Zinc levels of patients with a moderate to severe COVID-19 infection at hospital admission and after 4th days of ward hospitalization and their clinical outcome.

BACKGROUND: Previous studies associate the disturbance of the Zinc (Zn) status with the severity of the disease and the inflammatory process in the critically ill patient. This decrease in Zn concentrations is an indicator of poor prognosis. Our aim was to evaluate Zn levels at admission and after four days, and to study if lower Zn levels at those days were related to a worse clinical outcome. MATERIAL AND METHODS: Observational cohort study at a tertiary Hospital. Recruitment period: 09/04/2020-04/24/2021. Clinical information on hypertension, diabetes mellitus (DM), chronic obstructive pulmonary disease (COPD), or bronchial asthma was collected. Obesity was defined as BMI ≥ 30 Kg/m2. Blood extraction was performed at admission and after 4 days. Zn was measured by atomic absorption using a flame method. Worse clinical outcome was defined as death during admission, intensive critical care unit admission or receiving supplemental oxygen through noninvasive or invasive ventilator care. RESULTS: 129 subjects were invited to participate but only 100 subjects completed the survey. According to ROC curve [AUC= 0.63 (95% CI 0.60-0.66)], Zn < 79 µg/dL showed the best performance to detect a worse outcome (Sn=0.85; Sp=0.36). Patients with Zn < 79 µg/dL were older (70 vs 61 y; p = 0.002) with no differences by sex. Most patients presented with fever, dysthermic symptoms and cough, without differences between groups. Pre-existing comorbid conditions did not differ significantly between groups. Less obese subjects were found in the Zn < 79 µg/dL group (21.4 vs 43.3%, p = 0.025). In the univariate analysis, Zn < 79 µg/dL at hospital admission was related to a worse outcome (p = 0.044), but after adjusting for age, C-reactive protein, and obesity there was no difference, but a tendency towards a worse prognosis [OR 2.20 (0.63-7.70), p = 0.215]. Zn levels increased in both groups after 4 days (66.6 vs 73.1 µg/dL at admission, and 72.2 vs 80.5 µg/dL at 4th day), with ns. difference (p = 0.214). CONCLUSION: Zn < 79 µg/dL at admission for a moderate to severe COVID-19 infection could be related to a worse outcome, although after adjustment for age, C-reactive protein levels and obesity, this Zn level threshold did not show statistically significant difference in the composite end point, but a tendency towards a worse prognosis. In addition, patients with the best clinical evolution showed higher serum Zn levels at 4th day after hospital admission than the patients with a worse prognosis.

The dawn of the Middle Paleolithic in Atapuerca: the lithic assemblage of TD10.1 from Gran Dolina.

The Atapuerca localities present evidence of a long series of hominin occupations from the Early Pleistocene onward and are a key site for understanding the continuity and discontinuity of Western European technological and settlement dynamics. The TD10 unit from Gran Dolina is located in the upper part of the sequence and divided into four lithostratigraphic subunits (TD10.4 to TD10.1, from bottom to top) dated between ca. 450 ka and ca. 250 ka (Marine Isotope Stage 11 to Marine Isotope Stage 8). The technological analysis of the lithic assemblages belonging to the TD10.1 sequence aims to determine the trends among its archeological levels and check its relation to late Middle Pleistocene technological evolution and site functionality. Archeostratigraphic studies have identified several occupation events within its approximately 1.5 m of thickness, whose artifact densities and occupational models differ. However, no remarkable technical differences have been observed among them. Lithic assemblages from those events show more evolved features than other Atapuerca Mode 2 assemblages. These changes are reflected in the selective raw material management strategies; more hierarchized and predetermined reduction methods; and the progressive decrease of large cutting tools in the lithic assemblages with respect to flake tools, the latter defined by a greater typological diversification. These technological changes did not lead to a clear break with respect to previous technological models and were accompanied by other sporadic but significant changes in subsistence and behavioral strategies (bone tools and retouchers; lithic recycling, and so on), which were consolidated during the Middle Paleolithic. Hence, the archeological record from the TD10.1 subunit of Gran Dolina reflects a local stratigraphic transition from Mode 2 to Mode 3 technocomplexes, paralleling that observed in other sites in southwestern Europe.

