Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 20 de 74
Filter
Add more filters










Publication year range
1.
Biochemistry ; 40(43): 13114-23, 2001 Oct 30.
Article in English | MEDLINE | ID: mdl-11669650

ABSTRACT

We have investigated the role of the labile terminal domains of the core histones on the stability of the subunits of the protein core of the nucleosome by studying the thermodynamic behavior of the products of limited trypsin digestion of these subunits. The thermal stabilities of the truncated H2A-H2B dimer and the truncated (H3-H4)/(H3-H4)(2) system were studied by high-sensitivity differential scanning calorimetry and circular dichroism spectroscopy. The thermal denaturation of the truncated H2A-H2B dimer at pH 6.0 and low ionic strength is centered at 47.3 degrees C. The corresponding enthalpy change is 35 kcal/mol of 11.5 kDa monomer unit, and the heat capacity change upon unfolding is 1.2 kcal/(K mol of 11.5 kDa monomer unit). At pH 4.5 and low ionic strength, the truncated (H3-H4)/(H3-H4)(2) system, like its full-length counterpart, is quantitatively dissociated into two truncated H3-H4 dimers. The thermal denaturation of the truncated H3-H4 dimer is characterized by the presence of a single calorimetric peak centered at 60 degrees C. The enthalpy change is 25 kcal/mol of 10 kDa monomer unit, and the change in heat capacity upon unfolding is 0.5 kcal/(K mol of 10 kDa monomer unit). The thermal stabilities of both types of truncated dimers exhibit salt and pH dependencies similar to those of the full-length proteins. Finally, like their full-length counterparts, both truncated core histone dimers undergo thermal denaturation as highly cooperative units, without the involvement of any significant population of melting intermediates. Therefore, removal of the histone "tails" does not generally affect the thermodynamic behavior of the subunits of the core histone complex, indicating that the more centrally located regions of the histone fold and the extra-fold structured elements are primarily responsible for their stability and responses to parameters of their chemical microenvironment.


Subject(s)
Histones/chemistry , Animals , Calorimetry, Differential Scanning , Circular Dichroism , Dimerization , Dose-Response Relationship, Drug , Hydrogen-Ion Concentration , Kinetics , Protein Binding , Protein Structure, Tertiary , Salts/pharmacology , Sodium Chloride/pharmacology , Temperature , Thermodynamics , Trypsin/pharmacology , Ultraviolet Rays , Urea/pharmacology
2.
Arch Biochem Biophys ; 364(2): 161-6, 1999 Apr 15.
Article in English | MEDLINE | ID: mdl-10190970

ABSTRACT

The absorption spectra of Ni(II) bound to the core histone tetramer, (H3-H4)2, of chicken erythrocytes in 500 mM NaCl + 100 mM phosphate (pH 7.4) were recorded. A charge transfer band was seen at 317 nm, characteristic of a bond between Ni(II) and the sulfur atom of Cys-110 of histone H3. The conditional affinity constants for Ni(II) binding at pH 7.4 for low and high Ni(II) saturation (log Kc = 4.26 +/- 0.02 and 5.26 +/- 0.11 M-1, respectively) were calculated from spectrophotometric titrations with the use of this band. The binding of Ni(II) to (H3-H4)2 is proposed to involve the Cys-110 and His-113 of different H3 molecules within the tetramer. The competition between histones and low-molecular-weight chelators for Ni(II) in the cell nucleus, histidine and glutathione, is discussed on the basis of the above results, indicating that histone H3 is very likely to bind Ni(II) dissolved intracellularly from phagocytosed particulate nickel compounds.


Subject(s)
Histones/metabolism , Nickel/metabolism , Polymers/metabolism , Animals , Binding, Competitive , Chickens , Spectrophotometry, Ultraviolet
3.
EMBO J ; 16(9): 2493-506, 1997 May 01.
Article in English | MEDLINE | ID: mdl-9171362

