CLINICAL FEATURES OF THE CARIES COURSE OF TEMPORARY TEETH IN CHILDREN.

OBJECTIVE: The aim: Assess the incidence of caries of temporary teeth in children using the indices drxfm, SIC, ICDAS. PATIENTS AND METHODS: Materials and methods: A survey of 255 children 3 and 6 years old was conducted and 2 research groups were formed. We determined the prevalence, intensity of caries by the expanded formula drxfm, SIC-index and ICDAS II 1-6, as well as determined the Green-Vermilion hygienic index. RESULTS: Results: The high prevalence of caries of permanent teeth according to WHO criteria was revealed. The average value of ICDAS II 1-6 at the age of 3 years is 1.91 « 0.24 teeth, p <0.05. In 6-year-olds, this index was 3.78 « 0.32 teeth, p <0.05. In 3-year-old children, the average value of SIC was 2.64 « 0.09 teeth, which probably differed from that of 6-year-old children - 6.82 « 0.12 teeth. Regarding the Green-Vermilion index, unsatisfactory oral hygiene was found in children aged 3 and 6 years. CONCLUSION: Conclusions: Thus, the obtained high rates of prevalence and intensity of caries determine the special importance of caries prevention measures and indicate the need to find new approaches in the fight for dental health of children.

The diverse metabolic heterogeneity of stem cells in a BRCA+/-breast cancer population.

Breast cancers are very heterogeneous tissues constituted by epithelial cancer cells and an abnormal tumor microenvironment - cancer-associated fibroblasts (CAFs), activated adipocytes, mesenchymal stem cells (MSCs), and others. The aim of the study is to cancer cells and their microenvironment, which behave like a complex and heterogeneous metabolic ecosystem, where cancer cells can reprogram their metabolism as a result of interaction with the components of the microenvironment. The study was based on cancer stem cells (CSC) that were isolated from breast tumors by magnetic separation (AutoMACS). We used spectrophotometric methods for the measurement of aldehyde dehydrogenase (ALDH) enzymatic activity. For these experiments, we used breast cancer and normal stem cell lines. Analyses showed that the proportion of BRCA+ CSC cells was in accordance with the relatively low percentages of CSCs in BRCA+ tumors. ALHD was significantly higher in the CSCs-high BRCA+ breast cancer and CSCs-low BRCA- breast cancer cells, compared with the CSCs-low BRCA+ breast cancer. Breast cancer from BRCA mutation carriers harbor more "high-energy" cell sub-populations than "low-energy" and have their more aggressive phenotype. Key oncogenic pathways known to be dysregulated in breast cancer also regulate stem-cell behavior.

Pediatric biobanks and parents of disabled children associations opinions on establishing children repositories in developing countries.

Pediatric biobanks are an indispensable resource for the research needed to bring advances in personalized medicine into pediatric medical care. It is unclear how or when these advances in medical care may reach children, but it is unlikely that research in adults will be adequate. We conducted the screening for a hypothetic problem in various European and American pediatric biobanks based on online surveys through e-mail distribution based on the Biobank Economic Modeling Tool (BEMT) questionnaire model. Participants in the survey had work experience in biobanking for at least 3 years or more. Contact information about the survey participants was confirmed on the social networks profiles (LinkedIn), as well as on generally available websites. First, we tried creating a model which can show the pediatric preclinical and basic clinical phase relationship and demonstrate how pediatric biobanking is linked to this process. Furthermore, we tried to look for new trends, and the final goal is to put the acquired knowledge into practice, so medical experts and patients could gain usable benefit from it. We concluded that leading positions must take into account ethical and legal aspects when considering the decision to include children in the biobank collection. However, communication with parents and children is essential. The biobank characteristics influence the biobank's motives to include children in the consent procedure. Moreover, the motives to include children influence how the children are involved in the consent procedure and the extent to which children are able to make voluntary decisions as part of the consent procedure.

The condition of volumetric and osmoregulatory function of the kidneys in toxicemia of purulent-septic genesis in patients with diabetes mellitus.

OBJECTIVE: The aim of the study is to investigate the state of volumetric and osmoregulatory function of the kidneys in diabetes complicated by the syndrome of the endogenous intoxication of purulent-septic genesis. PATIENTS AND METHODS: Materials and methods: The study group was consisted of patients with insulin-dependent diabetes mellitus with complicated syndrome of the endogenous intoxication of purulent-septic genesis (CSDI). RESULTS: Results: The starting indices of volumetric and osmoregulatory function of the kidneys in patients with INCs complicated by the endogenous intoxication of purulent-septic genesis (CEI GHG) syndrome are characterized by the values of the inhibition of the volumorregulatory (by the clearance of sodium by 11%, p <0.05) and the activation of the osmoregulatory osmotically active substances by 23%, p <0.05) renal function. The volume increasing of the extracellular space by the Ringer solution activates the volumetric and osmoregulatory function of the kidneys, respectively, in patients with NSCL at 162 ± 27.1% (Δ, p <0.05) and 138 ± 48.3% (Δ, p <0.05), and at INCS complicated CEE GHS by 260 ± 47.8% (Δ, p <0.05) and 147 ± 46.9% (Δ, p <0.05). CONCLUSION: Conclusions: The low-level isotonic loads with Ringer's solution initiate the same direction of change in indices of volumetric and osmoregulatory function of the kidneys in patients with systemic inflammatory response syndrome and diabetes mellitus with complicated endogenous intoxication syndrome of purulent-septic genesis and show dissociative hyper reactivity of the volumorregulatory function in relation to the osmoregulatory one.

Clinical and immunologic assessment of a complex of therapeutic-preventive measures concerning chronic catarrhal gingivitis in children with comorbid diabetes mellitus.

OBJECTIVE: The aim of our study is to increase the efficacy of treatment of chronic catarrhal gingivitis in children suffering from type 1 diabetes mellitus by means of improvement of themethods of pharmacological correction on the basis of investigation of clinical-immunologic peculiarities of the disease. PATIENTS AND METHODS: Materials and methods: 2 groups of the study were formed. Children received basic insulin therapy. The treatment of chronic catarrhal gingivitis in children from the main group were suggested the antiseptic solution "Decasan"; pill of a probiotic action "BioGaia ProDentis" and the immune modulator "Imupret". Children from the comparative group were treated according to the common scheme. RESULTS: Results: The state of the oral hygiene in all the children after treatment improved considerably. According to PMA index inflammatory process was completely eliminated in children from the main group. A similar tendency was observed concerning sextants with gingival bleeding. In children dental calculus was lacking after treatment. Lysozyme activity in the oral fluid of children after treatment increased approximately 37.50% in the main group, and 16,67 % - in the comparison group. A similar tendency was found concerning sIgА level. CONCLUSION: Conclusions: Therefore, conducted course of the treatment concerning chronic catarrhal gingivitis promoted considerable improvement of the periodontal tissue in children.