO cells. The potencies of activation by the 3 terpenoids were equal and almost 10 times stronger than that of allyl isothiocyanate (AITC), which is known as the most potent TRPA1 agonist among all natural products. Moreover, these 3 terpenoids exhibited increased intracellular Ca(2+) concentration in mouse sensory neuron cells compared to AITC. High concentrations of the 3 terpenoids also activated TRPV1 that was heterologously expressed in HEK293 cells. SIGNIFICANCE: These results indicated that MD, MT, and PG were more potent in activating TRPA1 than TRPV1, and suggested that they primarily activate TRPA1 to induce pungency.


Subject(s)
Aldehydes/chemistry , Diterpenes/chemistry , Diterpenes/pharmacology , Transient Receptor Potential Channels/agonists , Animals , Behavior, Animal/drug effects , CHO Cells , Calcium/metabolism , Cell Line , Cricetinae , Cricetulus , Electrophysiology , Ganglia, Spinal/cytology , Ganglia, Spinal/drug effects , Humans , Male , Mice , Mice, Inbred C57BL , Pain/psychology , Sensory Receptor Cells/drug effects , Sesquiterpenes/pharmacology , Structure-Activity Relationship , TRPA1 Cation Channel , TRPV Cation Channels/agonists
9.
Biosci Biotechnol Biochem ; 73(3): 641-7, 2009 Mar 23.
Article in English | MEDLINE | ID: mdl-19270388

ABSTRACT

Vietnamese coriander (Persicaria odorata Lour.) belongs to a group known as cilantro mimics with the 'cilantro' flavor, in which C10 and C12 aldehydes and alcohols have been found as the potent odor compounds. Their composition isolated by different extraction methods varied. The enzyme activity was assayed, and the reductase acting on some aliphatic aldehydes with NADH/NADPH as a coenzyme was found in a crude enzymatic system of fresh leaves. The maximum activity was observed at pH 8.0 in Na-phosphate and at pH 8.5 to 9.0 in a glycine-NaOH buffer, using heptanal as a substrate. The activated reductase that caused the alcohol generation to increase after a time was inhibited by p-hydroxymercuribenzoate. Our results, which are the first to be reported on Vietnamese coriander leaves, reveal the presence of aliphatic aldehyde dehydrogenase, which is responsible for acid formation, and elucidate the strong activity of the aliphatic aldehyde reductase.


Subject(s)
Alcohols/chemistry , Alcohols/metabolism , Aldehyde Reductase/metabolism , Plant Leaves/metabolism , Polygonaceae/metabolism , Aldehyde Reductase/antagonists & inhibitors , Aldehydes/chemistry , Aldehydes/metabolism , Enzyme Inhibitors/pharmacology , Hydrogen-Ion Concentration , Odorants/analysis , Plant Leaves/enzymology , Polygonaceae/enzymology , Time Factors , Vietnam , Volatilization
10.
Biosci Biotechnol Biochem ; 72(10): 2681-6, 2008 Oct.
Article in English | MEDLINE | ID: mdl-18838802

ABSTRACT

We isolated a novel pungent principle from the flower buds of myoga. Based on an interpretation of the HR-MS, EI-MS, IR, UV, and NMR data, this compound was (8betaH)-14,17-cyclolabda-12,14(17)-diene-15,16-dial (mioganal, 1). We determined the threshold value of mioganal to be 5.6x10(-5) g/ml, which is about eight times greater than that of 8beta(17)-epoxy-12(E)labdene-15,16-dial (miogadial), the main pungent principle in the flower buds of myoga. In addition, we examined the content of mioganal in different parts of different varieties. Mioganal is mainly distributed in the leaves of both natsumyoga and akimyoga varieties at 1.60 mg/100 g fresh weight on average, its amount being three times higher than that in the flower buds.