ABSTRACT

Within the core histone octamer each histone H4 interacts with each H2A-H2B dimer subunit through two binding surfaces. Tyrosines play a central role in these interactions with H4 tyrosines 72 and 88 contacting one H2A-H2B dimer subunit, and tyrosine 98 contacting the other. To investigate the roles of these interactions in vivo, we made site-directed amino acid substitutions at each of these tyrosine residues. Elimination of either set of interactions is lethal, suggesting that binding of the tetramer to both dimers is essential. Temperature-sensitive mutants were obtained through single amino acid substitutions at each of the tyrosines. The mutants show both strong positive and negative effects on transcription. Positive effects include Spt- and Sin-phenotypes resulting from mutations at each of the three tyrosines. One allele has a strong negative effect on the expression of genes essential for the G1 cell cycle transition. At restrictive temperature, mutant cells fail to express the CLN1, CLN2, SWI4 and SWI6 genes, and have reduced levels of CLN3 mRNA. These results demonstrate the critical role of histone dimer-tetramer interactions in vivo, and define their essential role in the expression of genes regulating G1 cell cycle progression.


Subject(s)
Gene Expression Regulation , Genes, Fungal/genetics , Histones/genetics , Saccharomyces cerevisiae/genetics , Chromatin/chemistry , Crystallography, X-Ray , DNA Mutational Analysis , DNA, Fungal/chemistry , DNA, Fungal/genetics , Dimerization , Fungal Proteins/genetics , G1 Phase , Histones/chemistry , Models, Molecular , Mutagenesis, Site-Directed , Nucleosomes/metabolism , Phenotype , Protein Conformation , Transcription, Genetic , Transcriptional Activation , Tyrosine/genetics
4.
Chromosoma ; 106(6): 335-47, 1997 Nov.
Article in English | MEDLINE | ID: mdl-9362542

ABSTRACT

A rabbit antiserum, specific for the histone H3.3 replacement variant, was raised with the aid of a histone H3.3-specific peptide. Immuno blot experiments demonstrated the specificity of this polyclonal antiserum. In addition, we showed on immuno blots that two monoclonal antibodies isolated from mice with systemic lupus erythematosus (SLE) display strong reactivity with the H3.3 histone, but not with its replication-dependent counterparts. Our observations indicate that histone H3.3 might play a role as autoantigen in SLE. We used the histone H3.3-specific antiserum to characterize the germ line chromatin in cytological preparations of Drosophila testes, because our previous studies had shown that a histone H3.3-encoding gene is strongly expressed in the germ line of Drosophila males. The antiserum reacted with some of the lampbrush loops in spermatocytes and with chromatin of the postmeiotic germ cells of males. Our data indicate that histone H3.3 is not evenly distributed throughout the chromatin of germ cells, but is concentrated in distinct regions. Histone H3.3 disappears from the spermatid nuclei, along with the other core histones, during the late stages of spermatogenesis. In Drosophila polytene chromosomes, however, a rather uniform distribution of the histone H3.3 was observed. The possible role of histone H3.3 is discussed.


Subject(s)
Chromatin/chemistry , Drosophila/chemistry , Histones/analysis , Spermatocytes/chemistry , Amino Acid Sequence , Animals , Antibodies, Monoclonal , Antibody Specificity , Drosophila/physiology , Drosophila melanogaster/physiology , Histones/chemistry , Immune Sera , Lupus Erythematosus, Systemic/immunology , Male , Meiosis , Mice , Models, Molecular , Molecular Sequence Data , Rabbits , Spermatogenesis
5.
Science ; 274(5287): 614-7, 1996 Oct 25.
Article in English | MEDLINE | ID: mdl-8849453

ABSTRACT

Histone-DNA contacts within a nucleosome influence the function of trans-acting factors and the molecular machines required to activate the transcription process. The internal architecture of a positioned nucleosome has now been probed with the use of photoactivatable cross-linking reagents to determine the placement of histones along the DNA molecule. A model for the nucleosome is proposed in which the winged-helix domain of the linker histone is asymmetrically located inside the gyres of DNA that also wrap around the core histones. This domain extends the path of the protein superhelix to one side of the core particle.


