Subject(s)
Glomus Tumor/diagnosis , Nail Diseases/diagnosis , Neurofibromatosis 1/complications , Skin Neoplasms/diagnosis , Female , Glomus Tumor/complications , Glomus Tumor/surgery , Humans , Magnetic Resonance Imaging , Middle Aged , Nail Diseases/complications , Nail Diseases/surgery , Skin Neoplasms/complications , Skin Neoplasms/surgeryABSTRACT
Prymnesin-2 is a potent red tide toxin characterized by a highly oxidized C(90) carbon chain and multiple functional groups. Succeeding the assignment of the relative stereochemistry in the polycyclic-ether segment, the absolute configurations of two chiral centers in linear chain parts were elucidated. The configuration at C14 bearing an amino group was determined to be S by using a chiral anisotropic reagent and that at chlorinated C85 to be S by fluorimetric chiral HPLC comparison between a degradation product and synthetic references.
Subject(s)
Dinoflagellida/chemistry , Glycosides/chemistry , Marine Toxins/chemistry , Pyrans/chemistry , Animals , Chromatography, High Pressure Liquid , Magnetic Resonance Spectroscopy , Molecular Conformation , Polyketides , Spectrometry, Fluorescence , Spectrometry, Mass, Electrospray Ionization , StereoisomerismABSTRACT
We have devised a mapping method for rapid assembly and ordering of bacterial artificial chromosome (BAC) clones on a radiation hybrid (RH) panel, using sequence-tagged sites (STSs) and PCR. The protocol consists of two rounds of two-dimensional screening from a limited number of BACs to correspond each to an STS. In the first round, STSs are assembled in the RH bins and ordered according to PCR signals derived from 384-well microtiter plates (MTPs) in which BAC clones have been arrayed. In the second round, individual BAC clones are isolated from the MTPs to build a contig. We applied this method to a 35-Mb region spanning human chromosome 1p35-p36 and assembled 1366 BACs in 11 contigs, the longest being about 20 Mb. The working draft sequences of the human genome have been integrated into the contigs to validate the accuracy.
Subject(s)
Chromosome Mapping/methods , Chromosomes, Human, Pair 1/genetics , Sequence Tagged Sites , Chromosomes, Artificial, Bacterial , Contig Mapping , DNA/genetics , Humans , In Situ Hybridization, FluorescenceABSTRACT
BACKGROUND: Neuroblastoma (NBL) has a distinct nature in different prognostic subgroups. PROCEDURE: To understand the molecular mechanism of NBL's genesis and biology as well as that of the neural crest development, we constructed full-length-enriched cDNA libraries by an oligo-capping method from two different subsets of primary NBL, one with favorable biology and the other with MYCN amplification. RESULTS: Sequencing analysis of these libraries revealed that the expression profile was markedly different between both subsets. To identify the genes differentially expressed between the subsets, semi-quantitative RT-PCR analyses are proceeding. CONCLUSION: So far, 54 transcripts have been found to be expressed at high levels in favorable NBLs, and significantly at low levels in unfavorable NBLs.
Subject(s)
Gene Expression Regulation, Neoplastic/genetics , Gene Library , Neuroblastoma/genetics , Humans , Mass Screening , Tumor Cells, CulturedABSTRACT
BACKGROUND: The biology of neuroblastoma (NBL) is at least partly regulated by neurotrophic factors and their receptors. PROCEDURE: To identify novel NBL-related genes that affect growth, differentiation, and programmed cell death of the tumor, we constructed full-length-enriched cDNA libraries by oligo-capping method. RESULTS: Semi-quantitative and quantitative real-time RT-PCR showed that the nbla3145 gene was significantly highly expressed in favorable subset of NBL. The nucleo-tide sequence of nbla3145 revealed that it was a novel member of metalloproteinase, endothelin-converting enzyme, and was the same gene recently reported as XCE. We also cloned nbla3145beta which was a novel truncated form of nbla3145 (or nbla3145alpha). CONCLUSIONS: Our results suggested that nbla3145/XCE plays an important role in regulating growth and differentiation of NBL.
Subject(s)
Gene Expression Regulation, Neoplastic , Gene Library , Metalloendopeptidases/genetics , Neuroblastoma/genetics , HumansABSTRACT
The marine polyether toxin, 45-hydroxyyessotoxin, was isolated together with yessotoxin from the scallop, Patinopecten yessoensis. The 45-hydroxy group in the side chain was esterified with (S)- and (R)-alpha-methoxyalpha-trifluoromethylphenylacetic acids (MTPA). A detailed analysis of the 2D NMR spectra of the two esters established the R configuration at C45.
Subject(s)
Ethers, Cyclic/chemistry , Mollusca/chemistry , Mollusk Venoms/isolation & purification , Oxocins , Animals , Models, ChemicalABSTRACT
A new brevetoxin B analog, brevetoxin B4 (BTXB4), was isolated as the major toxin in greenshell mussels, Perna canaliculus, collected in New Zealand at the time of the neurotoxic shellfish poisoning incident. The new analog accounted for nearly two-thirds of the mouse lethality of the shellfish and was determined to be a mixture of N-myristoyl-BTXB2 and N-palmitoyl-BTXB2.
