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1.
Mucosal Immunol ; 11(1): 120-130, 2018 01.
Article in English | MEDLINE | ID: mdl-28401939

ABSTRACT

The multidrug resistance-1 (MDR1) gene encodes an ATP-dependent efflux transporter that is highly expressed in the colon. In mice, loss of MDR1 function results in colitis with similarities to human inflammatory bowel diseases (IBD). Here, we show that MDR1 has an unexpected protective role for the mitochondria where MDR1 deficiency results in mitochondrial dysfunction with increased mitochondrial reactive oxygen species (mROS) driving the development of colitis. Exogenous induction of mROS accelerates, while inhibition attenuates colitis in vivo; these effects are amplified in MDR1 deficiency. In human IBD, MDR1 is negatively correlated to SOD2 gene expression required for mROS detoxification. To provide direct evidential support, we deleted intestinal SOD2 gene in mice and showed an increased susceptibility to colitis. We exploited the genome-wide association data sets and found many (∼5%) of IBD susceptibility genes with direct roles in regulating mitochondria homeostasis. As MDR1 primarily protects against xenotoxins via its efflux function, our findings implicate a distinct mitochondrial toxin+genetic susceptibility interaction leading to mitochondrial dysfunction, a novel pathogenic mechanism that could offer many new therapeutic opportunities for IBD.


Subject(s)
ATP Binding Cassette Transporter, Subfamily B, Member 1/metabolism , Colitis/genetics , Inflammation/genetics , Inflammatory Bowel Diseases/genetics , Intestines/immunology , Mitochondria/physiology , Superoxide Dismutase/genetics , Animals , Disease Models, Animal , Genetic Predisposition to Disease , Homeostasis , Humans , Metabolic Detoxication, Phase I/genetics , Mice , Mice, Inbred C57BL , Mice, Knockout , Reactive Oxygen Species/metabolism
2.
Sci Immunol ; 2(8)2017 Feb 10.
Article in English | MEDLINE | ID: mdl-28386604

ABSTRACT

Hypoxia and bacterial infection frequently co-exist, in both acute and chronic clinical settings, and typically result in adverse clinical outcomes. To ameliorate this morbidity, we investigated the interaction between hypoxia and the host response. In the context of acute hypoxia, both S. aureus and S. pneumoniae infections rapidly induced progressive neutrophil mediated morbidity and mortality, with associated hypothermia and cardiovascular compromise. Preconditioning animals through longer exposures to hypoxia, prior to infection, prevented these pathophysiological responses and profoundly dampened the transcriptome of circulating leukocytes. Specifically, perturbation of HIF pathway and glycolysis genes by hypoxic preconditioning was associated with reduced leukocyte glucose utilisation, resulting in systemic rescue from a global negative energy state and myocardial protection. Thus we demonstrate that hypoxia preconditions the innate immune response and determines survival outcomes following bacterial infection through suppression of HIF-1α and neutrophil metabolism. The therapeutic implications of this work are that in the context of systemic or tissue hypoxia therapies that target the host response could improve infection associated morbidity and mortality.