Subject(s)
DNA/chemistry , Histones/chemistry , Nucleosomes/chemistry , Animals , Base Sequence , Binding Sites , Cross-Linking Reagents , DNA/metabolism , Histones/metabolism , Models, Molecular , Molecular Sequence Data , Nucleic Acid Conformation , Protein Conformation , Protein Structure, Secondary , RNA, Ribosomal/genetics , Recombinant Proteins/chemistry , Xenopus
6.
Biochemistry ; 35(6): 2037-46, 1996 Feb 13.
Article in English | MEDLINE | ID: mdl-8639689

ABSTRACT

The self-associative behavior and the thermal stability of the H3/H4 histone complex was studied in low-ionic strength conditions by several physicochemical techniques, including differential scanning calorimetry and circular dichroism spectroscopy. At neutrality, the major molecular species present in solution is the (H3-H4)2 tetramer. Its thermodynamic properties cannot be studied directly though, since its thermal denaturation is completely irreversible even at the lowest salt concentrations. However, a complete thermodynamic analysis can be performed at low ionic strength and pH 4.5, where the (H3-H4)2 tetramer is quantitatively dissociated into two H3-H4 dimers and where almost complete reversibility of the thermal transitions is attained. The unfolding transition temperature of the 26.5 kDa H3-H4 dimer increases as a function of both the ionic strength of the solvent and the total protein concentration. The thermal denaturation of the H3-H4 dimer is characterized by the presence of a single calorimetric peak, centered at 58 degrees C, with a corresponding enthalpy change of 25 kcal/mol of a 13 kDa monomer unit and a change in heat capacity upon unfolding of about 0.6 kcal/(K mol of 13 kDa monomer unit). The complex between histones H3 and H4 (tetramer or dimer) is stable between pH 9.5 and 3.0. At pH 1.5, the system is almost completely unfolded at all temperatures. At low ionic strengths and pH values between 5.0 and 2.5, the H3-H4 dimer behaves as a highly cooperative system, melting as a single unit; i.e. individual H3 and H4 folded monomers are not detectable during the treatment. The two-state mechanism accounting for the unfolding of the H3-H4 dimer at pH 4.5 is the same as that described for the H2A-H2B dimer at neutrality. Just like for the H2A and H2B histones, the H3 and H4 polypeptides are properly folded only when assembled as H3-H4 dimers or in higher-order histone assemblies. Therefore, coupling along the interfaces of the two chains within the heterodimer is the major factor contributing to the stabilization of the secondary and tertiary structures of the chains as well as of the histone dimers.


Subject(s)
Histones/chemistry , Animals , Calorimetry, Differential Scanning , Chemical Phenomena , Chemistry, Physical , Chickens , Circular Dichroism , Drug Stability , Hydrogen-Ion Concentration , In Vitro Techniques , Osmolar Concentration , Protein Conformation , Protein Denaturation , Thermodynamics
8.
Genes Dev ; 9(22): 2770-9, 1995 Nov 15.
Article in English | MEDLINE | ID: mdl-7590252

ABSTRACT

Transcription of many yeast genes requires the SWI/SNF regulatory complex. Prior studies show that reduced transcription of the HO gene in swi and snf mutants is partially relieved by mutations in the SIN1 and SIN2 genes. Here we show that SIN2 is identical to HHT1, one of the two genes coding for histone H3, and that mutations in either can result in a Sin- phenotype. These mutations are partially dominant to wild type and cause amino acid substitutions in three conserved positions in the structured domain of histone H3. We have also identified partially dominant sin mutations that affect two conserved positions in the histone-fold domain of histone H4. Three sin mutations affect surface residues proposed to interact with DNA and may reduce affinity of DNA for the histone octamer. Two sin mutations affect residues at or near interfaces between (H2A-H2B) dimer and (H3-H4)2 tetramer subunits of the histone octamer and may affect nucleosome stability or conformation. The ability of mutations affecting the structure of the histone octamer to relieve the need for SWI and SNF products supports the proposal that the SWI/SNF complex stimulates transcription by altering chromatin structure and can account for the apparent conservation of SWI and SNF proteins in eukaryotes other than yeast.


Subject(s)
Amino Acids/metabolism , Chromosomal Proteins, Non-Histone/genetics , DNA-Binding Proteins/genetics , Genes, Fungal , Histones/metabolism , Saccharomyces cerevisiae Proteins , Transcription, Genetic , Base Sequence , DNA Primers , Fungal Proteins/metabolism , Genetic Complementation Test , Histones/chemistry , Histones/genetics , Molecular Sequence Data , Mutation , Phenotype , Trans-Activators/metabolism , Transcription Factors/metabolism
9.
Proc Natl Acad Sci U S A ; 92(24): 11170-4, 1995 Nov 21.
Article in English | MEDLINE | ID: mdl-7479959

ABSTRACT

The histones of all eukaryotes show only a low degree of primary structure homology, but our earlier crystallographic results defined a three-dimensional structural motif, the histone fold, common to all core histones. We now examine the specific architectural patterns within the fold and analyze the nature of the amino acid residues within its functional segments. The histone fold emerges as a fundamental protein dimerization motif while the differentiations of the tips of the histone dimers appear to provide the rules of core octamer assembly and the basis for nucleosome regulation. We present evidence for the occurrence of the fold from archaebacteria to mammals and propose the use of this structural motif to define a distinct family of proteins, the histone fold superfamily. It appears that evolution has conserved the conformation of the fold even through variations in primary structure and among proteins with various functional roles.


