Subject(s)
Alleles , DNA Primers/analysis , Polymerase Chain Reaction/methods , Rodentia/classification , Animals , Cattle , DNA/analysis , DNA/isolation & purification , Horses , Prealbumin/genetics , RNA, Ribosomal/genetics , Receptors, Somatotropin/genetics , Rodentia/genetics , Sequence Alignment , Sus scrofaABSTRACT
Starch gel electrophoresis and histochemical staining with L-leucylglycyl-glycine revealed genetic polymorphism in peptidase B in cattle erythrocytes. Genetic analysis of 164 full families indicated that the three phenotypes observed are the product of an autosomal locus with two codominant alleles, PepB1 and PepB2. A sample consisting of 2630 individuals representing 5 Zebu, 13 European, and 2 crossbred breeds was studied. This polymorphism was observed in all Zebu and crossbred breeds. The frequency of the PepB1 allele was 0.89, 0.83, 0.59, 0.82 and 0.62 in the Nellore, Gyr, Guzerat, Indubrazil, and Tabapuan breeds, respectively. The PepB2 allele appears to be fixed in the European breeds, except Marchigiana and Chianina.
Subject(s)
Aminopeptidases/genetics , Erythrocytes/enzymology , Isoenzymes/genetics , Polymorphism, Genetic , Alleles , Aminopeptidases/metabolism , Animals , Cattle , Dipeptidyl-Peptidases and Tripeptidyl-Peptidases , Electrophoresis, Starch Gel , Genes, Dominant , Isoenzymes/metabolism , Phenotype , Species Specificity , Substrate SpecificityABSTRACT
1. Two nucleoside phosphorylase (NP) phenotypes were detected in 844 animals from four distinct genetic groups of Bos taurus and Bos indicus derivation. 2. Bos indicus breeds like Guzerat (Kankrej), Gir, Nellore (Ongole) and Indubrazil presented an NP-H frequency of 1.00, 0.928, 0.776 and 0.754 respectively, while the Canchim breed, a Bos taurus-Bos indicus crossbred cattle (5/8 Charolais-3/8 Zebu) presented a frequency of 0.372. 3. The high frequency detected from the NP-H allele in the Bos indicus breeds strongly suggests that this enzyme is a genetic marker for cattle and that it probably has a very high frequency in all Indian breeds.
