Tracking Vibrio: population dynamics and ecology of Vibrio parahaemolyticus and V. vulnificus in an Alabama estuary.

Vibrio is a genus of halophilic, gram-negative bacteria found in estuaries around the globe. Integral parts of coastal cultures often involve contact with vectors of pathogenic Vibrio spp. (e.g., consuming raw shellfish). High rates of mortality from certain Vibrio spp. infections demonstrate the need for an improved understanding of Vibrio spp. dynamics in estuarine regions. Our study assessed meteorological, hydrographic, and biological correlates of Vibrio parahaemolyticus and V. vulnificus at 10 sites in the Eastern Mississippi Sound System (EMSS) from April to October 2019. During the sampling period, median abundances of V. parahaemolyticus and V. vulnificus were 2.31 log MPN/L and 2.90 log MPN/L, respectively. Vibrio spp. dynamics were largely driven by site-based variation, with sites closest to freshwater inputs having the highest abundances. The E-W wind scalar, which affects Ekman transport, was a novel Vibrio spp. correlate observed. A potential salinity effect on bacterial-particle associations was identified, where V. vulnificus was associated with larger particles in conditions outside of their optimal salinity. Additionally, V. vulnificus abundances were correlated to those of harmful algal species that did not dominate community chlorophyll. Correlates from this study may be used to inform the next iteration of regionally predictive Vibrio models and may lend additional insight to Vibrio spp. ecology in similar systems. IMPORTANCE: Vibrio spp. are bacteria found in estuaries worldwide; some species can cause illness and infections in humans. Relationships between Vibrio spp. abundance, salinity, and temperature are well documented, but correlations to other environmental parameters are less understood. This study identifies unique correlates (e.g., E-W wind scalar and harmful algal species) that could potentially inform the next iteration of predictive Vibrio models for the EMSS region. Additionally, these correlates may allow existing environmental monitoring efforts to be leveraged in providing data inputs for future Vibrio risk models. An observed correlation between salinity and V. vulnificus/particle-size associations suggests that predicted environmental changes may affect the abundance of Vibrio spp. in certain reservoirs, which may alter which vectors present the greatest vibrio risk.

Education Racial and Gender Disparities in COVID-19 Worry, Stress, and Food Insecurities across Undergraduate Biology Students at a Southeastern University.

The global spread of the novel coronavirus first reported in December 2019 led to drastic changes in the social and economic dynamics of everyday life. Nationwide, racial, gender, and geographic disparities in symptom severity, mortality, and access to health care evolved, which impacted stress and anxiety surrounding COVID-19. On university campuses, drastic shifts in learning environments occurred as universities shifted to remote instruction, which further impacted student mental health and anxiety. Our study aimed to understand how students from diverse backgrounds differ in their worry and stress surrounding COVID-19 upon return to hybrid or in-person classes during the Fall of 2020. Specifically, we addressed the differences in COVID-19 worry, stress response, and COVID-19-related food insecurity related to race/ethnicity (Indigenous American, Asian/Asian American, black/African American, Latinx/Hispanic, white, or multiple races), gender (male, female, and gender expressive), and geographic origin (ranging from rural to large metropolitan areas) of undergraduate students attending a regional-serving R2 university, in the southeastern U.S. Overall, we found significance in worry, food insecurity, and stress responses with females and gender expressive individuals, along with Hispanic/Latinx, Asian/Asian American, and black/African American students. Additionally, students from large urban areas were more worried about contracting the virus compared to students from rural locations. However, we found fewer differences in self-reported COVID-related stress responses within these students. Our findings can highlight the disparities among students' worry based on gender, racial differences, and geographic origins, with potential implications for mental health of university students from diverse backgrounds. Our results support the inclusion of diverse voices in university decisioning making around the transition through the COVID-19 pandemic.

Brillouin spectroscopy of a hybrid silicon-chalcogenide waveguide with geometrical variations.

Recent advances in design and fabrication of photonic-phononic waveguides have enabled stimulated Brillouin scattering in silicon-based platforms such as underetched silicon waveguides and hybrid waveguides. Due to the sophisticated design and, more importantly, high sensitivity of the Brillouin resonances to geometrical variations in micro- and nano-scale structures, it is necessary to have access to the localized opto-acoustic response along those waveguides to monitor their uniformity and maximize their interaction strength. In this Letter, we design and fabricate photonic-phononic waveguides with a deliberate width variation on a hybrid silicon-chalcogenide photonic chip and confirm the effect of the geometrical variation on the localized Brillouin response using a distributed Brillouin measurement.

Lasing in ring resonators by stimulated Brillouin scattering in the presence of nonlinear loss.

We theoretically investigate lasing due to stimulated Brillouin scattering in integrated ring resonators. We give analytic expressions and numerical calculations for the lasing threshold for rings in the presence of for both linear and nonlinear loss. We demonstrate the operation of the ring in the different regimes of amplification and lasing, and show how these regimes depend on the coupling to the ring and on the nonlinear parameters. In the case of nonlinear losses, we find that there can exist an upper threshold to the lasing regime where the losses are dominated by free-carrier absorption. We also find that nonlinear losses can inhibit Brillouin lasing entirely for certain ranges of coupling parameters, and we show how the correct ranges of coupling parameters can be calculated and optimized for the design of integrated Brillouin lasers.