Subject(s)
DNA-Binding Proteins/ultrastructure , Histones/ultrastructure , Nucleosomes/ultrastructure , Protein Structure, Secondary , Protein Structure, Tertiary , Amino Acid Sequence , Animals , Bacterial Proteins/chemistry , Biological Evolution , Models, Molecular , Molecular Sequence Data , Protein Binding , Sequence Alignment , Sequence Homology, Amino Acid
10.
Nucleic Acids Res ; 23(14): 2685-91, 1995 Jul 25.
Article in English | MEDLINE | ID: mdl-7651829

ABSTRACT

The histone fold motif has previously been identified as a structural feature common to all four core histones and is involved in both histone-histone and histone-DNA interactions. Through the use of a novel motif searching method, a group of proteins containing the histone fold motif has been established. The proteins in this group are involved in a wide variety of functions related mostly to DNA metabolism. Most of these proteins engage in protein-protein or protein-DNA interactions, as do their core histone counterparts. Among these, CCAAT-specific transcription factor CBF and its yeast homologue HAP are two examples of multimeric complexes with different component subunits that contain the histone fold motif. The histone fold proteins are distantly related, with a relatively small degree of absolute sequence similarity. It is proposed that these proteins may share a similar three-dimensional conformation despite the lack of significant sequence similarity.


Subject(s)
DNA-Binding Proteins/chemistry , Histones/chemistry , Amino Acid Sequence , Animals , DNA/metabolism , DNA-Binding Proteins/genetics , DNA-Binding Proteins/metabolism , Histones/genetics , Histones/metabolism , Humans , Molecular Sequence Data , Molecular Structure , Protein Binding , Protein Conformation , Protein Folding , Sequence Homology, Amino Acid
11.
Biochemistry ; 34(17): 5988-96, 1995 May 02.
Article in English | MEDLINE | ID: mdl-7727455

ABSTRACT

The thermal stability of the core histone dimer H2A-H2B has been studied by high-sensitivity differential scanning calorimetry and circular dichroism spectroscopy. The unfolding transition temperature of the 28 kDa H2A-H2B dimer increases as a function of both the ionic strength of the solvent and the total protein concentration. At neutral pH and physiological ionic strength, the thermal denaturation is centered at about 50 degrees C with a corresponding enthalpy change of about 40 kcal/mol of 14 kDa monomer unit and an excess heat capacity of about 1.4 kcal/(K.mol) of 14 kDa monomer unit. The H2A-H2B dimer is stable mainly between pH 5.5 and 10.5. Below pH 4.0, the system is unfolded at all temperatures. The thermodynamic analysis is performed at low ionic strength where almost complete reversibility is attained, since higher salt conditions seem to promote aggregation and irreversibility of the transitions. Analysis of the data shows that at low ionic strength and pH values between 6.5 and 8.5, the H2A-H2B dimer behaves as a highly cooperative system, melting as a single unit without any detectable intermediates of dissociated, yet folded, H2A and H2B monomers. This is consistent with the observed protein concentration dependence of the midpoint of the thermal denaturation. The two-state unfolding process can be described by the general scheme AB-->2U, indicating that the individual H2A and H2B polypeptides are folded, stable entities only when complexed as the H2A-H2B dimer and that the major contribution to the stabilization of the dimer derives from the coupling between the H2A and H2B interfaces.