Subject(s)
Diterpenes/chemistry , Diterpenes/pharmacology , Taste Threshold/drug effects , Zingiberaceae/chemistry , Capsaicin/pharmacology , Humans , Magnetic Resonance Spectroscopy , Molecular Structure , Sesquiterpenes/pharmacology , Young Adult
11.
J Neurochem ; 107(5): 1248-60, 2008 Dec.
Article in English | MEDLINE | ID: mdl-18796006

ABSTRACT

Neurotrophins, such as the nerve growth factor (NGF), play an essential role in the growth, development, survival and functional maintenance of neurons in the central and peripheral systems. They also prevent neuronal cell death under various stressful conditions, such as ischemia and neurodegenerative disorders. NGF induces cell differentiation and neurite outgrowth by binding with and activating the NGF receptor tyrosine kinase followed by activation of a variety of signaling cascades. We have investigated the NGF-dependent neuritogenesis enhancer potential of a food-derived small molecule contained in Brassica vegetables and identified the protein tyrosine phosphatase (PTP) 1B as a key regulator of the NGF receptor-initiated signal transduction. Based on an extensive screening of Brassica vegetable extracts for the neuritogenic-promoting activity in the rat pheochromocytoma cell line PC12, we found the Japanese horseradish, wasabi (Wasabia japonica, syn. Eutrema wasabi), as the richest source and identified 6-methylsulfinylhexyl isothiocyanate (6-HITC), an analogue of sulforaphane isolated from broccoli, as one of the major neuritogenic enhancers in the wasabi. 6-HITC strongly enhanced the neurite outgrowth and neurofilament expression elicited by a low-concentration of NGF that alone was insufficient to induce neuronal differentiation. 6-HITC also facilitated the sustained-phosphorylation of the extracellular signal-regulated kinase and the autophosphorylation of the NGF receptor TrkA. It was found that PTP1B act as a phosphatase capable of dephosphorylating Tyr-490 of TrkA and was inactivated by 6-HITC in a redox-dependent manner. The identification of PTP1B as a regulator of NGF signaling may provide new clues about the chemoprotective potential of food components, such as isothiocyanates.


Subject(s)
Nerve Growth Factor/physiology , Protein Tyrosine Phosphatase, Non-Receptor Type 1/metabolism , Receptor, trkA/physiology , Signal Transduction/physiology , Animals , Biotinylation/methods , Cysteine/metabolism , Dose-Response Relationship, Drug , Enzyme Inhibitors/pharmacology , Flow Cytometry , Green Fluorescent Proteins/biosynthesis , Green Fluorescent Proteins/genetics , Hydrogen Peroxide/pharmacology , Isothiocyanates/pharmacology , Mitogen-Activated Protein Kinase 3/metabolism , Nerve Growth Factor/chemistry , Nerve Growth Factor/pharmacology , Neurites/drug effects , Neurites/metabolism , Oxidants/pharmacology , PC12 Cells , Phosphorylation/drug effects , RNA, Small Interfering/genetics , Rats , Receptor, trkA/biosynthesis , Receptor, trkA/genetics , Signal Transduction/drug effects , Transfection
12.
Biochem Biophys Res Commun ; 373(3): 429-34, 2008 Aug 29.
Article in English | MEDLINE | ID: mdl-18577375

ABSTRACT

In this study, we demonstrated that the two ginger-derived components have a potent and unique pharmacological function in 3T3-L1 adipocytes via different mechanisms. Both pretreatment of 6-shogaol (6S) and 6-gingerol (6G) significantly inhibited the tumor necrosis factor-alpha (TNF-alpha) mediated downregulation of the adiponectin expression in 3T3-L1 adipocytes. Our study demonstrate that (1) 6S functions as a PPARgamma agonist with its inhibitory mechanism due to the PPARgamma transactivation, and (2) 6G is not a PPARgamma agonist, but it is an effective inhibitor of TNF-alpha induced c-Jun-NH(2)-terminal kinase signaling activation and thus, its inhibitory mechanism is due to this inhibitory effect.