Shedding light on the Early Pleistocene of TD6 (Gran Dolina, Atapuerca, Spain): The technological sequence and occupational inferences.

This paper aims to update the information available on the lithic assemblage from the entire sequence of TD6 now that the most recent excavations have been completed, and to explore possible changes in both occupational patterns and technological strategies evidenced in the unit. This is the first study to analyse the entire TD6 sequence, including subunits TD6.3 and TD6.1, which have never been studied, along with the better-known TD6.2 Homo antecessor-bearing subunit. We also present an analysis of several lithic refits found in TD6, as well as certain technical features that may help characterise the hominin occupations. The archaeo-palaeontological record from TD6 consists of 9,452 faunal remains, 443 coprolites, 1,046 lithic pieces, 170 hominin remains and 91 Celtis seeds. The characteristics of this record seem to indicate two main stages of occupation. In the oldest subunit, TD6.3, the lithic assemblage points to the light and limited hominin occupation of the cave, which does, however, grow over the course of the level. In contrast, the lithic assemblages from TD6.2 and TD6.1 are rich and varied, which may reflect Gran Dolina cave's establishment as a landmark in the region. Despite the occupational differences between the lowermost subunit and the rest of the deposit, technologically the TD6 lithic assemblage is extremely homogeneous throughout. In addition, the composition and spatial distribution of the 12 groups of lithic refits found in unit TD6, as well as the in situ nature of the assemblage demonstrate the high degree of preservation at the site. This may help clarify the nature of the Early Pleistocene hominin occupations of TD6, and raise reasonable doubt about the latest interpretations that support the ex situ character of the assemblage as a whole.

In pursuit of our ancestors' hand laterality.

The aim of this paper is to apply a previously published method (Bargalló and Mosquera, 2014) to the archaeological record, allowing us to identify the hand laterality of our ancestors and determine when and how this feature, which is exhibited most strongly in humans, appeared in our evolutionary history. The method focuses on identifying handedness by looking at the technical features of the flakes produced by a single knapper, and discovering how many flakes are required to ascertain their hand preference. This method can potentially be applied to the majority of archaeological sites, since flakes are the most abundant stone tools, and stone tools are the most widespread and widely-preserved remains from prehistory. For our study, we selected two Spanish sites: Gran Dolina-TD10.1 (Atapuerca) and Abric Romaní (Barcelona), which were occupied by pre-Neanderthal and Neanderthal populations, respectively. Our analyses indicate that a minimum number of eight flakes produced by the same knapper is required to ascertain their hand preference. Even though this figure is relatively low, it is quite difficult to obtain from many archaeological sites. In addition, there is no single technical feature that provides information about handedness, instead there is a combination of eight technical features, localised on the striking platforms and ventral surfaces. The raw material is not relevant where good quality rocks are used, in this case quartzite and flint, since most of them retain the technical features required for the analysis. Expertise is not an issue either, since the technical features analysed here only correlate with handedness (Bargalló and Mosquera, 2014). Our results allow us to tentatively identify one right-handed knapper among the pre-Neanderthals of level TD10.1 at Gran Dolina (Atapuerca), while four of the five Neanderthals analysed from Abric Romaní were right-handed. The hand preference of the fifth knapper from that location (AR5) remains unclear.

The lithic industry of Sima del Elefante (Atapuerca, Burgos, Spain) in the context of Early and Middle Pleistocene technology in Europe.