3.
Diabetologia ; 54(9): 2392-403, 2011 Sep.
Article in English | MEDLINE | ID: mdl-21667214

ABSTRACT

AIMS/HYPOTHESIS: Urocortins are the endogenous ligands for the corticotropin-releasing factor receptor type 2 (CRFR2), which is implicated in regulating energy balance and/or glucose metabolism. We determined the effects of chronic CRFR2 activation on metabolism in vivo, by generating and phenotyping transgenic mice overproducing the specific CRFR2 ligand urocortin 3. METHODS: Body composition, glucose metabolism, insulin sensitivity, energy efficiency and expression of key metabolic genes were assessed in adult male urocortin 3 transgenic mice (Ucn3(+)) under control conditions and following an obesogenic high-fat diet (HFD) challenge. RESULTS: Ucn3(+) mice had increased skeletal muscle mass with myocyte hypertrophy. Accelerated peripheral glucose disposal, increased respiratory exchange ratio and hypoglycaemia on fasting demonstrated increased carbohydrate metabolism. Insulin tolerance and indices of insulin-stimulated signalling were unchanged, indicating these effects were not mediated by increased insulin sensitivity. Expression of the transgene in Crfr2 (also known as Crhr2)-null mice negated key aspects of the Ucn3(+) phenotype. Ucn3(+) mice were protected from the HFD-induced hyperglycaemia and increased adiposity seen in control mice despite consuming more energy. Expression of uncoupling proteins 2 and 3 was higher in Ucn3(+) muscle, suggesting increased catabolic processes. IGF-1 abundance was upregulated in Ucn3(+) muscle, providing a potential paracrine mechanism in which urocortin 3 acts upon CRFR2 to link the altered metabolism and muscular hypertrophy observed. CONCLUSIONS/INTERPRETATION: Urocortin 3 acting on CRFR2 in skeletal muscle of Ucn3(+) mice results in a novel metabolically favourable phenotype, with lean body composition and protection against diet-induced obesity and hyperglycaemia. Urocortins and CRFR2 may be of interest as potential therapeutic targets for obesity.


Subject(s)
Dietary Fats/adverse effects , Hyperglycemia/metabolism , Hyperglycemia/prevention & control , Obesity/metabolism , Obesity/prevention & control , Urocortins/genetics , Urocortins/metabolism , Animals , Body Composition/drug effects , Body Composition/physiology , Dietary Fats/pharmacology , Disease Models, Animal , Energy Metabolism/drug effects , Energy Metabolism/physiology , Glucose/metabolism , Insulin/blood , Insulin-Like Growth Factor I/metabolism , Male , Mice , Mice, Inbred BALB C , Mice, Inbred C57BL , Mice, Knockout , Mice, Transgenic , Muscle, Skeletal/metabolism , Muscle, Skeletal/pathology , Phenotype , Receptors, Corticotropin-Releasing Hormone/deficiency , Receptors, Corticotropin-Releasing Hormone/genetics , Receptors, Corticotropin-Releasing Hormone/metabolism
4.
FASEB J ; 22(11): 3896-907, 2008 Nov.
Article in English | MEDLINE | ID: mdl-18697839

ABSTRACT

Glucocorticoid hormones are critical to respond and adapt to stress. Genetic variations in the glucocorticoid receptor (GR) gene alter hypothalamic-pituitary-adrenal (HPA) axis activity and associate with hypertension and susceptibility to metabolic disease. Here we test the hypothesis that reduced GR density alters blood pressure and glucose and lipid homeostasis and limits adaption to obesogenic diet. Heterozygous GR(betageo/+) mice were generated from embryonic stem (ES) cells with a gene trap integration of a beta-galactosidase-neomycin phosphotransferase (betageo) cassette into the GR gene creating a transcriptionally inactive GR fusion protein. Although GR(betageo/+) mice have 50% less functional GR, they have normal lipid and glucose homeostasis due to compensatory HPA axis activation but are hypertensive due to activation of the renin-angiotensin-aldosterone system (RAAS). When challenged with a high-fat diet, weight gain, adiposity, and glucose intolerance were similarly increased in control and GR(betageo/+) mice, suggesting preserved control of intermediary metabolism and energy balance. However, whereas a high-fat diet caused HPA activation and increased blood pressure in control mice, these adaptions were attenuated or abolished in GR(betageo/+) mice. Thus, reduced GR density balanced by HPA activation leaves glucocorticoid functions unaffected but mineralocorticoid functions increased, causing hypertension. Importantly, reduced GR limits HPA and blood pressure adaptions to obesogenic diet.


