ABSTRACT
BACKGROUND: Different species of Croton are used in traditional Amazonian medicine. Among the popular uses are treatment of bacterial diseases, poorly healing wounds and fevers. OBJECTIVE: This study evaluated the antileishmanial, antiplasmodial and antimicrobial activities of the extracts and diterpenes of Croton palanostigma Klotzsch (Euphorbiaceae). MATERIALS AND METHODS: Leaves and bark were extracted with dichloromethane and methanol. The bark dichloromethane extract (BDE) was chromatographed on a column, obtaining cordatin and aparisthman. The extracts and diterpenes were assayed thought agar disk diffusion method and their bactericidal or fungicidal effects were evaluated by minimum bactericidal or fungicidal concentration. The antiplasmodial activity was evaluated after 24 and 72 h of exposition. The antileishmanial activity was performed on promastigotes forms of Leishmania amazonensis. RESULTS: The bark methanol extract (BME) and cordatin were not active against any microbial strains tested; BDE and leaves methanol extract (LME) were positive for Pseudomonas aeruginosa and aparisthman was positive for Candida albicans. In the determination of the minimum bactericidal concentration, neither of them were active in the highest concentration tested. The extracts and diterpenes were inactive in Plasmodium falciparum, except the LME in 72 h. Any extract was shown to be active in promastigote forms of L. amazonensis. CONCLUSION: These results indicate that the BDE and LME did not inhibit the bacterial growth, then they probably had bacteriostatic effect. LME presented activity in P. falciparum.
ABSTRACT
BACKGROUND: Different species of Croton are used in traditional Amazonian medicine. Among the popular uses are treatment of bacterial diseases, poorly healing wounds and fevers. OBJECTIVE: This study evaluated the antileishmanial, antiplasmodial and antimicrobial activities of the extracts and diterpenes of Croton palanostigma Klotzsch (Euphorbiaceae). MATERIALS AND METHODS: Leaves and bark were extracted with dichloromethane and methanol. The bark dichloromethane extract (BDE) was chromatographed on a column, obtaining cordatin and aparisthman. The extracts and diterpenes were assayed thought agar disk diffusion method and their bactericidal or fungicidal effects were evaluated by minimum bactericidal or fungicidal concentration. The antiplasmodial activity was evaluated after 24 and 72 h of exposition. The antileishmanial activity was performed on promastigotes forms of Leishmania amazonensis. RESULTS: The bark methanol extract (BME) and cordatin were not active against any microbial strains tested; BDE and leaves methanol extract (LME) were positive for Pseudomonas aeruginosa and aparisthman was positive for Candida albicans. In the determination of the minimum bactericidal concentration, neither of them were active in the highest concentration tested. The extracts and diterpenes were inactive in Plasmodium falciparum, except the LME in 72 h. Any extract was shown to be active in promastigote forms of L. amazonensis. CONCLUSION: These results indicate that the BDE and LME did not inhibit the bacterial growth, then they probably had bacteriostatic effect. LME presented activity in P. falciparum.
ABSTRACT
The occurrence of human pulmonary dirofilariasis maintains a relation with the prevalence of Dirofilaria immitis infection in the canine population. Several mosquito species are vectors of this nematode. Canine blood samples collected in Pingo d'Agua and União villages, Salvaterra municipality (Marajó Island, Pará), in June, 2004 (n = 34) and April, 2005 (N = 90) were analyzed. Parasitological and immunological (ELISA--kit SNAP(R) 3DX Biobrasil) diagnoses were compared following the examination of 34 samples. The prevalence in the population (N = 90) was evaluated by means of ELISA. ELISA revealed more positive samples (25/34; 73.5%) than thick smears (23/34, 67.6%) or Knott (21/34, 61.8%), but the differences were not significant (p > 0.05). The frequency of D. immitis infection was 58% in dogs ranging from 0-2 years old, whereas in older dogs it was 100%. The prevalence of canine dirofilariasis was high in Pingo d'Agua and Vila União (53.5%), indicating the risk of parasite transmission to the people in this area.
Subject(s)
Antigens, Helminth , Dirofilaria immitis/isolation & purification , Dirofilariasis/epidemiology , Dog Diseases/epidemiology , Animals , Brazil/epidemiology , Dirofilaria immitis/immunology , Dirofilariasis/diagnosis , Dog Diseases/diagnosis , Dog Diseases/parasitology , Dogs , Enzyme-Linked Immunosorbent Assay , Feces/parasitology , Humans , Lung Diseases, Parasitic/transmission , Predictive Value of Tests , Prevalence , Risk Factors , Sensitivity and SpecificityABSTRACT
A ocorrência de dirofilariose pulmonar humana relaciona-se com a prevalência de infecção por Dirofilaria immitis na população canina. Várias espécies de mosquitos são vetores desse nematóide. Analisaram-se amostras de sangue canino coletados nas vilas Pingo d'Agua e União, município de Salvaterra (Ilha do Marajó, PA), em junho, 2004 (n=34) e abril, 2005 (N=90). Os diagnósticos parasitológico e imunológico (ELISA - kit SNAP® 3DX, Biobrasil) foram comparados (chi2, alfa=0,05) no exame de 34 amostras. A prevalência na população (N=90) foi avaliada pelo ELISA. O ELISA revelou mais positivos (25/34; 73,5 por cento) que a gota espessa (23/34, 67,6 por cento) e o Knott (21/34, 61,8 por cento), mas a diferença não foi significativa (p>0,05). A freqüência de infecção por D. immitis na faixa de 0 a 2 anos foi 58 por cento, enquanto em cães mais velhos foi 100 por cento. A prevalência da dirofilariose canina em Pingo d'Agua e Vila União foi alta (53,5 por cento), indicando risco de transmissão do parasito às pessoas nessa área.
The occurrence of human pulmonary dirofilariasis maintains a relation with the prevalence of Dirofilaria immitis infection in the canine population. Several mosquito species are vectors of this nematode. Canine blood samples collected in Pingo d'Agua and União villages, Salvaterra municipality (Marajó Island, Pará), in June, 2004 (n=34) and April, 2005 (N=90) were analyzed. Parasitological and immunological (ELISA - kit SNAP® 3DX, Biobrasil) diagnoses were compared following the examination of 34 samples. The prevalence in the population (N=90) was evaluated by means of ELISA. ELISA revealed more positive samples (25/34; 73.5 percent) than thick smears (23/34, 67.6 percent) or Knott (21/34, 61.8 percent), but the differences were not significant (p>0.05). The frequency of D. immitis infection was 58 percent in dogs ranging from 0-2 years old, whereas in older dogs it was 100 percent. The prevalence of canine dirofilariasis was high in Pingo d'Agua and Vila União (53.5 percent), indicating the risk of parasite transmission to the people in this area.
