Subject(s)
Blood Glucose/physiology , Circadian Rhythm/physiology , Diabetes Mellitus/physiopathology , Hyperglycemia/physiopathology , Hypoglycemia/physiopathology , Diabetes Mellitus/blood , Diabetes Mellitus/drug therapy , Humans , Hyperglycemia/blood , Hyperglycemia/chemically induced , Hypoglycemia/blood , Hypoglycemia/chemically induced , Insulin/blood , Insulin/therapeutic useSubject(s)
Diabetes Mellitus, Type 1/diagnosis , Diabetes Mellitus, Type 2/diagnosis , Kidney Failure, Chronic/diagnosis , Diabetes Mellitus, Type 1/complications , Diabetes Mellitus, Type 2/complications , Diabetic Nephropathies/complications , Diabetic Nephropathies/diagnosis , Diabetic Retinopathy/complications , Diabetic Retinopathy/diagnosis , Female , Humans , Hyperlipoproteinemias/diagnosis , Hyperlipoproteinemias/etiology , Hypertension/diagnosis , Hypertension/etiology , Kidney Failure, Chronic/etiology , MaleSubject(s)
Cerebrovascular Disorders/etiology , Diabetes Complications , Adult , Age Factors , Aged , Aged, 80 and over , Cerebrovascular Disorders/epidemiology , Cerebrovascular Disorders/mortality , Diabetes Mellitus/epidemiology , Diabetes Mellitus/mortality , Female , Humans , Male , Middle Aged , Risk Factors , RomaniaABSTRACT
ATP-ase activity in mitochondria isolated from liver needle-biopsy samples was measured in 5 diabetics, aged 30-63 years and in 4 control subjects of similar age. The mitochondrial fraction was isolated by differential centrifugation of the homogenate and the ATP-ase activity was determined in the optimal conditions previously described for human liver mitochondria. The basal and Mg-stimulated ATP-ase activities were higher, while the DNP-stimulated ATP-ase activity was lower in diabetics compared to controls. The ratio of DNP-ATP-ase/Mg2+-ATP-ase was between 1-2 in diabetics and above 5 in controls. This pattern of ATP-ase activity in diabetics is indicative of mitochondrial damage. No quantitative changes in the amount of mitochondria isolated from liver (expressed in micrograms mitochondrial protein/mg wet tissue) could be noticed in diabetics compared to controls. Consequently, the alterations of ATP-ase activity is probably reflective of impairments of functional integrity of liver mitochondria in diabetics.