Subject(s)
Adipocytes/drug effects , Adiponectin/biosynthesis , Catechols/pharmacology , Fatty Alcohols/pharmacology , Tumor Necrosis Factor-alpha/antagonists & inhibitors , 3T3-L1 Cells , Adipocytes/metabolism , Animals , Down-Regulation/drug effects , Gene Expression/drug effects , Zingiber officinale/chemistry , JNK Mitogen-Activated Protein Kinases/antagonists & inhibitors , Mice , PPAR gamma/agonists , Tumor Necrosis Factor-alpha/pharmacology
13.
J Agric Food Chem ; 56(2): 512-6, 2008 Jan 23.
Article in English | MEDLINE | ID: mdl-18163555

ABSTRACT

The flavor-enhancing effects of the volatile constituents in celery were investigated. The test samples were prepared by adding celery fractions to chicken broth at a concentration that distinct odors of them were not detected, and the samples were sensorially evaluated for the perceived intensities of 8 terms such as "thick," "impactful," "mild," "lasting," "satisfied," "complex," "refined," and "clarified," which are considered to be the elements of the complex flavor and for 3 terms such as "sweet," "salty," and "umami" taste. A comparison of effects between the volatile and nonvolatile fractions of celery revealed that the volatile compounds in celery enhanced the complex flavor of chicken broth more than the nonvolatile compounds. Among the characteristic odorants of celery, three phthalides, namely, sedanenolide, 3- n-butylphthalide, and sedanolide, were shown to contribute to the complex flavor of chicken broth, and sedanenolide was most effective. The three phthalides enhanced perceived intensities of "umami" and "sweet" despite their no taste properties in addition to the complex flavor.


Subject(s)
Apium/chemistry , Chickens , Hot Temperature , Meat , Taste , Animals , Benzofurans/analysis , Humans , Odorants/analysis , Solutions , Volatilization
14.
Biosci Biotechnol Biochem ; 71(8): 1911-9, 2007 Aug.
Article in English | MEDLINE | ID: mdl-17690486

ABSTRACT

Phenolic glucosides having radical scavenging activity were examined from the fraction eluted with 20% methanol on Amberlite XAD-2 resin applied to lemon (Citrus limon) juice by using reversed phase chromatography. Four phenolic glucosides were identified as 1-feruloyl-beta-D-glucopyranoside, 1-sinapoyl-beta-D-glucopyranoside, 6,8-di-C-glucosylapigenin and 6,8-di-C-glucosyldiosmetin by (1)H-NMR, (13)C-NMR, and MS analyses. They exhibited radical scavenging activity for 1,1-diphenyl-2-picrylhydrazyl (DPPH) and superoxide, although the activity was low in comparison with eriocitrin, a potent antioxidant in lemon fruit, and the eriodictyol of its aglycone. The phenolic compounds in lemon juice were examined for their suppressive effect on the expression of blood adhesion molecules by measuring the expression of intercellular adhesion molecule-1 (ICAM-1) in human umbilical vein endothelial cells (HUVECs) induced by necrosis factor-alpha (TNF-alpha). 6,8-Di-C-glucosylapigenin, apigenin, and diosmentin of the flavones were found to significantly suppress the expression of ICAM-1 at 10 muM (P<0.05). The phenolic glucosides isolated in this study were contained in comparative abundance in daidai (Citrus aurantium) and niihime (Citrus unshiu x Citrus tachibana) among the sour citrus juices.


