ABSTRACT
UNLABELLED: Purpose of the study was to assess a predictive value of body mass index for perioperative hyperglycemia occurrence in cardio-surgical patients without a diabetes mellitus. MATERIALS AND METHODS: Retrospective analysis of glycemic profile, frequency and level of perioperative hyperglycemia was performed 120 patients without a diabetes mellitus, undergoing elective cardiac surgeries with cardiopulmonary bypass were included in the study. All patients were divided into three groups. Group-1 included patients with normal body weight (body mass index (BMI) < 25), Group-2--patients with increased body weight (BMI 25-29.9), Group-3--patients with obesity (BMI > 30). RESULTS: Elective cardiac surgeries with artificial circulation accompanied with episodes of hyperglycemia. Hyperglycemia occurrence did not have relation with initial glycemic profile of the patients. Glycemia level increased during surgery and the highest levels of both glycemia increasing of hyperglycemia frequency were fixed during cardiopulmonary bypass and postperfusion period. Increased body weight and obesity are predisposing causes of perioperative hyperglycemia.
Subject(s)
Body Mass Index , Hyperglycemia , Myocardial Revascularization , Perioperative Period , Postoperative Complications , Blood Glucose/analysis , Case-Control Studies , Humans , Hyperglycemia/diagnosis , Hyperglycemia/epidemiology , Hyperglycemia/etiology , Postoperative Complications/diagnosis , Postoperative Complications/epidemiology , Postoperative Complications/etiology , Predictive Value of Tests , Retrospective StudiesABSTRACT
Rearranged genes for heavy and light immunoglobulin chains have been studied in the genomes of hybridoma PTF-02 and parent myeloma P3-X63.Ag8.653. The hybridoma was shown to contain three rearranged allelic variants of the heavy chain gene's locus. Gene H2, responsible for the synthesis of the heavy chain of the antibody to transferrin, was transmitted to the hybridoma cell from a lymphocyte. The structure of the 5'-terminal codons of this gene corresponds to N-terminal amino acid residues of a heavy chain expressed by PTF-02 hybridoma. Two other genes (H1 and H3) were found both in the hybridoma and parent myeloma. Both H1 and H3 genes have serious defects in their structure and do not function. Rearranged k-genes were also identified both in the hybridoma and parent myeloma. A functional (K2) gene appeared in the hybridoma genome from an antigen-stimulated lymphocyte. The fetal mouse Vk gene, which has high homology (96.5%) with the K2 gene, was cloned and sequenced. Both K2 and the Vk fetal gene belong to one subfamily of k-genes. The rearranged gene K1 is nonfunctional because it has lost the Jk locus. This gene was transmitted from myeloma used for fusion. In such a way, the origin of all rearranged heavy and light chain genes in hybridoma PTF-02 was established.
Subject(s)
Genes, Immunoglobulin , Hybridomas , Alleles , Amino Acid Sequence , Animals , Base Sequence , Cell Line , Chromosome Mapping , DNA , DNA Probes , Gene Rearrangement , Mice , Molecular Sequence Data , Tumor Cells, CulturedABSTRACT
The system of DNA recombination in vitro was constructed. It comprises two plasmids, the derivatives of pBR322 deleted in the genes for tetracycline resistance, and the recombinogenic extract of the thymus lymphocytes nuclei of mice. The system permits to study the effect of proteins and factors on the efficiency of recombination resulting in reconstruction of the tetracycline resistance gene. Double-strand cuts in one of the deleted plasmids were necessary for recombination. Double-strand cuts by Ca/Mg-dependent endonuclease of the human spleen lymphocytes nuclei were more efficient as compared with the ones of DNAase I, restriction endonucleases PaeI and SalI in the initiation of recombination. The possible role of Ca/Mg-dependent endonuclease in recombination in vivo is discussed.
Subject(s)
DNA/genetics , Endodeoxyribonucleases/genetics , Recombination, Genetic , Spleen/enzymology , Cell Nucleus/enzymology , Humans , PlasmidsABSTRACT
The cleavage of the murine J kappa-segments by purified Ca/Mg-dependent endonuclease was studied. The enzyme was shown to cleave specifically the J kappa-segments introducing the double strand breaks in 5'-regions of the J kappa-genes. The number of thus generated discreet fragments does not change during incubation. Specificity of the enzyme is not changed by substitution of Ca/Mg for Mn. The enzyme activity is inhibited by 0.1 M NaCl as well as its specificity. Comparison of the enzyme with the known nuclease activities that cleave specifically the J kappa-segments demonstrated that the activities of the latter are determined by Ca/Mg-dependent endonuclease. The results suggest the possible participation of Ca/Mg-dependent endonuclease in Ig-genes recombination.
Subject(s)
Endodeoxyribonucleases/genetics , Genes, Immunoglobulin , Immunoglobulin kappa-Chains/genetics , Lymphocytes/enzymology , Multigene Family , Animals , Cell Nucleus/enzymology , Endodeoxyribonucleases/metabolism , Humans , Hydrolysis , MiceABSTRACT
Fragmentation of the plasmid pBR322 DNA by a purified preparation of Ca/Mg-dependent endonuclease has been studied. It was shown that on the first steps of reaction the double-stranded cuts are introduced into the superhelical DNA independent of singlestranded ones. The doublestranded cuts are introduced into superhelical and linear DNA in 12 sites enriched with GC-pairs, 9 of them include pentanucleotide CGCGG(CCGCC) that is functionally significant. Relaxation of the plasmid DNA by topoisomerase I blocks the sitespecific action of the enzyme. Ca/Mg-dependent endonuclease is concluded to be topologically dependent enzyme, possibly, participating in the recombination processes.
