Extreme eutrophication and salinisation in the Coorong estuarine-lagoon ecosystem of Australia's largest river basin (Murray-Darling).

Estuaries in rainfall poor regions are highly susceptible to climatic and hydrological changes. The Coorong, a Ramsar-listed estuarine-coastal lagoon at the end of the Murray-Darling Basin (Australia), has experienced declining ecological health over recent decades. Twenty years of environmental data were analysed to assess patterns and drivers of water quality changes. Large areas of the Coorong are now persistently hyper-saline (salinity >80 psu) and hypereutrophic (total nitrogen, TN > 4 mg L-1, total phosphorus, TP > 0.2 mg L-1, chlorophyll a > 50 µg L-1) which coincided with reduced flushing due to diminished freshwater inflows and increasing evapo-concentration. Sediment quality also was related to flushing, with higher concentrations of organic carbon, TN, TP and sulfides as salinity increased. While total nutrient levels are very high, dissolved inorganic nutrients are generally low. Increased lagoonal flushing would be beneficial to reduce the hypersalinisation and hypereutrophication and improve ecosystem health.

The terminal lakes of the Murray River, Australia, were predominantly fresh before large-scale upstream water abstraction: Evidence from sedimentary diatoms and hydrodynamical modelling.

The Murray River is Australia's longest river, draining the continent's largest exoreic catchment. The river is Australia's most economically valuable, but is highly degraded by water extraction. The Murray River's terminal lakes, Lakes Alexandrina and Albert, formed following the mid-Holocene marine transgression. These lakes are part of one of the most ecologically important wetland ecosystems on the Australian continent and are recognised as internationally significant by the Ramsar Convention. As a result of upstream water extraction, the Lower Lakes are threatened by rising salinity. To combat this threat, water is allocated to maintain the Lower Lakes as freshwater ecosystems. This practice is part of the Murray-Darling Basin Plan, one of the largest environmental water allocation plans in the world. The water allocations and the natural history of the Lower Lakes are the subject of academic and public debate, since the water would otherwise be used for consumptive purposes, particularly irrigated agriculture, upstream. Recent modelling postulated that the lakes were saline for much of the period between 8500 and 5000 years ago. However, using new sedimentary diatom and hydrodynamic modelling evidence, we demonstrate that the Lower Lakes were fresh for most of this time, particularly after 7200 years ago. Elevated Murray River discharge between 7200 and 6600 years ago prevented sea water ingress, despite sea levels +1 m higher than present. After 6600 years ago, the lakes remained predominately fresh. Current management is, therefore, consistent with the lakes' history before European colonisation.

Global mapping of freshwater nutrient enrichment and periphyton growth potential.

Periphyton (viz. algal) growth in many freshwater systems is associated with severe eutrophication that can impair productive and recreational use of water by billions of people. However, there has been limited analysis of periphyton growth at a global level. To predict where nutrient over-enrichment and undesirable periphyton growth occurs, we combined several databases to model and map global dissolved and total nitrogen (N) and phosphorus (P) concentrations, climatic and catchment characteristics for up to 1406 larger rivers that were analysed between 1990 and 2016. We predict that 31% of the global landmass contained catchments may exhibit undesirable levels of periphyton growth. Almost three-quarters (76%) of undesirable periphyton growth was caused by P-enrichment and mapped to catchments dominated by agricultural land in North and South America and Europe containing 1.7B people. In contrast, undesirable periphyton growth due to N-enrichment was mapped to parts of North Africa and parts of the Middle East and India affecting 280 M people. The findings of this global modelling approach can be used by landowners and policy makers to better target investment and actions at finer spatial scales to remediate poor water quality owing to periphyton growth.

Comparative contributions of solution geochemistry, microbial metabolism and aquatic photosynthesis to the development of high pH in ephemeral wetlands in South East Australia.