Subject(s)
Blood Pressure/drug effects , Diet/adverse effects , Dietary Fats/adverse effects , Hypertension/metabolism , Hypothalamo-Hypophyseal System/metabolism , Pituitary-Adrenal System/metabolism , Receptors, Glucocorticoid/metabolism , Adiposity/drug effects , Adiposity/genetics , Aldosterone/metabolism , Angiotensins/metabolism , Animals , Blood Glucose/metabolism , Cell Line , Dietary Fats/pharmacology , Glucose Intolerance/genetics , Glucose Intolerance/metabolism , Humans , Hypertension/chemically induced , Hypertension/genetics , Lipid Metabolism/genetics , Mice , Mice, Transgenic , Receptors, Glucocorticoid/genetics , Renin/metabolism , Weight Gain/drug effects , Weight Gain/genetics
5.
Endocrinology ; 149(4): 1861-8, 2008 Apr.
Article in English | MEDLINE | ID: mdl-18174284

ABSTRACT

Glucocorticoid excess promotes visceral obesity and cardiovascular disease. Similar features are found in the highly prevalent metabolic syndrome in the absence of high levels of systemic cortisol. Although elevated activity of the glucocorticoid-amplifying enzyme 11beta-hydroxysteroid dehydrogenase type 1 (11beta-HSD1) within adipocytes might explain this paradox, the potential role of 11beta-HSD1 in preadipocytes is less clear; human omental adipose stromal vascular (ASV) cells exhibit 11beta-dehydrogenase activity (inactivation of glucocorticoids) probably due to the absence of cofactor provision by hexose-6-phosphate dehydrogenase. To clarify the depot-specific impact of 11beta-HSD1, we assessed whether preadipocytes in ASV from mesenteric (as a representative of visceral adipose tissue) and sc tissue displayed 11beta-HSD1 activity in mice. 11beta-HSD1 was highly expressed in freshly isolated ASV cells, predominantly in preadipocytes. 11beta-HSD1 mRNA and protein levels were comparable between ASV and adipocyte fractions in both depots. 11beta-HSD1 was an 11beta-reductase, thus reactivating glucocorticoids in ASV cells, consistent with hexose-6-phosphate dehydrogenase mRNA expression. Unexpectedly, glucocorticoid reactivation was higher in intact mesenteric ASV cells despite a lower expression of 11beta-HSD1 mRNA and protein (homogenate activity) levels than sc ASV cells. This suggests a novel depot-specific control over 11beta-HSD1 enzyme activity. In vivo, high-fat diet-induced obesity was accompanied by increased visceral fat preadipocyte differentiation in wild-type but not 11beta-HSD1(-/-) mice. The results suggest that 11beta-HSD1 reductase activity is augmented in mouse mesenteric preadipocytes where it promotes preadipocyte differentiation and contributes to visceral fat accumulation in obesity.


Subject(s)
11-beta-Hydroxysteroid Dehydrogenase Type 1/physiology , Adipocytes/enzymology , Intra-Abdominal Fat/metabolism , Obesity/etiology , Stem Cells/enzymology , 11-beta-Hydroxysteroid Dehydrogenase Type 1/analysis , 11-beta-Hydroxysteroid Dehydrogenase Type 1/genetics , Animals , Cyclin-Dependent Kinase Inhibitor p27/analysis , Dietary Fats/administration & dosage , Male , Mice , Mice, Inbred C57BL , RNA, Messenger/analysis
6.
Clin Endocrinol (Oxf) ; 65(1): 81-7, 2006 Jul.
Article in English | MEDLINE | ID: mdl-16817824

ABSTRACT

OBJECTIVE: Glucocorticoid hyperactivity in adipose tissue, due to up-regulation of local glucocorticoid reactivation by 11beta-hydroxysteroid dehydrogenase-1 (11HSD1) or of glucocorticoid receptors (GR), may underpin susceptibility to the metabolic syndrome. This hypothesis has been tested extensively in subcutaneous adipose tissue (SAT) but inadequately in visceral adipose tissue (VAT). The aim of the study was therefore to examine expression of 11HSD1, GRalpha and hexose-6-phosphate dehydrogenase (H6PDH), which supplies cofactor for 11HSD1, in abdominal adipose tissue compartments and to characterize their relation to metabolic syndrome parameters. DESIGN AND SUBJECTS: A cross-sectional study including 26 premenopausal South African women. MEASUREMENTS: Biopsies were taken for measurement of mRNA levels by real-time polymerase chain reaction (RT-PCR) and 11HSD1 activity from VAT, and deep and superficial SAT compartments during elective surgery. Prior to surgery, blood pressure, blood lipid profile, body composition [by dual X-ray absorptiometry (DEXA) scan], body fat distribution [by computed tomography (CT) scan], and glucose tolerance were determined. RESULTS: 11HSD1 activity (P < 0.01) was higher in VAT than SAT, but 11HSD1 and GRalpha mRNA levels were not statistically different between compartments. 11HSD1 mRNA levels in superficial SAT correlated with VAT volume (R = 0.57, P < 0.01), insulin sensitivity calculated from the oral glucose tolerance test (OGTT) (R = -0.52, P < 0.016) and blood pressure (R = 0.48, P < 0.016). Apart from a correlation between deep SAT 11HSD1 activity and blood pressure (R = 0.72, P < 0.01), glucocorticoid action in deep SAT and VAT depots was not significantly associated with any metabolic syndrome parameters. CONCLUSION: Increased capacity for glucocorticoid regeneration in superficial SAT but not VAT is associated with visceral adiposity and other features of the metabolic syndrome in women.