Subject(s)
Histones/chemistry , Animals , Calorimetry, Differential Scanning , Chickens , Circular Dichroism , Hydrogen-Ion Concentration , Macromolecular Substances , Osmolar Concentration , Protein Folding , Thermodynamics
12.
J Mol Biol ; 236(1): 179-88, 1994 Feb 11.
Article in English | MEDLINE | ID: mdl-8107103

ABSTRACT

The crystal structure of the histone octamer has now been determined at 3.1 A resolution and refined to a crystallographic R value of 25.5%. The overall shape of the structure is significantly different from that originally reported by Burlingame et al. and its length is now in agreement with that observed by Klug et al. in their low-resolution studies. The experimental intensity data used in constructing the new electron density map were the same as those used by Burlingame et al. for the original electron density map. In addition, the methods used in producing the new density map were also the same as those for the original map. The only difference between the two calculations was the selection of the heavy-atom location. The large change seen in the structural image (110 A x 70 A x 70 A versus 55 A x 70 A x 70 A) was due to a relatively small change (2.27 A shift) of the heavy-atom site. The fact that the shape and size of the original structure were incorrect is surprising and unusual, since the electron density map that produced the original model was clear for most parts of the structure; one could easily see the well-formed right-handed helices of the H2A and H2B molecules, and the ordered parts of the H2A and H2B molecules could be easily traced from end to end. A comparison of the two maps shows that the original image was derived from two fused copies of the correct structure rotated by +/- 120 degrees from its true location along a rotation axis parallel to the z-axis and the image seen was a partial (about 19.5%) overlap of two molecules. An explanation is given as to how such a small shift of the heavy-atom position could create such a double image in the unit cell, and how the original electron density map could be converted to the new map by a phase modification in the Fourier synthesis. This study resolves the differences between the analyses of the shape and size of the histone octamer structure.


Subject(s)
Histones/chemistry , Nucleosomes/chemistry , Protein Conformation , Crystallography, X-Ray/methods , Fourier Analysis , Mathematics , Models, Theoretical
13.
Proc Natl Acad Sci U S A ; 90(22): 10489-93, 1993 Nov 15.
Article in English | MEDLINE | ID: mdl-8248135

ABSTRACT

The histone octamer core of the nucleosome is a protein superhelix of four spirally arrayed histone dimers. The cylindrical face of this superhelix is marked by intradimer and interdimer pseudodyad axes, which derive from the nature of the histone fold. The histone fold appears as the result of a tandem, parallel duplication of the "helix-strand-helix" motif. This motif, by its occurrence in the four dimers, gives rise to repetitive structural elements--i.e., the "parallel beta bridges" and the "paired ends of helix I" motifs. A preponderance of positive charges on the surface of the octamer appears as a left-handed spiral situated at the expected path of the DNA. We have matched a subset of DNA pseudodyads with the octamer pseudodyads and thus have built a model of the nucleosome. In it, the two DNA strands coincide with the path of the histone-positive charges, and the central 12 turns of the double helix contact the surface of the octamer at the repetitive structural motifs. The properties of these complementary contacts appear to explain the preference of histones for double-helical DNA and to suggest a possible basis for allosteric regulation of nucleosome function.


Subject(s)
Histones/ultrastructure , Nucleosomes/ultrastructure , Binding Sites , DNA/metabolism , DNA-Binding Proteins/chemistry , Helix-Loop-Helix Motifs , In Vitro Techniques , Macromolecular Substances , Models, Molecular , Protein Binding , Protein Conformation
15.
Biochemistry ; 31(44): 10844-51, 1992 Nov 10.
Article in English | MEDLINE | ID: mdl-1420197

ABSTRACT

Gel filtration and sedimentation studies have previously established that the vertebrate animal core histone octamer is in equilibrium with an (H3-H4)2 tetramer and an H2A-H2B dimer [Eickbush, T. H., & Moudrianakis, E. N. (1978) Biochemistry 17, 4955-4964; Godfrey, J. E., Eickbush, T. H., & Moudrianakis, E. N. (1980) Biochemistry 19, 1339-1346]. We have investigated the core histone octamer of wheat (Triticum aestivum L.) and have found it to be much more stable than its vertebrate animal counterpart. When vertebrate animal histone octamers are subjected to gel filtration in 2 M NaCl, a trailing peak of H2A-H2B dimer can be clearly resolved from the main octamer peak. When the plant octamer is subjected to the identical procedure, there is no trailing peak of H2A-H2B dimer, but rather a single peak containing the octamer. A sampling across the octamer peak from leading to trailing edge shows no change in the ratio of H2A-H2B to (H3-H4)2. Surprisingly, the plant octamer shows the same stability at 0.6 M NaCl, a salt concentration in which the vertebrate animal octamer dissociates into dimers and tetramers. Equilibrium sedimentation data indicate that the assembly potential of the wheat histones in 2 M NaCl is very high at all protein concentrations above 0.1 mg mL-1. In order to disrupt the forces stabilizing the plant histone octamer at high histone concentrations, the concentration of NaCl must be lowered to approximately 0.3 M.(ABSTRACT TRUNCATED AT 250 WORDS)