Subject(s)
Antioxidants/pharmacology , Cell Adhesion Molecules/genetics , Citrus/chemistry , Endothelial Cells/metabolism , Gene Expression Regulation/drug effects , Glucosides/pharmacology , Antioxidants/isolation & purification , Cells, Cultured , Endothelial Cells/cytology , Endothelial Cells/drug effects , Free Radical Scavengers/isolation & purification , Glucosides/isolation & purification , Humans , Magnetic Resonance Spectroscopy , Mass Spectrometry , Phenols/isolation & purification , Phenols/pharmacology , Phytotherapy , Umbilical Veins/cytology
15.
Biosci Biotechnol Biochem ; 71(2): 407-13, 2007 Feb.
Article in English | MEDLINE | ID: mdl-17284850

ABSTRACT

The mechanism of the formation of 4-hydroxy-2(or 5)-ethyl-5-(or 2)-methyl-3(2H)-furanone (HEMF) with yeast under caltivation in a medium containing amino-carbonyl reactants of ribose and glycine was investigated using stable isotopes of the corresponding compounds. It was confirmed that the skeleton of the five-membered ring and the methyl group of the side chain of HEMF was formed from ribose, and that the ethyl group was derived from the glucose metabolite by yeast. The formation of HEMF was confirmed when acetaldehyde as the glucose metabolite and a cell-free extract from yeast were added to the medium containing amino-carbonyl reactants. These results suggest that the role of yeast in HEMF formation is not only to provide the glucose metabolite, but also in combining the amino-carbonyl reactants with the glucose metabolite.


Subject(s)
Furans/metabolism , Soy Foods/analysis , Zygosaccharomyces/metabolism , Acetaldehyde/metabolism , Cell-Free System , Culture Media , Furans/chemistry , Gas Chromatography-Mass Spectrometry , Glucose/metabolism , Glycine/metabolism , Indicators and Reagents , Ribose/metabolism , Zygosaccharomyces/growth & development
16.
Nutr Neurosci ; 9(3-4): 169-78, 2006.
Article in English | MEDLINE | ID: mdl-17176640

ABSTRACT

We investigated the components of ginger that are involved in increasing body temperature. Gingerols ([6,8,10]-gingerols) and shogaols ([6,8,10]-shogaols) having different alkyl carbon chain lengths were targeted. All the gingerols and shogaols increased intracellular calcium concentration in rat transient receptor potential vanilloid subtype 1 (TRPV1)-expressing HEK293 cells via TRPV1. In this regard, the shogaols were more potent than the gingerols. Aversive responses were induced by [6]-, [10]-gingerol, and [6]-shogaol (5 mmol/l) in rats when these compounds were applied to the eye; however, no response was observed in response to [10]-shogaol (5 and 10 mmol/l). [10]-Shogaol induced nociceptive responses via TRPV1 in rats following its subcutaneous injection into the hindpaw; the pungent compound capsaicin (CAP) and [6]-shogaol were observed to have similar effects. Moreover, adrenal catecholamine secretion, which influences energy consumption, was promoted in rats in response to [6]- and [10]-gingerols and [6]- and [10]-shogaols (1.6 micromol/kg, i.v.). [10]-Shogaol-induced adrenaline secretion was inhibited by administration of capsazepine, a TRPV1 antagonist. In conclusion, gingerols and shogaols activated TRPV1 and increased adrenaline secretion. Interestingly, [10]-shogaol is the only nonpungent compound among the gingerols and shogaols, suggesting its usefulness as a functional ingredient in food.


Subject(s)
Calcium Channels/physiology , Catechols/pharmacology , Epinephrine/metabolism , Plant Extracts/pharmacology , TRPV Cation Channels/physiology , Zingiber officinale , Animals , Calcium/metabolism , Calcium Channels/drug effects , Capsaicin/pharmacology , Cell Line , Cooking , Humans , Kidney , Kinetics , Male , Rats , Rats, Sprague-Dawley , TRPV Cation Channels/drug effects
17.
Biosci Biotechnol Biochem ; 70(10): 2494-500, 2006 Oct.
Article in English | MEDLINE | ID: mdl-17031030