This paper presents the lithic assemblages documented at Sima del Elefante (TE) and their importance in the context of the Early and Middle Pleistocene human occupation of Europe. We also study changes in human behaviour within the context of the palaeoenvironmental evolution of the Sierra de Atapuerca. This site has characteristics that are of great value for the study of human evolution. The lower levels of TE (Units TE7-TE14) are an essential reference for understanding the early stages of the colonization of Europe. The TE9c level has provided stone tools (Mode 1), faunal remains, and human fossils dated to 1.22 Ma (millions of years ago). Moreover, this is one of the few European sites with a stratigraphic sequence that includes remains of human occupations predating the Jaramillo subchron (Early Pleistocene) and from the Late Middle Pleistocene (Units TE18-TE19). Despite this, the presence of archaeologically sterile units (TE15-17) prevents us from establishing a continuous relationship between the Early and Middle Pleistocene human settlements and, consequently, between their technological and behavioural differences. We can, however compare the technological and palaeoeconomic strategies adopted by different species of hominins during two key phases of the occupation of Europe.

Age and date for early arrival of the Acheulian in Europe (Barranc de la Boella, la Canonja, Spain).

The first arrivals of hominin populations into Eurasia during the Early Pleistocene are currently considered to have occurred as short and poorly dated biological dispersions. Questions as to the tempo and mode of these early prehistoric settlements have given rise to debates concerning the taxonomic significance of the lithic assemblages, as trace fossils, and the geographical distribution of the technological traditions found in the Lower Palaeolithic record. Here, we report on the Barranc de la Boella site which has yielded a lithic assemblage dating to ∼1 million years ago that includes large cutting tools (LCT). We argue that distinct technological traditions coexisted in the Iberian archaeological repertoires of the late Early Pleistocene age in a similar way to the earliest sub-Saharan African artefact assemblages. These differences between stone tool assemblages may be attributed to the different chronologies of hominin dispersal events. The archaeological record of Barranc de la Boella completes the geographical distribution of LCT assemblages across southern Eurasia during the EMPT (Early-Middle Pleistocene Transition, circa 942 to 641 kyr). Up to now, chronology of the earliest European LCT assemblages is based on the abundant Palaeolithic record found in terrace river sequences which have been dated to the end of the EMPT and later. However, the findings at Barranc de la Boella suggest that early LCT lithic assemblages appeared in the SW of Europe during earlier hominin dispersal episodes before the definitive colonization of temperate Eurasia took place.

Can hand laterality be identified through lithic technology?

In this paper we present a new method for inferring handedness from lithic evidence. The study was conducted by means of an experimental programme in stone-knapping, after which the resulting lithic products (tools) were analysed. These lithic tools were produced by 15 inexpert knappers (8 right-handed and 7 left-handed), because we were not able to find a statistically significant number of left-handed expert knappers. We considered inexpert knappers to include individuals who had never struck two pebbles together, as well as individuals who were quite familiar with prehistoric tools and had had some degree of practice. The Mann-Whitney U test proved that all of them produced flakes with the same technical features, so within this sample expertise was not a factor that affected the presence or absence of the technical features analysed to determine hand laterality. The results of the experiment indicate that no single variable can be used to determine the laterality of the knapper, but rather the evidence of handedness lies in the combination of several variables. Furthermore, not all the flakes display the entire set of significant features. Therefore this study concludes that it is not possible to determine the handedness of a knapper through a single variable present on his or her flakes, but it may be possible to determine laterality by examining a combination of technical variables on a number of his or her pieces. Archaeologically, only well-preserved knapping events with numerous refitting products can be assigned to a left- or a right-handed knapper.

Hunting Activity Among Naturalistically Housed Chimpanzees (Pan troglodytes) at the Fundació Mona (Girona, Spain). Predation, Occasional Consumption and Strategies in Rehabilitated Animals.

Predatory behavior in wild chimpanzees and other primates has been well documented over the last 30 years. However, as it is an opportunistic behavior, conditions which may promote such behavior are left up to chance. Until now, predatory behavior among captive chimpanzees has been poorly documented. In this paper, we present five instances providing evidence of predatory behavior: four performed by isolated individuals and one carried out in cooperation. The evidence of group predation involved the chimpanzees adopting different roles as pursuers and ambushers. Prey was partially eaten in some cases, but not in the social episode. This study confirms that naturalistic environments allow chimpanzees to enhance species-typical behavioral patterns.

Population-level right-handedness for a coordinated bimanual task in naturalistic housed chimpanzees: replication and extension in 114 animals from Zambia and Spain.