Subject(s)
Histones/chemistry , Nucleosomes/chemistry , Plants/ultrastructure , Animals , Chickens/blood , Chromatography, Gel , Drug Stability , Macromolecular Substances , Molecular Weight , Sodium Chloride/pharmacology , Triticum
16.
Proc Natl Acad Sci U S A ; 88(22): 10148-52, 1991 Nov 15.
Article in English | MEDLINE | ID: mdl-1946434

ABSTRACT

The structure of the octameric histone core of the nucleosome has been determined by x-ray crystallography to a resolution of 3.1 A. The histone octamer is a tripartite assembly in which a centrally located (H3-H4)2 tetramer is flanked by two H2A-H2B dimers. It has a complex outer surface; depending on the perspective, the structure appears as a wedge or as a flat disk. The disk represents the planar projection of a left-handed proteinaceous superhelix with approximately 28 A pitch. The diameter of the particle is 65 A and the length is 60 A at its maximum and approximately 10 A at its minimum extension; these dimensions are in agreement with those reported earlier by Klug et al. [Klug, A., Rhodes, D., Smith, J., Finch, J. T. & Thomas, J. O. (1980) Nature (London) 287, 509-516]. The folded histone chains are elongated rather than globular and are assembled in a characteristic "handshake" motif. The individual polypeptides share a common central structural element of the helix-loop-helix type, which we name the histone fold.


Subject(s)
Histones/chemistry , Nucleosomes/ultrastructure , Animals , Chickens , Computer Graphics , Computer Simulation , Erythrocytes/ultrastructure , Macromolecular Substances , Models, Molecular , Photography , Protein Conformation , X-Ray Diffraction/methods
17.
Biochemistry ; 30(36): 8817-23, 1991 Sep 10.
Article in English | MEDLINE | ID: mdl-1888742

ABSTRACT

Mixtures of histones H3 and H4 were examined by analytical ultracentrifugation and circular dichroism to determine their association behavior and secondary structure content in high and low ionic strength solvents containing chloride, phosphate, or sulfate. H3 and H4 were also cross-linked by using DSP in order to directly trap any intermolecular interactions occurring in solution. While H3 and H4 can exist as an H3-H4 dimer under limited conditions, they behave as a stable (H3-H4)2 tetramer under most conditions, particularly those which are physiologically relevant. In chloride-containing solutions, the equilibrium between H3-H4 and (H3-H4)2 is responsive to changes in ionic strength and paralleled by large changes in alpha-helicity. In sulfate- and phosphate-containing solutions, the equilibrium is again governed by ionic strength, but there are no significant changes in secondary structure accompanying shifts in the equilibrium. Small oligomers can be formed in the presence of sulfate and phosphate and trapped by the cross-linking reagent; these oligomers are much smaller than those formed in chloride-containing solutions. However, addition of the H2A-H2B dimer into the system prevents aggregation of the (H3-H4)2 tetramer by acting as a "molecular cap" and thus regulating the assembly pathway toward the formation of tripartite octamers. The observed assembly of H3 and H4 into a stable, tetrameric complex supports the concept of the core histone octamer having a tripartite organization in solution rather than being organized as two heterotypic tetramers.


Subject(s)
Histones/chemistry , Protein Conformation , Animals , Chickens , Chlorides , Circular Dichroism , Cross-Linking Reagents , Erythrocytes/chemistry , Histones/genetics , Histones/metabolism , Hydrogen-Ion Concentration , Osmolar Concentration , Phosphates , Structure-Activity Relationship , Sulfates , Ultracentrifugation
18.
J Biol Chem ; 266(9): 5696-702, 1991 Mar 25.
Article in English | MEDLINE | ID: mdl-2005107

