ABSTRACT
Introduction into clinical practice of combined positron emission technology and computer tomography (PET/CT) allows in one study to identify structural and functional abnormalities. The study involves 32 patients who underwent PET/CT with "C-choline, including 5 patients with prostate cancer (PC), 3--with chronic prostatitis and 24--with biochemical PC recurrence. PET/CT with 11C-choline has a high diagnostic efficacy in detection of local recurrence and PC metastases in patients with biochemical PC recurrence. The results of visual analysis do not permit to distinguish PC from benign prostate diseases.
Subject(s)
Carbon Radioisotopes , Choline , Positron-Emission Tomography/methods , Prostatic Neoplasms/diagnostic imaging , Radiopharmaceuticals , Aged , Diagnosis, Differential , Humans , Male , Middle Aged , Prostatic Diseases/diagnostic imaging , Russia , Tomography, X-Ray ComputedABSTRACT
For the first time, the biodistribution of recombinant heat shock protein in rhHsp70 rats with grafted intracranial C6 glioma was evaluated. It was assessed using the fluorescent antibody accumulation chaperone rhHsp70 conjugated with fluorochrome Alexa Fluor 555 in tumor cells by intratumoral or intravenous administration. Assessment of the distribution and accumulation of labeled protein was carried out on the model of subcutaneous B16/F10 melanoma in C57BL/6 mice with the use of single-photon emission computer tomography. After 60 minutes after intravenous administration rhHsp70-I123 (20 MBq, 5 mg chaperone) accumulation of the drug mainly in the liver and tumor tissue was showed. The coefficient of the differential accumulation of the labeled protein KDN(tumor/background) was 3.14. It was turned out that comparing the level of fixation of rhHsp70-I123 in the liver and the tumor KDN(tumor/ liver) = 0.76. After 24 hours from the time of injection of rhHsp70-I123 it was observed increase the level of fixation of the labeled protein in the liver and melanoma: KDN(tumor/background) = 3.43; KDN(tumor/liver = 0.78.
Subject(s)
Brain Neoplasms/metabolism , Glioma/metabolism , HSP70 Heat-Shock Proteins/metabolism , Liver/metabolism , Melanoma, Experimental/metabolism , Skin Neoplasms/metabolism , Animals , Fluorescent Dyes , HSP70 Heat-Shock Proteins/administration & dosage , HSP70 Heat-Shock Proteins/pharmacokinetics , Injections, Intralesional , Injections, Intravenous , Iodine Radioisotopes , Male , Mice , Mice, Inbred C57BL , Neoplasms, Experimental/metabolism , Rats , Rats, Wistar , Time Factors , Tissue Distribution , Tomography, Emission-Computed, Single-PhotonABSTRACT
The paper describes 12 years' experience in using positron emission tomography (PET) at the Russian Research Center of Radiology and Surgical Technologies to detect cancer, cardiac, and psychoneurological diseases, to make their differential diagnosis, and to evaluate the efficiency of their treatment. It shows the capacities of PET using various radiopharmaceutical agents in a broad spectrum of the above abnormalities and defines prospects for further development of the technique.