Subject(s)
Bacillus anthracis/genetics , Bacillus anthracis/isolation & purification , Polymerase Chain Reaction/methods , Animals , Anthrax/diagnosis , Anthrax/microbiology , Bacillus anthracis/classification , Bioterrorism , Environmental Monitoring , Genes, Bacterial , Genotype , Humans , Reproducibility of Results , Sensitivity and Specificity , Time FactorsABSTRACT
The complete nucleotide sequences of eight West Nile (WN) virus strains (Egypt 1951, Romania 1996-MQ, Italy 1998-equine, New York 1999-equine, MD 2000-crow265, NJ 2000MQ5488, NY 2000-grouse3282, and NY 2000-crow3356) were determined. Phylogenetic trees were constructed from the aligned nucleotide sequences of these eight viruses along with all other previously published complete WN virus genome sequences. The phylogenetic trees revealed the presence of two genetic lineages of WN viruses. Lineage 1 WN viruses have been isolated from the northeastern United States, Europe, Israel, Africa, India, Russia, and Australia. Lineage 2 WN viruses have been isolated only in sub-Saharan Africa and Madagascar. Lineage 1 viruses can be further subdivided into three monophyletic clades.