ABSTRACT

The structure of the nucleosome has been under intense investigation using neutron crystallography, x-ray crystallography, and neutron solution scattering. However the dimension of the histone octamer inside the nucleosome is still a subject of controversy. The radius of gyration (Rg) of the octamer obtained from solution neutron scattering of core particles at 63% 2H2O, 37% 1H2O is 33 A, and x-ray crystallography study of isolated histone octamer gives a Rg of 32.5 A, while the reported values using x-ray crystallography of core particles from two individual studies are 29.7 and 30.4 A, respectively. We report here studies of isolated histone octamer and trypsin-limited digested octamer using both neutron solution scattering and small angle x-ray scattering. The Rg of the octamer obtained is 33 A, whereas that of the trimmed octamer is 29.8 A, similar to the structure obtained from the crystals of the core particles. The N-terminal domains of the core histones in the octamer have been shown by high resolution nuclear magnetic resonance (Schroth, G.P., Yau, P., Imai, B.S., Gatewood, J.M., and Bradbury, E.M. (1990) FEBS Lett. 268, 117-120) to be mobile and flexible; it is likely that these regions are disordered and "not seen" by x-ray crystallography.


Subject(s)
Histones , Nucleosomes , Animals , Chickens , Crystallography , Electrophoresis, Polyacrylamide Gel , Erythrocytes/chemistry , X-Ray Diffraction
19.
Biochemistry ; 29(4): 973-6, 1990 Jan 30.
Article in English | MEDLINE | ID: mdl-2340288

ABSTRACT

The circular dichroism (CD) of freshly prepared chicken erythrocyte core histones has been reexamined in high concentrations of ammonium sulfate and sodium chloride, conditions which cause drastic changes in the solubility and aggregative properties of these proteins. After sample clarification by ultracentrifugation, no significant net changes are detected in the secondary structure of the core histones in the range of 2.0-2.5 M ammonium sulfate. There is also no significant difference between the CD spectra of histone solutions in 2 M sodium chloride and clarified solutions of histones in high concentrations of ammonium sulfate. It was observed that sample clarification by ultracentrifugation immediately prior to taking CD spectra was necessary for signal stabilization, especially under conditions which begin to favor crystallization of the histones.


Subject(s)
Histones , Ammonium Sulfate/pharmacology , Animals , Buffers , Chemical Phenomena , Chemistry, Physical , Chickens , Circular Dichroism , Cold Temperature , Molecular Structure , Osmolar Concentration , Sodium Chloride/pharmacology , Solutions
20.
Biochemistry ; 29(4): 965-72, 1990 Jan 30.
Article in English | MEDLINE | ID: mdl-2187535

ABSTRACT

The secondary structure of the calf thymus core histone octamer, (H2A-H2B-H3-H4)2, and its two physiological subunits, the H2A-H2B dimer and (H3-H4)2 tetramer, was analyzed by ORD spectropolarimetry as a function of temperature and solvent ionic strength within the ranges of these experimental parameters where assembly of the core histone octamer exhibits pronounced sensitivity. While the secondary structure of the dimer is relatively stable from 0.1 to 2.0 M NaCl, the secondary structure of the tetramer exhibits complex changes over this range of NaCl concentrations. Both complexes exhibit only modest responses to temperature changes. ORD spectra of very high and very low concentrations of stoichiometric mixtures of the core histones revealed no evidence of changes in the ordered structure of the histones as a result of the octamer assembly process at NaCl concentrations above 0.67 M, nor were time-dependent changes detected in the secondary structure of tetramer dissolved in low ionic strength solvent. The secondary structure of the chicken erythrocyte octamer dissolved in high concentrations of ammonium sulfate, including those of our crystallization conditions, was found to be essentially unchanged from that in 2 M NaCl when examined by both ORD and CD spectropolarimetry. The two well-defined cleaved products of the H2A-H2B dimer, cH2A-H2B and cH2A-cH2B, exhibited reduced amounts of ordered structure; in the case of the doubly cleaved moiety cH2A-cH2B, the reductions were so pronounced as to suggest marked structural rearrangements.


Subject(s)
Histones/metabolism , Ammonium Sulfate/pharmacology , Animals , Cattle , Chickens , Circular Dichroism , Endopeptidases/metabolism , Erythrocytes/analysis , Molecular Structure , Optical Rotatory Dispersion , Osmolar Concentration , Temperature
SELECTION OF CITATIONS
SEARCH DETAIL
...