Recently, many studies have been conducted on manual laterality in chimpanzees. Nevertheless, whether nonhuman primates exhibit population-level handedness remains a topic of considerable debate. One of the behaviors studied has been bimanual coordinated actions. Although recent studies have highlighted that captive chimpanzees show handedness at population level for these tasks, some authors have questioned the validity and consistency of these results. The first reason has been the humanization of the samples. The second one has been that the results refer to animals in American biomedical centers and the studies were conducted by the same team [WD Hopkins et al.]. This article aims to assess the laterality in bimanual coordination (tube task) activities in animals housed in an intermediate environment (Chimfunshi, Zambia). This has been conducted by replicating previous studies on similar samples (Mona Foundation, Spain), and then by extending the results to chimpanzees housed in intermediate settings. Individuals were evaluated through four experimental sessions (tests). Results indicated that 86% of the Chimfunshi sample was lateralized (48% RH, 38% LH). Furthermore, the sample showed population-level right-handedness in the mean handedness index, in Test 1, Test 2, and the first half of the study (Test 112). Rearing experience did not have an influence on handpreference. Taken together, the two sample (intermediate settings: Chimfunshi and Mona) results indicate a clear right-handedness. In conclusion, this replication and extension shows that (1) the Mona and Chimfunshi chimpanzees are right-handed in certain conditions, (2) the results are consistent with those obtained by Hopkins in captive settings, (3) the humanization of the samples does not affect manual laterality, (4) females are right-handed at population-level, but not males, and (5) these results reinforce the fact that the complexity of the task plays a dominant role in the expression of hand laterality among chimpanzees.

The first hominin of Europe.

The earliest hominin occupation of Europe is one of the most debated topics in palaeoanthropology. However, the purportedly oldest of the Early Pleistocene sites in Eurasia lack precise age control and contain stone tools rather than human fossil remains. Here we report the discovery of a human mandible associated with an assemblage of Mode 1 lithic tools and faunal remains bearing traces of hominin processing, in stratigraphic level TE9 at the site of the Sima del Elefante, Atapuerca, Spain. Level TE9 has been dated to the Early Pleistocene (approximately 1.2-1.1 Myr), based on a combination of palaeomagnetism, cosmogenic nuclides and biostratigraphy. The Sima del Elefante site thus emerges as the oldest, most accurately dated record of human occupation in Europe, to our knowledge. The study of the human mandible suggests that the first settlement of Western Europe could be related to an early demographic expansion out of Africa. The new evidence, with previous findings in other Atapuerca sites (level TD6 from Gran Dolina), also suggests that a speciation event occurred in this extreme area of the Eurasian continent during the Early Pleistocene, initiating the hominin lineage represented by the TE9 and TD6 hominins.

Ethological study of manual laterality in naturalistic housed chimpanzees (Pan troglodytes) from the Mona Foundation Sanctuary (Girona, Spain).

During recent years, handedness of nonhuman primates has been the subject of several studies, especially focused on our closest relatives: the chimpanzees. These studies have dealt with both wild and captive chimpanzees, and they seem to point to divergent conclusions, which have been interpreted as a by-product of the human influence in the captive samples. Here we present the results of a study of 10 chimpanzees (Pan troglodytes). In the past, they were trained in circus and marketing tasks (humanised behaviours), until they were confiscated and accepted into the Mona Foundation (in northeast Spain) in 2000, where they live in a semi-naturalistic environment. This study has been performed through observational bouts without systematic human influence, recording the actions carried out by chimpanzees when performing spontaneous activities. Our results indicate that chimpanzees that were under strong human influence in the past show the same trend in handedness as those living in freedom: few significant lateralities were observed among either individuals or tasks. So, laterality may not be influenced by humanisation. However, this conclusion must be taken as preliminary because very few individuals were studied.