ABSTRACT

We screened myoga extracts for inhibitors of human platelet aggregation and human 5-lipoxygenase. We identified a novel labdane type of diterpene, together with three known diterpenes (miogadial and galanals A and B) from the flower buds of myoga. Spectroscopic data indicated the structure of the new compound to be 12(E)-labdene-15,16,(8beta)17-trial (miogatrial). Miogatrial and miogadial were potent inhibitors of human platelet aggregation and human 5-lipoxygenase (5-LOX). The sesquiterpene, polygodial, also exhibited strong inhibitory activity against human platelet aggregation and 5-LOX. On the other hand, galanals A and B did not have inhibitory activity in either experimental system. It thus appears that a 3-formyl-3-butenal structure was essential for the potent inhibition of human platelet aggregation and human 5-LOX.


Subject(s)
Aldehydes/pharmacology , Lipoxygenase Inhibitors , Platelet Aggregation Inhibitors/chemistry , Zingiberaceae/chemistry , Aldehydes/chemistry , Aldehydes/isolation & purification , Diterpenes/chemistry , Diterpenes/isolation & purification , Diterpenes/pharmacology , Drug Evaluation, Preclinical , Flowers/chemistry , Humans , Molecular Structure , Platelet Aggregation Inhibitors/isolation & purification , Platelet Aggregation Inhibitors/pharmacology , Spectrum Analysis , Structure-Activity Relationship
18.
Biosci Biotechnol Biochem ; 70(9): 2121-30, 2006 Sep.
Article in English | MEDLINE | ID: mdl-16960355

ABSTRACT

We compared the dietary effects of dihomo-gamma-linolenic acid (DGLA) contained in the DGLA oil produced by a fungus with gamma-linolenic acid (GLA) on the fatty acid composition. Wistar rats were fed with three kinds of oil for two weeks as follows: (i) control group: corn oil; (ii) GLA group: borage oil; (iii) DGLA group: DGLA oil/safflower oil = 55:45. The DGLA concentrations in the liver, serum, and brain of the DGLA group were higher than those of the GLA oil group. We also examined the dose effect of DGLA. The DGLA levels in the liver, serum, and brain significantly increased with increasing dosage of DGLA in the diet. DGLA administration significantly increased the ratio of PGE1/PGE2 in the rat plasma. The mechanism for GLA administration to improve atopic eczema is thought to involve an increase in the concentration of DGLA metabolized from GLA, so these results suggest that the dietary effect of DGLA would be more dominant than GLA.


Subject(s)
8,11,14-Eicosatrienoic Acid/pharmacokinetics , Brain/metabolism , Dermatitis, Atopic/drug therapy , Liver/metabolism , gamma-Linolenic Acid/pharmacokinetics , 8,11,14-Eicosatrienoic Acid/administration & dosage , 8,11,14-Eicosatrienoic Acid/blood , Administration, Oral , Alprostadil/blood , Animals , Delta-5 Fatty Acid Desaturase , Dinoprostone/blood , Fatty Acid Desaturases/biosynthesis , Fatty Acid Desaturases/genetics , Linoleoyl-CoA Desaturase/biosynthesis , Linoleoyl-CoA Desaturase/genetics , Male , PPAR alpha/biosynthesis , PPAR alpha/genetics , RNA, Messenger/biosynthesis , RNA, Messenger/genetics , Rats , Rats, Wistar , Reverse Transcriptase Polymerase Chain Reaction , Sterol Regulatory Element Binding Proteins/biosynthesis , Sterol Regulatory Element Binding Proteins/genetics , gamma-Linolenic Acid/administration & dosage , gamma-Linolenic Acid/blood
19.
Biosci Biotechnol Biochem ; 70(4): 958-65, 2006 Apr.
Article in English | MEDLINE | ID: mdl-16636464