Subject(s)
Glucocorticoids/metabolism , Metabolic Syndrome/metabolism , Premenopause/metabolism , Subcutaneous Fat/metabolism , 11-beta-Hydroxysteroid Dehydrogenases/genetics , 11-beta-Hydroxysteroid Dehydrogenases/metabolism , Adult , Body Composition , Carbohydrate Dehydrogenases/genetics , Carbohydrate Dehydrogenases/metabolism , Cross-Sectional Studies , Female , Glucose Tolerance Test , Humans , Hydrocortisone/urine , Intra-Abdominal Fat/metabolism , Middle Aged , RNA, Messenger/analysis , Receptors, Glucocorticoid/genetics , Receptors, Glucocorticoid/metabolism , Regression Analysis , Reverse Transcriptase Polymerase Chain Reaction , South Africa
7.
Soc Sci Med ; 63(1): 137-50, 2006 Jul.
Article in English | MEDLINE | ID: mdl-16436309

ABSTRACT

Does commitment to an ethnic identity enhance or jeopardize psychological well-being? Using data from a study of Southeast Asian "Boat People", this study examined the mental health effects of ethnic identification as the former refugees confronted common resettlement stressors in Canada--unemployment, discrimination and lack of fluency in the dominant society language. The study team administered a questionnaire to 647 respondents covering ethnic identification, demographic and employment information, language fluency, experiences with discrimination, and depressive affect. Context helped determine the relationship between ethnic identification and depressive affect. When the Southeast Asians encountered racial discrimination or unemployment, ethnic identity attachment amplified the risk of depressive affect. By contrast, a strongly held ethnic identity provided a psychological advantage for individuals experiencing difficulties with the dominant language.


Subject(s)
Adaptation, Psychological , Depressive Disorder/ethnology , Refugees/psychology , Social Identification , Stress, Psychological/ethnology , Acculturation , Adult , Aged , Aged, 80 and over , Asia, Southeastern/ethnology , Canada/epidemiology , Communication Barriers , Depressive Disorder/psychology , Female , Follow-Up Studies , Humans , Male , Middle Aged , Prejudice , Regression Analysis , Stress, Psychological/psychology , Unemployment/psychology
8.
Int J Obes Relat Metab Disord ; 28(1): 129-36, 2004 Jan.
Article in English | MEDLINE | ID: mdl-14557827

ABSTRACT

OBJECTIVES: To investigate whether administration of leptin to rats during pregnancy and lactation affects placental 11beta-hydroxysteroid dehydrogenase (11beta-HSD2) activity and the susceptibility of their offspring to weight gain and insulin resistance. DESIGN: Pregnant rats fed on a low-protein diet were administered leptin or saline by subcutaneous minipump from day 14 of gestation and throughout lactation. A further group was fed a normal diet and given saline. After weaning, the offspring of each group were fed on a normal diet until 6 weeks of age and then half of each group was transferred to a high-fat diet until 12 months of age. RESULTS: Plasma leptin levels were raised two-fold on days 16-18 of pregnancy in the leptin-treated dams, but, despite a constant rate of infusion, at parturition they dipped to control levels before rising again. The activity of placental 11beta-HSD2 was reduced by the low-protein diet; this reduction was prevented by treating the dams with leptin. The male offspring of the saline-treated dams gained more weight and had higher plasma leptin levels on the high fat than the chow diet, but the offspring of the leptin-treated dams did not. Fasting blood glucose and intraperitoneal glucose tolerance at 6 and 12 months of age was unaffected by the high-fat diet, but only the offspring of the leptin-treated dams achieved this control without raised insulin levels. CONCLUSIONS: The rate of leptin clearance appears to increase at parturition. The administration of leptin to rats during late pregnancy and lactation makes their male offspring less susceptible to high-fat-diet-induced weight gain and insulin resistance.


