ABSTRACT
Colorectal tumors were studied 5 months after carcinogen injection to outbred albino rats with secondary immunodeficiency by common histological and immunobiochemical methods with the use of monoclonal and polyclonal antibodies to Ki-67, Bcl-2, p53, and VEGF. Injection of carcinogen to intact animals led to the formation of adenocarcinomas with high and moderate cell differentiation in the colon, while injection of carcinogen to animals with immunodeficiency was associated with a 1.5-fold higher incidence of GRADE 2 and GRADE 3 adenocarcinomas with more aggressive morphological phenotype and appearance of distant metastases.
Subject(s)
Antibodies/therapeutic use , Colorectal Neoplasms/drug therapy , Colorectal Neoplasms/immunology , 1,2-Dimethylhydrazine/toxicity , Adenocarcinoma/drug therapy , Adenocarcinoma/immunology , Adenocarcinoma/metabolism , Animals , Biomarkers, Tumor/metabolism , Cell Differentiation/drug effects , Colorectal Neoplasms/metabolism , Intestine, Large/drug effects , Intestine, Large/metabolism , Ki-67 Antigen/metabolism , Proto-Oncogene Proteins c-bcl-2/antagonists & inhibitors , Proto-Oncogene Proteins c-bcl-2/metabolism , Rats , Tumor Suppressor Protein p53/antagonists & inhibitors , Tumor Suppressor Protein p53/metabolism , Vascular Endothelial Growth Factor A/antagonists & inhibitors , Vascular Endothelial Growth Factor A/metabolismABSTRACT
The thymus of outbred male rats 5 months after splenectomy (experimental secondary immunodeficiency) was studied by common histological and immunohistochemical methods using monoclonal and polyclonal antibodies to CD3, CD30, CD68, synaptophysin, to S100, p53, bcl-2, and Ki-67 proteins. Removal of the spleen led to acute involution of the thymic parenchyma, which was replaced by the adipose tissue and was associated with restructuring of the thymopoietic and nonthymopoietic components of the gland, changes in cellular composition and antigenic phenotype of the lobular cortical and medullary matter, and by reduction of cell proliferation.
Subject(s)
Immunologic Deficiency Syndromes/metabolism , Immunologic Deficiency Syndromes/pathology , Thymus Gland/metabolism , Thymus Gland/pathology , Animals , Animals, Outbred Strains , Cell Count , Cell Proliferation , Immunohistochemistry , Immunologic Deficiency Syndromes/etiology , Macrophages/pathology , Male , Phenotype , Rats , Splenectomy , Thymocytes/pathology , Thymocytes/physiologyABSTRACT
This investigation was aimed at the study of splenic autotransplant cytoarchitectonics and cellular structures functional activity 3-60 days after the operation. The studies were carried out in 70 albino male rats including intact animals (n-10) and rats subjected to splenectomy and subsequent spleen heterotopic autotransplantation (n-60). Cryostat sections of transplants were processed for Falc and Cross luminescent-histochemical methods and were stained with hematoxylin and eosin. Biogenic amines content was measured using LUMAM-4 microscope with FMEL-1A spectrofluorimetric device. The number of luminescent cells in periarterial T-dependent zone was found to increase significantly after 7 days, while the unchanged lymphoid nodules were found in the zone of unaltered tissues. At day 15 "intrafollicular" luminescent granulocytes appeared in the newly formed lymphoid nodules. At days 15 to 45 the increased content of biogenic amines was detected in the "intrafollicular" cells, while the significant rise in the amount of amines was seen in the lymphocytes of nodule T-independent zone. It is suggested that the presence of intact nodules in transplant peripheral zone together with an increase in cell numbers in T-dependent zone, are the key events in the development of immune-correction effect of the transplanted spleen during the first two weeks after the operation.