ABSTRACT

The raw and boiled odors of celery leaves and stalks were investigated. Among 12 compounds identified as potent odorants, 3-n-butylphthalide 1, sedanenolide 2, and trans- and cis-sedanolides 3, 4 were assessed to be most contributive to the overall odor of celery. These three phthalides, (3E,5Z)-1,3,5-undecatriene, myrcene, and (E)-2-nonenal were common to both raw and boiled materials. Two compounds, ((Z)-3-hexenal and (Z)-3-hexenol), were dominant in raw materials and four compounds, (2-methylbutanoic acid, sotolon, beta-damascenone, and beta-ionone), were dominant in boiled materials. Sensory evaluations were performed on natural celery odor and a series of reconstructed model aromas by assigning each intensity ratings for a set of seven odor qualities which aptly describe the odors of raw and boiled celery. According to the evaluation results, six common components contributed to the moderate odor of raw celery, two components dominant in raw materials enhanced the raw celery character, and four components dominant in boiled materials reduced the raw celery character and enhanced the boiled celery character. It was clarified that boiling-induced changes in celery odor were not affected by the amounts of phthalides, but by thermally generated compounds such as sotolon, beta-damascenone, and beta-ionone, which reduce the "green spicy" note.


Subject(s)
Apium/chemistry , Benzofurans/chemistry , Plant Stems/chemistry , Smell/physiology , Adult , Benzofurans/pharmacology , Female , Hot Temperature , Humans , Male , Models, Biological , Molecular Structure , Plant Extracts/chemistry , Plant Leaves/chemistry , Smell/drug effects , Water
20.
Antioxid Redox Signal ; 7(11-12): 1621-9, 2005.
Article in English | MEDLINE | ID: mdl-16356125

ABSTRACT

We previously conducted screening tests of the chloroform extracts from a total of 89 species of Japanese plant food items for their suppressive effects on superoxide (O(2) ()) generation through both NADPH oxidase and xanthine oxidase, and reported that mioga ginger (Zingiber mioga Roscoe) indicated the strongest suppressive activities. In this study, the suppressive effects of mioga ginger constituents, aframodial, and galanal B, together with [6]-gingerol and galanolactone occurring in ginger, on free radical generation and inducible proinflammatory gene expressions were investigated. Of these constituents, aframodial (20 microM) exhibited marked suppressive effects on 12-O-tetradecanoylphorbol-13-acetate-induced O(2) () generation in HL-60 cells and lipopolysaccharide (LPS)/interferon-gamma-induced nitric oxide (NO) generation in RAW264.7 cells (inhibition rates [IRs]=84.6% and 95.9%, respectively). Aframodial also strongly suppressed the stimulated HL-60 cell-induced mutagenicity in AS52 cells (IR=95.9%). The LPS-induced expression of inducible proinflammatory genes such as inducible NO synthase, interleukin (IL)-1beta, IL-6, and granulocyte-macrophage colony-stimulating factor was significantly abolished (IRs=99.1%, 74.6%, 74.0%, and 64.4%, respectively) by aframodial. In addition, degradation of the inhibitor of nuclear factor kappaB was suppressed by this compound (IR=100%), suggesting that the suppression of nuclear factor kappaB activation, at least in part, is involved. Taken together, these results suggest that aframodial has potent antioxidative and anti-inflammatory potentials, and may be a promising candidate in prevention and/or therapy for chronic inflammationassociated carcinogenesis.


Subject(s)
Gene Expression Regulation/drug effects , Macrophages/drug effects , Macrophages/metabolism , Plant Extracts/pharmacology , Reactive Nitrogen Species/biosynthesis , Reactive Oxygen Species/metabolism , Zingiberaceae/chemistry , Animals , Catechols , Cell Differentiation , Cell Line , Diterpenes/pharmacology , Fatty Alcohols/pharmacology , Humans , I-kappa B Kinase/metabolism , Inflammation/genetics , Inflammation Mediators , Lipopolysaccharides/pharmacology , Macrophages/cytology , Mice , Naphthalenes/pharmacology , Spiro Compounds/pharmacology
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