ABSTRACT
Antiviral factor (AF) of protein nature has been isolated from chick embryo fibroblasts infected with Venezuelan equine encephalitis virus. The suppression of virus reproduction has been observed both in homologous and heterologous cell cultures when the preparation was introduced immediately after the adsorption of the virus after pretreatment of the cell monolayer. The study has demonstrated that the antiviral effect of AF is not linked with its IFN-alpha and TNF-alpha activity. Analysis of the results obtained in this study and earlier data contained in literature suggests that infected chick embryo fibroblasts release original cytokine of non-interferon nature with antiviral activity.
Subject(s)
Cytokines/pharmacology , Animals , Cell Line , Chick Embryo , Cytokines/isolation & purification , Encephalitis Virus, Venezuelan Equine/drug effects , Encephalitis Virus, Venezuelan Equine/physiology , Fibroblasts/immunology , Fibroblasts/microbiology , Humans , Interferon Inducers/pharmacology , Interferon-alpha/analysis , L Cells/immunology , L Cells/microbiology , Mice , Molecular Weight , Time Factors , Tumor Necrosis Factor-alpha/analysis , Vesicular stomatitis Indiana virus/drug effects , Vesicular stomatitis Indiana virus/physiology , Virus Cultivation , Virus Replication/drug effectsABSTRACT
An antiviral factor of protein nature inhibiting reproduction of VEE, herpes, vaccinia VSV, fowl plague viruses was isolated from infected cell cultures. The factor has no virucidal or prophylactic effect, stable to low pH values and heating at 100 degrees C for 30 min.
Subject(s)
Antiviral Agents/isolation & purification , Animals , Antiviral Agents/analysis , Antiviral Agents/pharmacology , Cells, Cultured , Chemical Phenomena , Chemistry, Physical , DNA Viruses/drug effects , Electrophoresis, Polyacrylamide Gel , Encephalomyelitis, Venezuelan Equine/microbiology , RNA Viruses/drug effects , Virus Cultivation , Virus Replication/drug effectsSubject(s)
Influenza, Human/prevention & control , Interferon Inducers/therapeutic use , Interferons/therapeutic use , Animals , Drug Evaluation , Drug Evaluation, Preclinical , Humans , Influenza A virus/drug effects , Influenza A virus/pathogenicity , Influenza, Human/microbiology , Interferon Inducers/pharmacology , Interferons/blood , Interferons/pharmacology , Mice , Microbial Sensitivity Tests , Polyribonucleotides/pharmacology , Polyribonucleotides/therapeutic use , Virulence/drug effectsABSTRACT
Certain high and low molecular interferon inducers poly(I), poly(C), polyguacyl, RFf2, dsRNA, tiloron) possess a marked antiinfluenza effect. The level of protection of the infected animals reached 70-80% under optimal condition of the use of the inducers. There was a clear-cut correlation between titres of serum interferon and the animal survival rate.
Subject(s)
Interferon Inducers/therapeutic use , Orthomyxoviridae Infections/drug therapy , Animals , Drug Evaluation, Preclinical , Interferons/blood , Mice , Orthomyxoviridae Infections/bloodABSTRACT
The therapeutic effectiveness of levamisole, megasine, and combination of these drugs was studied in 74 patients with genital herpes. Interferon production was first established in the patients treated with levamisole-megasine combination. A direct correlation was established between the therapeutic effectiveness of the drugs and interferon production. Most patients showed varying degrees of the positive effect in treatment with these drugs without any marked side effects. Recurrent genital herpes must be treated with levamisole and megasine for long periods of time.
Subject(s)
Gossypol/analogs & derivatives , Herpes Genitalis/drug therapy , Levamisole/administration & dosage , Adult , Drug Therapy, Combination , Female , Gossypol/administration & dosage , Humans , Male , Middle AgedABSTRACT
Interferon-inducing activity of polyanions (dextran Sulfate, native DNA, polyphosphate, polypentose, polyanetole sulfate, heparin) was studied. Dextran sulfate applied parenterally and enterally was found to be capable of inducing endogenous interferon production in white mice. The maximal titers of interferon (160 units/ml) were seen in the blood serum of mice upon administering the preparation in a dose of 20 mg/kg body mass. No substantial differences were discovered in the time course of interferon production whatever the route of administration.
Subject(s)
Dextrans/immunology , Interferon Inducers/immunology , Animals , Cells/drug effects , Dextran Sulfate , Dose-Response Relationship, Immunologic , Encephalitis Virus, Venezuelan Equine/immunology , Interferons/blood , Mice , Virus CultivationABSTRACT
The results of study on 2 Soviet interferon inductors, i.e. the synthetic polyguacyl polynucleotide and natural double-strand phage RNA or dsRNA were studied. It was shown that the time course of accumulation and period of circulation of interferon depended on the route of the inductor administration. The antiviral activity of polyguacyl and dsRNA in experimental influenza and tick-borne encephalitis is described. The maximum protective effect with respect to experimental influenza was observed with intranasal administration of the drugs 4 hours before inoculation. A pronounced protective effect with respect to tick-borne encephalitis was observed with intraperitoneal administration of the inductors or their use in the form of aerosols. Direct correlation between interferon production and the final protective effect was found.