High-resolution, on-chip RF photonic signal processor using Brillouin gain shaping and RF interference.

Integrated microwave photonics has strongly emerged as a next-generation technology to address limitations of conventional RF electronics for wireless communications. High-resolution RF signal processing still remains a challenge due to limitations in technology that offer sub-GHz spectral resolution, in particular at high carrier frequencies. In this paper, we present an on-chip high-resolution RF signal processor, capable of providing high-suppression spectral filtering, large phase shifts and ns-scale time delays. This was achieved through tailoring of the Brillouin gain profiles using Stokes and anti-Stokes resonances combined with RF interferometry on a low-loss photonic chip with strong opto-acoustic interactions. Using an optical power of <40 mW, reconfigurable filters with a bandwidth of ~20 MHz and an extinction ratio in excess of 30 dB are synthesized. Through the concept of vector addition of RF signals we demonstrate, almost an order of magnitude amplification in the phase and delay compared to devices purely based upon the slow-light effect of Brillouin scattering. This concept allows for versatile and power-efficient manipulation of the amplitude and phase of RF signals on a photonic chip for applications in wireless communications including software defined radios and beam forming.

Four-wave mixing and nonlinear losses in thick silicon waveguides.

We experimentally investigate four-wave mixing and nonlinear losses in low-loss 3 µm thick silicon strip waveguides. Adiabatic bends allow for single-mode operation in an ultra-compact 35 cm long spiral. The waveguides exhibited reduced nonlinear losses due to the large mode area of 2.75 µm2. The nonlinear coefficient γ was measured as 5.5 m-1 W-1. These features, along with the low propagation loss of 0.17 dB/cm, enable large idler power generation at 1550 nm.

Tailoring of the Brillouin gain for on-chip widely tunable and reconfigurable broadband microwave photonic filters.

An unprecedented Brillouin gain of 44 dB in a photonic chip enables the realization of broadly tunable and reconfigurable integrated microwave photonic filters. More than a decade bandwidth reconfigurability from 30 up to 440 MHz, with a passband ripple <1.9 dB is achieved by tailoring the Brillouin pump. The filter central frequency is continuously tuned up to 30 GHz with no degradation of the passband response, which is a major improvement over electronic filters. Furthermore, we demonstrate pump tailoring to realize multiple bandpass filters with different bandwidths and central frequencies, paving the way for multiple on-chip microwave filters and channelizers.

Independent manipulation of the phase and amplitude of optical sidebands in a highly-stable RF photonic filter.

Microwave photonic cancellation notch filters have been shown capable of achieving ultra-high suppressions independently from the strength of optical resonant filter they use, making them an attractive candidate for on-chip signal processing. Their operation, based on destructive interference in the electrical domain, requires precise control of the phase and amplitude of the optical modulation sidebands. To date, this was attainable only through the use of dual-parallel Mach-Zehnder modulators which suffer from bias drifts that prevent stable filter operation. Here we propose a new cancellation filter topology with ease of control and enhanced stability using a bias-free phase modulator and a reconfigurable optical processor as the modulation sidebands spectral shaper. We experimentally verify the long term stability of the novel filter topology through continuous real-time monitoring of the filter peak suppression over 24 hours.

Tunable narrowband microwave photonic filter created by stimulated Brillouin scattering from a silicon nanowire.

We demonstrate the first, to the best of our knowledge, functional signal processing device based on stimulated Brillouin scattering in a silicon nanowire. We use only 1 dB of on-chip stimulated Brillouin scattering gain to create an RF photonic notch filter with 48 dB of suppression, 98 MHz linewidth, and 6 GHz frequency tuning. This device has potential applications in on-chip microwave signal processing and establishes the foundation for the first CMOS-compatible high-performance RF photonic filter.

Frequency agile microwave photonic notch filter with anomalously high stopband rejection.

We report a novel class microwave photonic (MWP) notch filter with a very narrow isolation bandwidth (10 MHz), an ultrahigh stopband rejection (>60 dB), a wide frequency tuning (1-30 GHz), and flexible bandwidth reconfigurability (10-65 MHz). This performance is enabled by a new concept of sideband amplitude and phase controls using an electro-optic modulator and an optical filter. This concept enables energy efficient operation in active MWP notch filters, and opens up a pathway toward enabling low-power nanophotonic devices as high-performance RF filters.

Si3N4 ring resonator-based microwave photonic notch filter with an ultrahigh peak rejection.

We report a simple technique in microwave photonic (MWP) signal processing that allows the use of an optical filter with a shallow notch to exhibit a microwave notch filter with anomalously high rejection level. We implement this technique using a low-loss, tunable Si3N4 optical ring resonator as the optical filter, and achieved an MWP notch filter with an ultra-high peak rejection > 60 dB, a tunable high resolution bandwidth of 247-840 MHz, and notch frequency tuning of 2-8 GHz. To our knowledge, this is a record combined peak rejection and resolution for an integrated MWP filter.