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Clin Diagn Lab Immunol ; 7(6): 940-4, 2000 Nov.
Article in English | MEDLINE | ID: mdl-11063502

ABSTRACT

A liquid phase blocking ELISA (LPB-ELISA) was adapted for the detection and quantification of antibodies to Newcastle disease virus. Sera from vaccinated and unvaccinated commercial flocks of ostriches (Struthio camelus) and rheas (Rhea americana) were tested. The purified and nonpurified virus used as the antigen and the capture and detector antibodies were prepared and standardized for this purpose. The hemagglutination-inhibition (HI) test was regarded as the reference method. The cutoff point for the LPB-ELISA was determined by a two-graph receiver operating characteristic analysis. The LPB-ELISA titers regressed significantly (P < 0.0001) on the HI titers with a high correlation coefficient (r = 0.875). The two tests showed good agreement (kappa = 0.82; P < 0.0001), relative sensitivity (90.91%) and specificity (91.18%), and accuracy (91.02%), suggesting that they are interchangeable.


Subject(s)
Antibodies, Viral/blood , Enzyme-Linked Immunosorbent Assay/methods , Newcastle Disease/immunology , Newcastle disease virus/immunology , Animals , Enzyme-Linked Immunosorbent Assay/statistics & numerical data , Hemagglutination Inhibition Tests , Newcastle Disease/prevention & control , Rheiformes/immunology , Sensitivity and Specificity , Struthioniformes/immunology , Vaccination
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