Subject(s)
Birth Weight/drug effects , Insulin Resistance/physiology , Lactation/physiology , Leptin/physiology , Weight Gain/drug effects , Animals , Blood Glucose , Diet, Protein-Restricted , Dietary Fats/administration & dosage , Female , Leptin/administration & dosage , Organ Size , Placenta/anatomy & histology , Pregnancy , Rats , Rats, Wistar
9.
Science ; 294(5549): 2166-70, 2001 Dec 07.
Article in English | MEDLINE | ID: mdl-11739957

ABSTRACT

The adverse metabolic consequences of obesity are best predicted by the quantity of visceral fat. Excess glucocorticoids produce visceral obesity and diabetes, but circulating glucocorticoid levels are normal in typical obesity. Glucocorticoids can be produced locally from inactive 11-keto forms through the enzyme 11beta hydroxysteroid dehydrogenase type 1 (11beta HSD-1). We created transgenic mice overexpressing 11beta HSD-1 selectively in adipose tissue to an extent similar to that found in adipose tissue from obese humans. These mice had increased adipose levels of corticosterone and developed visceral obesity that was exaggerated by a high-fat diet. The mice also exhibited pronounced insulin-resistant diabetes, hyperlipidemia, and, surprisingly, hyperphagia despite hyperleptinemia. Increased adipocyte 11beta HSD-1 activity may be a common molecular etiology for visceral obesity and the metabolic syndrome.


Subject(s)
Adipose Tissue/enzymology , Disease Models, Animal , Hydroxysteroid Dehydrogenases/metabolism , Metabolic Syndrome , Obesity/enzymology , Obesity/genetics , 11-beta-Hydroxysteroid Dehydrogenase Type 1 , Abdomen , Adipocytes/cytology , Adipocytes/pathology , Adipose Tissue/metabolism , Animals , Body Composition , Cell Size , Corticosterone/blood , Corticosterone/metabolism , Dietary Fats/administration & dosage , Eating , Gene Targeting , Humans , Hydroxysteroid Dehydrogenases/genetics , Hyperglycemia/metabolism , Hyperinsulinism/metabolism , Insulin Resistance , Leptin/metabolism , Lipid Metabolism , Lipids/blood , Lipoprotein Lipase/genetics , Lipoprotein Lipase/metabolism , Male , Mice , Mice, Transgenic , Receptors, Glucocorticoid/genetics , Receptors, Glucocorticoid/metabolism , Viscera , Weight Gain
10.
J Biol Chem ; 276(44): 41293-300, 2001 Nov 02.
Article in English | MEDLINE | ID: mdl-11546766