Creatinuria: un indicador nutricional / Creatininuria: a nutritional index

Se estudiaron los índices de creatinina-peso y de creatinina-talla en 125 adolescentes de uno y otro sexos comprendidos entre las edades de 13 y 17 años. La determinación de la creatinina en la orina se llevó a efecto por el método del picrato. Se plantea que los valores medios de excreción en la orina son significativamente mayores en el sexo masculino. Se discute su utilidad como medio de apoyo en la evaluación del estado nutricional del individuo y se sugiere su ampliación a otros grupos etarios y actividades diferentes

Creatinuria. un indicador nutricional / Creatininuria: a nutritional index

Se estudiaron los índices de creatinina-peso y de creatinina-talla en 125 adolescentes de uno y otro sexos comprendidos entre las edades de 13 y 17 años. La determinación de la creatinina en la orina se llevó a efecto por el método del picrato. Se plantea que los valores medios de excreción en la orina son significativamente mayores en el sexo masculino. Se discute su utilidad como medio de apoyo en la evaluación del estado nutricional del individuo y se sugiere su ampliación a otros grupos etarios y actividades diferentes

Niveles de vitamina A en suero y en leche de un grupo de madres upoestamente sanas / Serum and milk vitamin A levels in a group of considered healthy mothers

Se presentan datos acerca de las concentraciones de vitamina A en suero sanguíneo y leche de un grupo de 107 madres supuestamente sanas, residentes en los municipios Centro Habana y Habana Vieja, que se siguieron desde el preparto hasta seis semanas despues del parto. La proporción de mujeres con concentraciones séricas de retinol consideradas como de "alto riesgo" al cabo de las seis semanas fue insignificante, por lo que no parece haber una deficiencia de vitamina A como problema de salud pública en el grupo estudiado. La concentración media de vitamina A en la leche materna es similar a los valores medios que suelen hallarse en grupos de madres con condiciones favorables de vida

Niveles de vitamina A en suero y en leche de un grupo de madres upoestamente sanas / Seru and milk vitamin A levels in a group of considered healthy mothers

Se presentan datos acerca de las concentraciones de vitamina A en suero sanguíneo y leche de un grupo de 107 madres supuestamente sanas, residentes en los municipios Centro Habana y Habana Vieja, que se siguieron desde el preparto hasta seis semanas despues del parto. La proporción de mujeres con concentraciones séricas de retinol consideradas como de "alto riesgo" al cabo de las seis semanas fue insignificante, por lo que no parece haber una deficiencia de vitamina A como problema de salud pública en el grupo estudiado. La concentración media de vitamina A en la leche materna es similar a los valores medios que suelen hallarse en grupos de madres con condiciones favorables de vida (AU)

Niveles séricos de vitamina A y ácido ascórbico en grupo de mujeres durante el embarazo / Serum vitamin A and ascorbic acid livels in a group of women during pregnancy

Se presentan los valores sérico de vitamina A y ácido ascórbico de un grupo de embarazadas supuestamente sanas en un área de salud de Centro Habana, que fueron estudiados longitudinalmente durante la gestación. La concentración de vitamina A en suero disminuyó significativamente (p<0,01) del segundo trimestre al tercero, pero la interpretación de este descenso es difícil, por no existir una definición acerca de los valores normales de esta variable durante el embarazo. La concentración media de ácido ascórbico en suero, por el contrario, se incrementó significativamente (p<0,01) ya en el segundo, trimestre, y se redujo la proporción de gestantes con valores considerados como de "alto" y "moderado riesgo", lo que demuestra una respuesta favorable a la suplementación que regularmente se indica en los servicios de atención primaria prenatal (AU)

Cambios del colesterol sérico en un grupo de obreros cortadores de caña de azúcar (macheteros) durante la zafra

Se estudió los cambios de los niveles de colesterol sérico en un grupo de obreros cortadores de caña de azúcar.Se investigó la influencia de una modificación brusca del régimen de vida, incluyendo la nutrición, sobre dichos niveles. Entre el comienzo y el final del período de investigaciones de halló un descenso estadísticamente significativo del colesterol sérico y de la tensión arterial diastólica. Se determinó una significativa correlación entre los cambios ocurridos en los niveles individuales de colesterol sérico y los valores inciales respectivos(AU)