ABSTRACT
We performed allogenic bone marrow transplantation (BMT) in two adult T-cell leukemia (ATL) patients with HLA-identical siblings as donors. One patient, with acute ATL, relapsed 3 months after BMT. The other, with chronic ATL, has become free of disease over 18 months after the BMT from his human T-cell lymphotropic virus type 1 (HTLV-1)-negative sibling, and we were unable to detect HTLV-1 in the patient's peripheral blood. Based on our results and those of others, although there have been limited numbers of patients, BMT may represent the only potentially curative treatment for ATL, and the presence of graft-vs.-host disease tends to be related to good results, which suggests the possibility that graft-vs.-leukemia effects may play an important role in allogenic BMT for ATL.
Subject(s)
Bone Marrow Transplantation , Leukemia, T-Cell/therapy , Adult , Female , Graft vs Tumor Effect , Human T-lymphotropic virus 1/isolation & purification , Humans , Leukemia, T-Cell/pathology , Leukemia, T-Cell/virology , Male , Recurrence , Transplantation, Homologous , Treatment OutcomeABSTRACT
To determine the optimal time of peripheral blood stem cell harvest (PBSCH), we evaluated the usefulness of the detection of immature hematopoietic cells (immature leukocytes and reticulocytes) using newly developed automatic cell analyzers, and compared the results with those of conventional CD34 measurement by flow cytometry (FCM). The presence of immature leukocytes and reticulocytes was calculated as immature leukocyte information (IMI)-positive rate and high fluorescence reticulocyte (HFR)-positive rate by multi-item autoanalyzers. Both the IMI-positive rate and HFR-positive rate positively correlated with the CD34-positive rate and was more highly correlated with the colony forming units-mixed (CFU-Mix) count in peripheral blood. In addition, they positively correlated not only with the CD34-positive rate, but also with the colony forming units-granulocyte/macrophage (CFU-GM) count and CFU-Mix count in harvested stem cell juice. These findings imply that the parameters of IMI and HFR could be used for determining the optimal time of PBSCH, and could also be useful for quantifying stem cells.