ABSTRACT

Excess tissue glucocorticoid action may underlie the dyslipidemia, insulin resistance, and impaired glucose tolerance of the metabolic syndrome. 11beta-Hydroxysteroid dehydrogenase type 1 (11beta-HSD-1) catalyzes conversion of circulating inert 11-dehydrocorticosterone into active corticosterone, thus amplifying local intracellular glucocorticoid action, particularly in liver. The importance of 11beta-HSD-1 in glucose homeostasis is suggested by the resistance of 11beta-HSD-1(-/-) mice to hyperglycemia upon stress or obesity, due to attenuated gluconeogenic responses. The present study further investigates the metabolic consequences of 11beta-HSD-1 deficiency, focusing on the lipid and lipoprotein profile. Ad lib fed 11beta-HSD-1(-/-) mice have markedly lower plasma triglyceride levels. This appears to be driven by increased hepatic expression of enzymes of fat catabolism (carnitine palmitoyltransferase-I, acyl-CoA oxidase, and uncoupling protein-2) and their coordinating transcription factor, peroxisome proliferator-activated receptor-alpha (PPARalpha). 11beta-HSD-1(-/-) mice also have increased HDL cholesterol, with elevated liver mRNA and serum levels of apolipoprotein AI. Conversely, liver Aalpha-fibrinogen mRNA levels are decreased. Upon fasting, the normal elevation of peroxisome proliferator-activated receptor-alpha mRNA is lost in 11beta-HSD-1(-/-) mice, consistent with attenuated glucocorticoid induction. Despite this, crucial oxidative responses to fasting are maintained; carnitine palmitoyltransferase-I induction and glucose levels are similar to wild type. Refeeding shows exaggerated induction of genes encoding lipogenic enzymes and a more marked suppression of genes for fat catabolism in 11beta-HSD-1(-/-) mice, implying increased liver insulin sensitivity. Concordant with this, 24-h refed 11beta-HSD-1(-/-) mice have higher triglyceride but lower glucose levels. Further, 11beta-HSD-1(-/-) mice have improved glucose tolerance. These data suggest that 11beta-HSD-1 deficiency produces an improved lipid profile, hepatic insulin sensitization, and a potentially atheroprotective phenotype.


Subject(s)
Glucose Tolerance Test , Hydroxysteroid Dehydrogenases/metabolism , Insulin Resistance , Lipids/blood , Lipoproteins/blood , Liver/metabolism , 11-beta-Hydroxysteroid Dehydrogenases , Animals , Base Sequence , DNA Primers , Fibrinogen/metabolism , Hydroxysteroid Dehydrogenases/genetics , Mice , Mice, Knockout , Transcription, Genetic
11.
J Mol Endocrinol ; 22(2): 173-84, 1999 Apr.
Article in English | MEDLINE | ID: mdl-10194520

ABSTRACT

Leptin is a cytokine secreted from adipose tissue at a rate commensurate with the size of the body's fat stores. In addition to its anorectic and thermogenic central actions, leptin is known to act on peripheral tissues, including the pancreatic beta-cell where it inhibits insulin secretion and reduces insulin transcript levels. However, the role of leptin signalling through its full-length receptor, OB-Rb, in the beta-cell remains unclear. In the present study, we show that leptin activates a signal transducer and activator of transcription (STAT)3 signalling mechanism in pancreatic islets and in a rat model of the pancreatic beta-cell, RINm5F. Leptin induced DNA binding to a STAT consensus oligonucleotide and resulted in transcriptional activation from STAT reporter constructs in a manner consistent with STAT3 activation. Western blot analysis confirmed activation of STAT3 in RINm5F and isolated rat islets. Conditions that mimic increased metabolic activity resulted in attenuation of leptin-mediated STAT DNA binding but had no significant effect on STAT3 tyrosine phosphorylation in RINm5F cells. In addition, leptin activated the mitogen activated protein (MAP) kinase pathway in RINm5F cells. The present study provides a framework for OB-Rb signalling mechanisms in the programming of the beta-cell by leptin and suggests that increased metabolic activity may modulate this function.


Subject(s)
Insulin/metabolism , Islets of Langerhans/metabolism , Proteins/metabolism , Animals , Base Sequence , Binding Sites/genetics , Calcium/metabolism , Clone Cells , Cyclic AMP/metabolism , DNA/genetics , DNA/metabolism , DNA-Binding Proteins/genetics , DNA-Binding Proteins/metabolism , Diabetes Mellitus, Type 2/etiology , Diabetes Mellitus, Type 2/physiopathology , Humans , In Vitro Techniques , Insulin Secretion , Islets of Langerhans/cytology , Leptin , Male , Obesity/complications , Obesity/physiopathology , Rats , Rats, Wistar , STAT3 Transcription Factor , Signal Transduction , Trans-Activators/genetics , Trans-Activators/metabolism , Transcriptional Activation
12.
FEBS Lett ; 442(1): 57-60, 1999 Jan 08.
Article in English | MEDLINE | ID: mdl-9923604

