ABSTRACT
Relationship of eremomycin biosynthesis to the quantity and quality of the inoculate (age, aeration rate, number of subcultures) was studied. The seed medium composition for cultivating the inoculate was defined. A procedure for cultivation of the seed material for biosynthesis of eremomycin providing an increase in the antibiotic yield by 24 per cent was developed.
Subject(s)
Actinomyces/metabolism , Anti-Bacterial Agents , Actinomyces/growth & development , Agar , Air , Carbon , Culture Media , Fermentation , Glycopeptides/biosynthesis , In Vitro Techniques , Nitrogen , Time Factors , USSRABSTRACT
The composition of the medium for biosynthesis of eremomycin was optimized with mathematical design of the experiment. Optimal concentrations of the medium components were determined with the method of orthogonal latin squares. The levels of eremomycin production on the medium were 60-89 per cent higher than those on the initial medium.
Subject(s)
Anti-Bacterial Agents/biosynthesis , Culture Media , Carbon/metabolism , Glycopeptides/biosynthesis , Nitrogen/metabolismABSTRACT
The stability of the tobramycin-producing organism was studied by the property of the antibiotic production. The organism was stored under conditions of different exposures to light and subculture on slants. The culture was also subjected to long-term storage with various methods. The organism was stable in preserving its antibiotic activity for 1.5 months when stored on the Gauze organic agar No. 2. Subcultures of the organism on this medium provided preservation of the antibiotic activity throughout 4 passages. The culture storage on millet provided preservation of the antibiotic activity for 6 months.
Subject(s)
Streptomyces/metabolism , Tobramycin/biosynthesis , Culture Media/metabolism , Fermentation , Preservation, Biological , Temperature , Time FactorsABSTRACT
The effect of temperature, aeration and active acidity of the medium on biosynthesis of tobramycin was studied. The optimal temperature conditions (37 degrees C) were developed. It was shown that biosynthesis of tobramycin depended on the aeration conditions, especially at the beginning of the fermentation process. The initial pH 4.9-6.35 and 6.35-7.1 were found to be optimal for the growth of the tobramycin-producing organism and biosynthesis of the antibiotic, respectively.
Subject(s)
Culture Media/pharmacology , Tobramycin/biosynthesis , Aerobiosis , Chemical Phenomena , Chemistry, Physical , Fermentation , Hydrogen-Ion Concentration , Streptomyces/metabolism , Temperature , Time FactorsABSTRACT
The optimal concentrations of mineral phosphorus for the growth of the tobramycin-producing organism and for the production of the antibiotic by it in the synthetic medium were determined. Introduction of an additional source of mineral phosphorus into the rich soybean medium resulted in decreased levels of antibiotic production. The stimulating effect of manganese sulfate on the biosynthesis of tobramycin in the rich medium was shown. The stimulating effect of soybean, linseed and palm oils on the production of tobramycin was evident when the fermentation period was longer.
Subject(s)
Tobramycin/biosynthesis , Culture Media , Fermentation , Linseed Oil , Manganese , Oils , Phosphorus , Glycine maxABSTRACT
The mathematical design of the experiment was used for optimization of the nutrient medium for biosynthesis of tobramycin by Streptomyces cremeus var. tobramycini. The use of the orthogonal Latin rectangles provided development of the fermentation medium composition and definition of the optimal concentration of its ingredients. The level of tobramycin production on this medium was 2 times higher than that on the initial one.
Subject(s)
Anti-Bacterial Agents/biosynthesis , Culture Media/pharmacology , Tobramycin/biosynthesis , Dose-Response Relationship, Drug , Fermentation , Mathematics , Research Design , Streptomyces/metabolismABSTRACT
The effect of the quality and quantity of the inoculum on the biosynthesis of tobramycin was studied. The medium with corn steep liquor and glycerol was found to be the optimal seed medium for the tobramycin-producing organism. It is required that the fermentation medium be supplemented with 2 per cent of the vegetative inoculum at the stationary growth phase or with an increased amount of the seed material at the exponential growth phase or at the early stage of the culture dying. Subculturing of the inoculum decreases the level of tobramycin production at the fermentation stage: when the culture of the 5th--6th generation is used, the activity of the fermentation broth decreases by 30--50 per cent.
Subject(s)
Anti-Bacterial Agents/biosynthesis , Culture Media/pharmacology , Tobramycin/biosynthesis , Fermentation , Hydrogen-Ion Concentration , Streptomyces/metabolism , Temperature , Time FactorsABSTRACT
The effect of the sources of carbon and inorganic nitrogen on the biosynthesis of tobrmycin complex was studied. Maltose and ammonium chloride were shown tobe optimal. A synthetic medium for Streptomyces cremeus var. nov. 2242, to tobramyc in producing organism, was elaborated and its optimization was performed using the experiment mathematical modelling, i. e. the method of latin squares. Tobramycin production on the new medium was 6 to 7 times higher than that on the initial medium.
