Comparative Evaluation of the Antibacterial Efficacy of Aloe Vera, 3% Sodium Hypochlorite, and 2% Chlorhexidine Gluconate Against Enterococcus faecalis: An In Vitro Study.

Aim To compare the antibacterial efficacy of irrigants (aloe vera, 3% sodium hypochlorite (NaOCl), 2% chlorhexidine (CHX), and saline) against Enterococcus faecalis using the turbidometric analysis and colony count method. Materials and methods Eighty freshly extracted, single-rooted, human mandibular premolar teeth were taken. Access opening was done, instrumented, and autoclaved. Samples were inoculated with 10 µL of an Enterococcus faecalis (E. faecalis) bacterial suspension and incubated at 37◦C for three days. Samples were divided into four groups of 20 teeth each based on the type of irrigating solution used. Group 1 was irrigated with 3% sodium hypochlorite; Group 2 with 2% chlorhexidine; group 3 with aloe vera; and Group 4 with 0.9% saline (the control group). Ten teeth from each group were subjected to a turbidity analysis by spectrophotometer and the remaining 10 teeth from each group were tested for colony-forming units (CFU)/mL. The plates were incubated at 37◦C for 24 hours and CFU that were grown were counted using a bacterial colony counter. Results were subjected to an analysis of variance (ANOVA) followed by a post hoc Games-Howell test. Results All the tested irrigating solutions demonstrated an antibacterial effect against E. faecalis. The greatest antimicrobial effects were observed in samples treated with 2% CHX (p<0.001). No statistically significant difference was found between 3% NaOCl and aloe vera (p > 0.001) against E. faecalis. Conclusion Two percent chlorhexidine exhibited good antimicrobial efficacy against E. faecalis. Three percent NaOCl and aloe vera showed a similar antimicrobial efficacy against E. faecalis. Aloe vera can be used as an antibacterial agent in novel drugs for the treatment of bacterial diseases.

Expansion of gutta-percha in contact with various concentrations of zinc oxide-eugenol sealer: a three-dimensional volumetric study.

INTRODUCTION: Successful endodontic treatment depends on achievement of a fluid-tight seal that is not possible solely with gutta-percha and requires the use of a root canal sealer. Eugenol, which is a principal component in zinc oxide-eugenol (ZOE) based sealers, is reported to produce a volumetric expansion of gutta-percha. The aim of this study was to evaluate the three-dimensional expansion of gutta-percha at various powder/liquid ratios of ZOE-based sealer by using spiral computed tomography (SCT). METHODS: Fifty freshly extracted premolars with single canal were decoronated and instrumented by using RaCe rotary instruments (35/06). The teeth were divided into 5 groups of 10 each, and volume of the canal was measured by using SCT. The teeth were then obturated with gutta-percha cones (35/04) in groups ZE 1:1 to ZE 1:4 with Pulp Canal Sealer EWT (ZOE-based) with powder/liquid ratio of 1:1, 1:2, 1:3, and 1:4, respectively, and gutta-percha alone in control group (no sealer group). The filled volume in each canal was measured by using SCT 1 day, 7 days, and 1 month after obturation, and percentages of obturated volume and of volume changes in groups over time were calculated. The data were statistically analyzed by using one-way analysis of variance and post hoc multiple comparison tests. RESULTS: The groups ZE 1:2 and ZE 1:3 gave the highest mean volume values during a 1-month period and were significantly different in comparison with groups ZE 1:1 and ZE 1:4 (P < .05). CONCLUSIONS: Increasing the ratio of eugenol in sealer resulted in volumetric increase of gutta-percha. However, further studies should be performed to confirm the expansion and sealing ability of gutta-percha of the size corresponding to the prepared canal, leading to the achievement of fluid impervious seal.