ABSTRACT
BACKGROUND: A reliable early marker is required for diagnosis of cobalamin deficiency. We calculated an appropriate holotranscobalamin (HoloTC) cut-off point for identifying cobalamin deficiency using an immunoenzymatic assay. METHODS: Determination of the cut-off threshold and correlation between HoloTC and the other diagnostic parameters routinely used for vitamin B(12) deficiency [total vitamin B(12) (tB(12)), folate, homocysteine] were measured in 250 routine blood specimens from 107 men (mean age 59.0+/-18.8 years) and 143 women (mean age 54.2+/-23.1 years). The inclusion criterion was serum tB(12) concentration Subject(s)
Blood Chemical Analysis/instrumentation
, Transcobalamins/metabolism
, Vitamin B 12 Deficiency/blood
, Vitamin B 12/blood
, Aged
, Biomarkers/blood
, Biomarkers/metabolism
, Female
, Folic Acid/blood
, Folic Acid/metabolism
, Homocysteine/blood
, Homocysteine/metabolism
, Humans
, Immunoenzyme Techniques
, Male
, Middle Aged
, Sensitivity and Specificity
, Vitamin B 12/metabolism
, Vitamin B 12 Deficiency/diagnosis
, Vitamin B 12 Deficiency/metabolism
, Vitamin B Complex/blood
, Vitamin B Complex/metabolism
