ABSTRACT
Activation of inflammatory pathways in endothelial cells contributes to tumour growth and progression in multiple human cancers. Cellular adhesion molecules are involved in leukocyte recruitment to the vascular inflammatory environment where it plays a critical role in angiogenesis, suppression of apoptosis, proliferation, invasion and metastasis. We describe here the development of streptavidin-coated polyurethane-urea nanoparticles as multifunctional nanocarriers for fluorescence imaging or targeting of the tumour environment to identify and arrest the vascular network irrigating the tumour tissue. The design of these multifunctional nanoparticles involves incorporating streptavidin to the nanoparticle polymeric matrix. The obtained nanoparticles are spherical and exhibit an average diameter of 70-74 nm. Streptavidin-coated nanoparticles spontaneously bind biotinylated antibodies against VCAM-1 and ICAM-1 which in vitro and in vivo specifically attached to inflamed endothelial cells. Indeed the incorporation of CBO-P11 (a specific inhibitor of the vascular endothelial growth factor proangiogenic and proinflammatory pathway) to these nanoparticles allows a targeted pharmacological effect thereby decreasing the proliferation only in inflamed endothelial cells. The multiple functionalisation strategy described here could be exploited for tumour diagnostics or targeted drug delivery to tumour vasculature with a good safety profile and an attractive array of possibilities for biomedical applications.
Subject(s)
Angiogenesis Inhibitors/administration & dosage , Drug Carriers/administration & dosage , Endothelial Growth Factors/administration & dosage , Nanoparticles/administration & dosage , Neoplasms/drug therapy , Neovascularization, Pathologic/drug therapy , Peptides, Cyclic/administration & dosage , Angiogenesis Inhibitors/chemistry , Animals , Antibodies/immunology , Biotin/chemistry , Cell Proliferation/drug effects , Drug Carriers/chemistry , Endothelial Growth Factors/chemistry , Human Umbilical Vein Endothelial Cells/drug effects , Human Umbilical Vein Endothelial Cells/metabolism , Humans , Intercellular Adhesion Molecule-1/immunology , Lipopolysaccharides , Mice , Nanoparticles/chemistry , Neoplasms/blood supply , Neoplasms/diagnosis , Neoplasms/pathology , Neovascularization, Pathologic/diagnosis , Peptides, Cyclic/chemistry , Polyurethanes/chemistry , Streptavidin/chemistry , Vascular Cell Adhesion Molecule-1/immunology , Vascular Endothelial Growth Factor Receptor-2/antagonists & inhibitorsABSTRACT
La formación y profesionalización de las tareas docentes es una de las respuestas a las exigencias decalidad y excelencia docentes. Ser un profesional docente eficiente y eficaz requiere la adquisición deunas competencias que permitan ejercer su función adecuadamente. La planificación de una nuevaasignatura en el marco del Espacio Europeo de Educación Superior (EEES) supone un reto que hayque asumir para realizar una docencia de calidad. La puesta en marcha de una nueva asignatura es unagran oportunidad para reflexionar individualmente y con otros profesores sobre la práctica docente ypara profundizar en procesos de innovación en metodología docente, en los recursos a utilizar, en lossistemas de evaluación, en la definición de objetivos y en la selección de contenidos.El objetivo del trabajo realizado consistió en aplicar los conocimientos adquiridos en el Máster enDocencia Universitaria para profesorado novel a la elaboración del plan docente de la asignaturaFarmacia Galénica I que se impartirá en el sexto semestre del Grado de Farmacia en la Universidadde Barcelona. El plan docente que se ha elaborado ha tenido como referente las competencias de latitulación y los contenidos se han delimitado en relación a los objetivos formativos de la asignatura.Las estrategias metodológicas propuestas y la evaluación del proceso de aprendizaje se han adaptado ala docencia de grupos muy numerosos (80-100 alumnos), intentando favorecer la autonomía delalumnado(AU)
The training and professionalization of the educational tasks is one of the answers to the educationalrequirements of quality and excellence. To be an efficient and effective professional need theacquisition of a few competences that allows exercising his function adequately. The planning of anew subject in the frame of the European Higher Education Area (EHEA) supposes a challenge that itis necessary to take up to manage a high quality teaching. The development of a new subject is a greatopportunity to think individually and with other colleagues on the educational practice and to penetrateinto processes of innovation into educational methodology, in the higher education resources, in theevaluation, in the definition of aims and in the selection of contents.The aim of the present work consisted of applying the knowledge acquired in the Master in "HigherEducation Teaching for young teachers" to the elaboration of the learning plan of the subject"Farmacia Galénica I" that will be given in the sixth semester of the Degree of Pharmacy in theUniversity of Barcelona. The learning plan that has been elaborated has taken the competences of thedegree in Pharmacy as a model and the contents have been delimited in relation to the formative aimsof the subject. The methodological strategies and the evaluation of the learning process have beenadapted to the teaching of groups with 80-100 students, trying to help the autonomy of the student(AU)