ABSTRACT

Interleukin-1beta (IL-1beta) is a potent inflammatory cytokine involved in type 1 diabetes and acts through defined IL-1beta signaling pathways. In the present work we describe induction of DNA binding activity to signal transducer and activator of transcription (STAT) in response to IL-1beta in clonal insulin-secreting cells. Moreover, IL-1beta activates a short isoform of STAT-3 that potently stimulates transcription. Immunoprecipitation studies reveal an interaction between the activated STAT-3 and the IL-1 receptor accessory protein indicating an association between the two signaling pathways. This may be a novel point of transduction cross talk and an additional mechanism utilised by IL-1beta in the pancreatic beta-cell during the process of type 1 diabetes.


Subject(s)
DNA-Binding Proteins/metabolism , Insulin/metabolism , Interleukin-1/pharmacology , Trans-Activators/metabolism , Base Sequence , Clone Cells , DNA/genetics , DNA/metabolism , Humans , Insulin Secretion , Interleukin-1/metabolism , Interleukin-1 Receptor Accessory Protein , Islets of Langerhans/cytology , Islets of Langerhans/drug effects , Islets of Langerhans/physiology , Protein Binding , Proteins/metabolism , Receptors, Interleukin-1/metabolism , STAT3 Transcription Factor , Signal Transduction , Transfection
14.
J Biol Chem ; 273(40): 26194-201, 1998 Oct 02.
Article in English | MEDLINE | ID: mdl-9748302

ABSTRACT

The adipocyte hormone leptin activates signal transducer and activator of transcription 3 (STAT3) in the hypothalamus, mediating increased satiety and increased energy expenditure. To date, leptin-mediated activation of the STAT pathway in vivo has not been established in tissues other than hypothalamus. We now describe leptin receptor expression and in vivo signaling in discrete regions of the mouse gastrointestinal tract. Expression of the functional isoform leptin receptor (OB-Rb) is restricted to the jejunum and is readily detected by RT-PCR in isolated enterocytes from this site. Intravenous injection of leptin rapidly induced nuclear STAT5 DNA binding activity in jejunum of +/+ and obese (ob/ob) mice but had no effect in the diabetic (db/db) mouse that lacks the OB-Rb isoform. In addition, an induction of the immediate-early gene c-fos is observed in jejunum in vivo. Leptin-mediated induction of a number of immediate-early genes and activation of STAT3 and STAT5 in a human model of small intestine epithelium, CACO-2 cells, corroborate this effect. Furthermore, intravenous leptin administration caused a significant 2-fold reduction in the apolipoprotein AIV transcript levels in jejunum 90 min after a fat load. Our results suggest that the epithelium of jejunum is a direct target of leptin action, and this activity is dependent on the presence of OB-Rb. Lack of leptin or resistance to leptin action in this site may contribute to obesity and its related syndromes by directly affecting lipid handling.


Subject(s)
Jejunum/drug effects , Milk Proteins , Proteins/pharmacology , Receptors, Cell Surface , Animals , Apolipoproteins A/metabolism , Caco-2 Cells , Carrier Proteins/metabolism , DNA-Binding Proteins/analysis , DNA-Binding Proteins/metabolism , Dietary Fats , Female , Gene Expression Regulation/drug effects , Genes, fos/genetics , Humans , Injections, Intravenous , Leptin , Lipid Metabolism , Mice , Mice, Inbred Strains , Mice, Obese , Obesity/etiology , RNA, Messenger/drug effects , Receptors, Leptin , STAT3 Transcription Factor , STAT5 Transcription Factor , Trans-Activators/metabolism , Transcription, Genetic/drug effects
15.
Biochem Biophys Res Commun ; 238(3): 851-5, 1997 Sep 29.
Article in English | MEDLINE | ID: mdl-9325180

ABSTRACT

In addition to its interaction at hypothalamic sites to affect feeding and energy expenditure, leptin has been shown to exhibit a proliferative response in erythropoietic cells. The functional leptin receptor is also present in pancreatic islets and we now demonstrate that a commonly used clonal insulin secreting beta-cell line, RINm5F, expresses high levels of the Ob-Rb mRNA. Leptin causes an increase in tyrosine phosphorylation of a number of intracellular proteins and a dose related (10 nM-200 nM) increase in expression of the immediate-early gene, c-fos. This precedes a leptin induced proliferative response in serum-deprived RINm5F cells, which suggests that leptin might be involved in the complex regulation of proliferation of the pancreatic beta-cell.