The development of alkaline conditions in lakes and wetlands is common but the process of alkalinisation is not well elaborated. In this study we investigated causes of the seasonal alkalinisation of ephemeral wetlands in the South East of South Australia where pH values above 10 are frequently observed. This research combined field observations, geochemical analysis of wetland sediment and surface water, with mesocosm studies under controlled conditions. The results revealed a complex interplay between a number of different processes. A primary cause was attributed to sequestration of CO2 from the water column by plant photosynthesis, coupled with slow diffusion of CO2 from the air which led to its depletion in the water. Abundant plant growth also modified the water chemistry via uptake of nutrient elements, in particular calcium and magnesium and increased carbonate alkalinity in the water. Assessment of field results and geochemical modeling showed that low Ca/(HCO3(-) and CO3(-2)) ratios in the water, coupled with carbonate mineral (calcite, Mg substituted calcite, dolomite) precipitation and evapoconcentration, create a high alkalinity and pH (>9) baseline in many wetlands. The high baseline pH is then further increased by CO2 depletion due to photosynthesis. We could find no evidence that reduction of sulfate to sulfides by sulfur-reducing bacteria significantly contributed to the very high pH conditions.

MUC1 induces drug resistance in pancreatic cancer cells via upregulation of multidrug resistance genes.

MUC1 (CD227), a membrane tethered mucin glycoprotein, is overexpressed in >60% of human pancreatic cancers (PCs), and is associated with poor prognosis, enhanced metastasis and chemoresistance. The objective of this study was to delineate the mechanism by which MUC1 induces drug resistance in human (BxPC3 and Capan-1) and mouse (KCKO, KCM) PC cells. We report that PC cells that express high levels of MUC1 exhibit increased resistance to chemotherapeutic drugs (gemcitabine and etoposide) in comparison with cells that express low levels of MUC1. This chemo resistance was attributed to the enhanced expression of multidrug resistance (MDR) genes including ABCC1, ABCC3, ABCC5 and ABCB1. In particular, levels of MRP1 protein encoded by the ABCC1 gene were significantly higher in the MUC1-high PC cells. In BxPC3 and Capan-1 cells MUC1 upregulates MRP1 via an Akt-dependent pathway, whereas in KCM cells MUC1-mediated MRP1 upregulation is via an Akt-independent mechanism. In KCM, BxPC3 and Capan-1 cells, the cytoplasmic tail motif of MUC1 associates directly with the promoter region of the Abcc1/ABCC1 gene, indicating a possible role of MUC1 acting as a transcriptional regulator of this gene. This is the first report to show that MUC1 can directly regulate the expression of MDR genes in PC cells, and thus confer drug resistance.

Interventional therapy for acute myocardial infarction.

Percutaneous transluminal coronary angioplasty has proven to be more effective and safer than thrombolytic therapy for the treatment of acute ST elevation myocardial infarction. Coronary intervention decreases early mortality and the incidence of intracerebral hemorrhage when performed by an experienced interventional team in a timely fashion. After failed fibrinolytic therapy for myocardial infarction, percutaneous transluminal coronary angioplasty is indicated for signs of ischemia and is very effective in restoring vessel patency and reducing mortality when used as a rescue procedure. The glycoprotein IIb-IIIa inhibitors improve outcomes in percutaneous transluminal coronary angioplasty, particularly in patients undergoing stent placement. Percutaneous interventional therapy in acute myocardial infarction is particularly beneficial in patients with cardiogenic shock and effective for saphenous vein graft occlusions, patients with diabetes mellitus, and in the elderly. New devices and drugs are currently being tested for acute myocardial infarction and provide hope for even better interventional therapies in the near future.

Heart failure in women.

Heart failure is a major and growing public health problem in the United States. Hospitalization and death for heart failure have risen dramatically and the proportion of women with heart failure, especially older women, is projected to be the predominant group in the next 3 decades. There is growing evidence that the etiology, pathophysiology, prognosis, and response to treatment may all be different in women. In addition, there is evidence that hypertension plays a more prominent role and that preserved left ventricular systolic function is more likely to be found in women. There are legitimate questions about the degree of benefit that women receive from standard medications. A major obstacle in our understanding of heart failure in women has been their underrepresentation in major clinical trials. Current emphasis on ensuring adequate representation in clinical trials should help to further our understanding of heart failure in women and its management.