Subject(s)
Carrier Proteins/biosynthesis , Islets of Langerhans/metabolism , Receptors, Cell Surface , Receptors, Cytokine/biosynthesis , Animals , Carrier Proteins/physiology , Cell Division/drug effects , DNA Replication/drug effects , Insulinoma , Obesity/metabolism , Phosphorylation , Phosphotyrosine/metabolism , Proto-Oncogene Proteins c-fos/genetics , Rats , Receptors, Cytokine/physiology , Receptors, Leptin , Tumor Cells, Cultured
16.
Biochem Biophys Res Commun ; 238(1): 267-70, 1997 Sep 08.
Article in English | MEDLINE | ID: mdl-9299491

ABSTRACT

The ob gene product leptin over the concentration range 0.1-100 nM demonstrated a U-shaped dose-response inhibition of glucose-stimulated insulin secretion by rat pancreatic islets. Thus, leptin (1 and 10 nM) produced a significant inhibition whereas 100 nM was ineffective. The inhibitory effect of leptin was glucose dependent, had a rapid onset and was readily reversed upon removal of leptin. Sub-chronic exposure of islets to leptin (10 nM) reduced both insulin secretion and the level of insulin transcript. These findings support the hypothesis that excessive production of leptin by adipose tissue could play a role in the development of non-insulin dependent diabetes in obese subjects.


Subject(s)
Insulin/genetics , Insulin/metabolism , Islets of Langerhans/metabolism , Proteins/pharmacology , RNA, Messenger/drug effects , Receptors, Cell Surface , Animals , Carrier Proteins/biosynthesis , Cell Separation , Dose-Response Relationship, Drug , Insulin Secretion , Islets of Langerhans/drug effects , Isomerism , Leptin , Male , Mice , Molecular Sequence Data , Obesity/metabolism , Proteins/metabolism , RNA, Messenger/biosynthesis , Rats , Rats, Wistar , Receptors, Leptin
17.
Diabetes ; 46(2): 313-6, 1997 Feb.
Article in English | MEDLINE | ID: mdl-9000710

ABSTRACT

Leptin, encoded for by the mouse ob gene, regulates feeding behavior and energy metabolism. Its receptor (Ob-R) is encoded by the mouse diabetic (db) gene and is mutated in the db/db mouse so that it lacks the cytoplasmic domain. We show that the full-length leptin receptor (Ob-Rb), which is believed to transmit the leptin signal, is expressed in pancreatic islets of ob/ob and wild-type mice, as well as in hypothalamus, liver, kidney, spleen, and heart. Recombinant leptin inhibited basal insulin release in the perfused pancreas preparation from ob/ob mice but not in that from Zucker fa/fa rats. Leptin (1-100 nmol/l) also produced a dose-dependent inhibition of glucose-stimulated insulin secretion by isolated islets from ob/ob mice. In contrast, leptin at maximum effective concentration (100 nmol/l) did not inhibit glucose-stimulated insulin secretion by islets from db/db mice. These results provide evidence that a functional leptin receptor is present in pancreatic islets and suggest that leptin overproduction, particularly from abdominal adipose tissue, may modify directly both basal and glucose-stimulated insulin secretion.


Subject(s)
Carrier Proteins/genetics , Insulin/metabolism , Islets of Langerhans/metabolism , Receptors, Cell Surface , Animals , Culture Techniques , Gene Expression , Insulin Secretion , Leptin , Mice , Mice, Obese , Proteins/pharmacology , Rats , Rats, Mutant Strains , Receptors, Leptin , Recombinant Proteins/pharmacology , Secretory Rate/drug effects , Tissue Distribution
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